Assessment of the microbiological activity in agricultural and urban soils

  • 1 Division of Agricultural, Food and Forestry Engineering, Faculty of Civil and Environmental Engineering, Białystok University of Technology, Wiejska 45A, 15-351 Białystok, Poland


The aim of this study was to evaluate the enzymatic soil and the number of selected microorganisms in urban soil, which are located in the lane of the reconstructed road and compare it with a soil cultivated for agricultural purposes. The conducted analysis showed significant differences between the results of the soil taken from the roadway and the soil cultivated from agricultural purposes. The C:N ratio in soils of the roadway (from 24 to 31) indicated that they were degraded and heavily degraded soils. Urban soils had a neutral pH. The activity of dehydrogenase (1.93–6.95 μg TPF g−1·h−1), acid phosphatase (2.42–4.92 mM pNP·g−1·h−1) and alkaline phosphatase (2.34–4.80 mM pNP·g−1·h−1) in urban soils were low. In agricultural soils the acid phosphatase enzyme levels ranged 6.32–8.04 mM pNP·g−1·h−1, and alkaline phosphatase were 7.26–9.16 mM pNP·g−1·h−1. In urban soil samples collected along the roadway, a significant correlation between potassium and dehydrogenase activity, and between the C:N ratio and the activity of acid phosphatase was found.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Baath E., 1989. Effects of heavy metals in soil on microbial process and populations (a review). Water Air Soil Pollution, 47: 335–379.

  • Barabasz W., Vořišek K., 2002. Biodiversity of microorganisms in soil environments. The activity of microorganisms in different environments. AR Krakow: 23–34.

  • Baran S., 2000. Assessment of degradation and soil remediation. Exercise Guide. Ed. AR, Lublin.

  • Bielińska E.J., Mocek A., 2003. Soil enzyme activity operated orchard as an indicator of the environment caused by the use of plastic mulches. Notebooks Problematic Progress of Agricultural Sciences, 492: 25–37.

  • Dick W.A., Tabatabai M.A., 1993. Significance and potential uses of soil enzymes. [W:] Soil microbial ecology: application in agricultural and environmental management. Ed. F.B. Metting. Dekker, New York: 95–125.

  • Gostkowska K., Furczak J., Domżał H., Bielińska E.J., 1998: Suitability of some biochemical and microbiological tests for the degradation degree of podzolic soil on the back-ground of it differentiated usage. Polish Journal of Soil Science, 30(2): 69–78.

  • Januszek K., 1999. The enzymatic activity of selected southern Polish forest soils under field and laboratory studies. Exercise Sciences. AR Krakow, 250.

  • Kieliszewska-Rokicka B., 2001. Soil Enzymes and their role in the study of biological activity of the soil. [In:] Micro-organisms of the soil environment. Collective work. Editor. H. Dahn, A. Pokojska-Burdziej. Toruń: Publisher. A. Marshal: 37–47.

  • Kobus J., 1995. Biological processes and the formation of soil fertility. Notebooks Problematic Progress of Agricultural Sciences, 421a: 209–219.

  • Koper J., Piotrowska A., 1996. Soil enzyme activity of deer, depending on the crops in crop rotation and monoculture. Soil Science Annual, 3: 89–100.

  • Kozanecka T., Rekosz-Burlaga H., Russel S., 1996. Microbiological activity in an apple orchard, depending on how its maintenance, fertilization and liming. Soil Science Annual, 47: 75–84.

  • Mocek A., Mocek-Płóciniak A., 2010. Xenobiotics in soil Polish. Science Nature Technologies, 4/6.

  • Mrozowska J., 1999. Laboratory of general and environmental microbiology. Ed. PSL, Gliwice.

  • Myśków W., Stachyra A., Ziąba S., Masiak D., 1996. The biological activity of the soil as an indicator of soil fertility and fertility. Soil Science Annual 47, 1/2: 89–99.

  • Nannipieri P., Grego S., Ceccanti B., 1990. Ecological significance of the biological activity in soil. [W:] Soil Biochemistry, 6. Ed. J.M. Bollag, G. Stotzky. Dekker, New York: 293–355.

  • PN-ISO 13878:2002. Soil quality – Determination of total nitrogen content after dry combustion („elemental analysis”).

  • PN-ISO 14235:2003P. Soil quality. Determination of the content of organic carbon by dichromate oxidation (VI) in a sulfuric acid (VI).

  • PN-ISO 10390:1997. Soil quality – Determination of pH.

  • PN-R-04022:1996/Az1:2002. Agricultural chemical analysis of the soil – Determination of available potassium in mineral soils.

  • PN-R-04023:1996. Agricultural chemical analysis of the soil – Determination of available phosphorus in mineral soils.

  • PN-R-04020:1994/Az1:2004. Agricultural chemical analysis of the soil – Determination of available magnesium.

  • Russel S., 2005. The importance of research of enzymes in soil. Acta Agrophysica, 3: 5–9.

  • Sándor G., Szabó G., 2014. Influence of human activities on the soils of Debrecen, Hungary. Soil Science Annual, 65(1): 2–9.

  • Szember A., 2001. Outline of agricultural microbiology. Publisher Agricultural University in Lublin, Lublin.

  • Siuta J., 1995. Soils, diagnose the condition and threats. Institute of Environmental Protection, Warsaw: 112.

  • Smolik B., Nowak J., 2003. An attempt to establish indicator of pollution of soil petroleum products through the study of some enzymes of soil. Notebooks Problematic Progress of Agricultural Sciences, 492: 311–319.

  • Tabatabai M.A., 1994. Enzymes. [W:] Methods of soil analysis. Part 2. Microbiological and biochemical properties. 5. Red. R.W. Weaver, S. Augle, P. J. Bottomly, D. Bezdicek, S. Smith, M.A. Tabatabai, A. Wollum. Soil Science Society of America, Madison: 755–833.

  • Tabatabai M.A., Bremner J.M., 1969. Use of p-nitrophenylphosphate for assay of soil phosphatase activity. Soil Biology & Biochemistry 1: 301–307.

  • Thalmann A., 1968. Zur Methodik der Bestimmung der Dehydrogenase Aktivität in Boden Mittels Triphenyltetrazoliumchlorid (TTC). Landwirtsch. Forsch., 21: 249–258.

  • Wallace R., Lochhead A., 1950. Qualitative studies of soil microorganisms IX. Amino acid requirements of rhizosphere bacteria – Canadian Journal Research, section C, 28: 1–6.

  • Van Veen J.A., Paul E.A., 1981. Organic C dynamics in grassland soils. Backround information and computer stimulation. Canadian Journal of Soil Science, 61: 185–201.


Journal + Issues