In Vitro Hypoglycemic and Radical Scavenging Activities of Certain Medicinal Plants

Thanh Sang Vo 1 , Phuong Uyen Le 1  and Dai-Hung Ngo 2
  • 1 NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam
  • 2 Faculty of Natural Sciences, Thu Dau Mot University, Binh Duong province, Vietnam

Abstract

The purpose of this study is to investigate in vitro hypoglycemic and free radical scavenging activities of some medicinal plants including Ficus glomerata (FG), Pandanus amaryllifolia (PaA), Artocarpus altilis (AA), Gomphrena celosioides (GC) and Gynostemma pentaphyllum (GP). Alpha-amylase inhibitory assay was examined by dinitrosalicylic acid reaction. Glucose up-take assay was investigated by LO-2 cell model. DPPH and ABTS+ scavenging assays were performed by spectrophotometry. Cell viability was determined by MTT method. It was found that the extracts including FG, PaA, AA, GC and GP were able to inhibit alpha-amylase activity up to 38.4 ± 4.2%, 47.8 ± 4.3%, 49.3 ± 3.5%, 40.1 ± 4.4% and 38.5 ± 3.8%, respectively. Moreover, glucose adsorption and glucose uptake capacity of these extracts were evidenced. In addition, free radical scavenging activity of these extracts was indicated in a range of 30.6-54.5% for DPPH radical and 31.8-51.1% for ABTS+ radical. Especially, these extracts exhibited no cytotoxicity effect on human hepatic LO-2 cells and human gastric BGC-823 cells at the concentration of 100 µg/ml. The results indicated that A. altilis leaves were effective in inhibiting alpha-amylase activity, increasing glucose adsorption and glucose uptake and scavenging free radicals. Therefore, it could be suggested to be a promising hypoglycemic agent for managing type 2 diabetes.

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  • 1. Hung HY, Qian K, Morris-Natschke SL, Hsu CS, Lee KH. Recent discovery of plant-derived anti-diabetic natural products. Nat Prod Rep 2012; 29: 580-606.

  • 2. Guillausseau PJ, Meas T, Virally M, Laloi-Michelin M, Médeau V, Kevorkian JP. Abnormalities in insulin secretion in type 2 diabetes mellitus. Diabetes Metab 2008; 34: S43-8.

  • 3. Medina Escobar P, Moser M, Risch L, Risch M, Nydegger UE, Stanga Z. Impaired glucose metabolism and type 2 diabetes in apparently healthy senior citizens. Swiss Med Wkly 2015; 145: w14209.

  • 4. White NH. Long-term outcomes in youth with diabetes mellitus. Pediatr Clin North Am 2015; 62: 889-909.

  • 5. Chaudhury A, Duvoor C, Reddy Dendi VS, Kraleti S, Chada A, Ravilla R, Marco A, Shekhawat NS, Montales MT, Kuriakose K, Sasapu A, Beebe A, Patil N, Musham CK, Lohani GP, Mirza W. Clinical review of antidiabetic drugs: implications for type 2 diabetes mellitus management. Front Endocrinol 2017; 8: 6.

  • 6. Mishra BB, Tiwari VK. Natural products: an evolving role in future drug discovery. Eur J Med Chem 2011; 46: 4769-807.

  • 7. Vo TS, Ngo DH, Kim SK. Marine algae as a potential pharmaceutical source for anti-allergic therapeutics. Process Biochem 2012; 47: 386-94.

  • 8. Chakraborty P. Search for new molecules/prospects of drug discovery from herbal medicines. J Complement Med Alt Healthcare 2018; 5: 1-3.

  • 9. Cragg GM, Newman DJ. Biodiversity: A continuing source of novel drug leads. Pure Appl Chem 2005; 77: 7-24.

  • 10. Eddouks M, Chattopadhyay D, De Feo V, Cho WC. Medicinal plants in the prevention and treatment of chronic diseases. Evid Based Complement Alternat Med 2012; 2012: 458274.

  • 11. Koparde AA, Doijad RC, Magdum CS. (2019). Natural Products in drug discovery. In: Perveen, S. & Al-Taweel, A (eds.). Pharmacognosy - Medicinal Plants. London, UK: IntechOpen.

  • 12. Galabuzi C, Agea JG, Fungo BL, Kamoga RMN. Traditional medicine as an alternative form of health care system: a preliminary case study of nangabo sub-county, central uganda. Afr J Tradit Complement Altern Med 2010; 7: 11-6.

  • 13. Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK. Antidiabetic agents from medicinal plants. Curr Med Chem 2006; 13: 1203-18.

  • 14. Shapiro K, Gong WC. Natural products used for diabetes. J Am Pharm Assoc 2002; 42: 217-26.

  • 15. Yatoo MI, Saxena A, Gopalakrishnan A, Alagawany M, Dhama K. Promising antidiabetic drugs, medicinal plants and herbs: an update. Int J Pharmacol 2017; 13: 732-45.

  • 16. Ahmed F, Urooj A. Traditional uses, medicinal properties, and phytopharmacology of Ficus racemosa: a review. Pharm Biol 2010; 48: 672-81.

  • 17. Cheeptham N, Towers GHN. Light-mediated activities of some Thai medicinal plant teas. Fitoterapia 2002; 73: 651-62.

  • 18. Chiabchalard A, Nooron N. Antihyperglycemic effects of Pandanus amaryllifolius Roxb. leaf extract. Pharmacogn Mag 2015; 11: 117-22.

  • 19. Naira N. Artocarpus altilis: Over view of a plant which is referred to as bread fruit. Int J Pharm Sci Res 2013; 3: 273-6.

  • 20. Monalisa M, Chinmay PA. Review on phytochemistry, bio-efficacy, medicinal and ethno-pharmaceutical importance of Artocarpus altilis. Int J Pharm Pharm Sci 2015; 3: 219-31.

  • 21. Siqueira JC. Phytogeography of Brazillian Amaranthaceae. Pesquisa Botanica 1994; 95: 5-21.

  • 22. Ilyas M, Tarnam A, Begum N. Biological potential and phytopharmacological screening of Gomphrena species. Global J Pharm 2013; 7: 457-64.

  • 23. Razmovski-Naumovski V, Huang THW, Tran VH, Li GQ, Duke CC, Roufogalis BD. Chemistry and pharmacology of Gynostemma pentaphyllum. Phytochem Rev 2005; 4: 197-219.

  • 24. Bhutkar MA, Bhise SB. In vitro assay of alpha amylase inhibitory activity of some indigenous plants. Int J Chem Sci 2012; 10: 457-62.

  • 25. Ou S, Kwok KC, Li Y, Fu L. In vitro study of possible role of dietary fiber in lowering postprandial serum glucose. J Agri Food Chem 2001; 49: 1026-9.

  • 26. van de Venter M, Roux S, Bungu LC, Louw J, Crouch NR, Grace OM, Maharaj V, Pillay P, Sewnarian P, Bhagwandin N, Folb P. Antidiabetic screening and scoring of 11 plants traditionally used in South Africa. J Ethnopharmacol 2008; 119: 81-6.

  • 27. Vo TS, Le PU, Ngo DH. The increased gamma-amino-butyric acid content by optimizing fermentation conditions of bacteria from kimchi and investigation of its biological activities. EurAsian J BioSci 2018; 12: 369-76.

  • 28. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.

  • 29. Mahmood N. A review of α-amylase inhibitors on weight loss and glycemic control in pathological state such as obesity and diabetes. Comp Clin Path 2016; 25: 1253-64.

  • 30. Rehman K, Chohan TA, Waheed I, Gilani Z, Akash MSH. Taxifolin prevents postprandial hyperglycemia by regulating the activity of α-amylase: Evidence from an in vivo and in silico studies. J Cell Biochem 2019; 120: 425-38.

  • 31. Kato E, Kushibiki N, Inagaki Y, Kurokawa M, Kawabata J. Astilbe thunbergii reduces postprandial hyperglycemia in a type 2 diabetes rat model via pancreatic alpha-amylase inhibition by highly condensed procyanidins. Biosci Biotechnol Biochem 2017; 81: 1699-705.

  • 32. Poovitha S, Parani M. In vitro and in vivo α-amylase and α-glucosidase inhibiting activities of the protein extracts from two varieties of bitter gourd (Momordica charantia L.). BMC Complement Altern Med 2016; 16: 185.

  • 33. Agarwal P, Gupta R. Alpha-amylase inhibition can treat diabetes mellitus. J Med Health Sci 2016; 5: 1-8.

  • 34. Fang W, Wei C, Dong Y, Tang X, Zu Y, Chen Q. The effect on gut microbiota structure of primarily diagnosed type 2 diabetes patients intervened by sancai lianmei particle and acarbose: a randomized controlled trial. J Clin Trials 2016; 6: 270.

  • 35. Santeusanio F, Compagnucci PA. A risk-benefit appraisal of acarbose in the management of noninsulindependent diabetes mellitus. Drug Saf 1994; 11: 432-44.

  • 36. Prabhakar PK, Doble M. Mechanism of action of natural products used in the treatment of diabetes mellitus. Chin J Integr Med 2011; 17: 563-74.

  • 37. Perry JR, Ying W. A review of physiological effects of soluble and insoluble dietary fibers. J Nutr Food Sci 2016; 6: 476.

  • 38. Schinner SS, Cherbaum WA, Bornstein SR, Barthel A. Molecular mechanisms of insulin resistance. Diabet Med 2005; 22: 674-82.

  • 39. Xia EQ, Zhu SS, He MJ, Luo F, Fu CZ, Zou TB. Marine peptides as potential agents for the management of type 2 diabetes mellitus - A prospect. Mar Drugs 2017; 15:88

  • 40. Karnieli E, Armoni M. Regulation of glucose transporters in diabetes. Horm Res 1990; 33: 99-104.

  • 41. Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress - A concise review. Saudi Pharm J 2016; 24: 547-53.

  • 42. Rahimi-Madiseh M, Malekpour-Tehrani A, Bahmani M, Rafieian-Kopaei M. The research and development on the antioxidants in prevention of diabetic complications. Asian Pac J Trop Med 2016; 9: 825-31.

  • 43. Singh R, Devi S, Gollen R. Role of free radical in atherosclerosis, diabetes and dyslipidaemia: larger-than-life. Diabetes Metab Res Rev 2015; 31: 113-26.

  • 44. Zatalia SR, Sanusi H. The role of antioxidants in the pathophysiology, complications, and management of diabetes mellitus. Acta Med Indones 2013; 45: 141-7.

  • 45. Sen T, Samanta SK. Medicinal plants, human health and biodiversity: a broad review. Adv Biochem Eng Biotechnol 2015; 147: 59-110.

  • 46. Mazid M, Khan TA, Mohammad F. Role of secondary metabolites in defense mechanisms of plants. Biol Med 2011; 3: 232-49.

  • 47. Jamil MMA, Mammam SGHB, Wahab RA. Artocarpus altilis extract effect on cervical cancer cells. Materialstory: Proceedings 2018; 5: 15559-66.

  • 48. Tsui KC, Chiang TH, Wang JS, Lin LJ, Chao WC, Chen BH, Lu JF. Flavonoids from Gynostemma pentaphyllum exhibit differential induction of cell cycle arrest in H460 and A549 cancer cells. Molecules 2014; 19: 17663-81.

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