Effects of Provinols on Cardiodynamics and Coronary Flow in Islodated Rat Hearts

Open access

Abstract

Provinols are an alcohol-free extract of red wine that contains a wide range of polyphenols. Polyphenols are a group of chemical compounds found in diverse plants. Polyphenols are considered to protect against cardiovascular disease. Although some older epidemiological studies have indicated that the positive effects of red wine on heart disease can be attributed to the alcohol content alone, there is now powerful evidence that polyphenols present in red wine are responsible for these positive effects. The hearts of male Wistar albino rats (n = 36, 12 in each experimental group, 10 weeks old, body mass 250 ± 30 g) were excised and retrogradely perfused according to the Langendorff technique at a gradually increasing perfusion pressure (40-120 cmH2O). Parameters of cardiac function (dp/dt max, dp/dt min, SLVP, DLVP, HR, CF) were measured after perfusion with three different concentrations of provinols (5 μg/ml, 10 μg/ml and 50 μg/ml). Administration of the highest dose (50 μg/ml) induced a significant increase in dp/dt max, dp/dt min, HR and CF compared with control conditions at CPP = 40 cmH2O, while an intermediate dose increased dp/dt max at the same CPP. Generally viewed, the results of the present study suggest that provinols may have a beneficial effect on the intact myocardium and coronary circulation. These findings could constitute an important step in further investigation of these polyphenols under different representative experimental conditions in the heart, as well as providing a good basis for potential clinical studies in this field.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Seruga M Novak I Jakobek L. Determination of polyphenols content and antioxidant activity of some red wines by differential pulse voltammetry HPLC and spectrophotometric methods. Food Chem. 2011; 124: 1208-16.

  • 2. Renaud S De Lorgeril M. Wine alcohol platelets and the French paradox for coronary heart disease. The Lancet. 1992; 339(8808): 1523-6.

  • 3. Hennekens CH Willett W Rosner B et al. Effects of beer wine and liquor in coronary death. JAMA. 1979; 242: 1973-4.

  • 4. Rimm EB Giovannucci E Willet WC et al. Alcohol and mortality. Lancet. 1991; 338: 1073 - 4.

  • 5. De Whalley CV Rankin SM Hoult JR Jessup W Leake DS. Flavonoids inhibit the oxidative modification of low density lipoproteins by macrophages. BiochemPharmacol. 1990; 39: 1743 -50.

  • 6. Teissedre PL Frankel EN Waterhouse AL et a1. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Sci Food Agric. 1996; 70: 55 - 61.

  • 7. Lavy A Puhrman B Markel A et al. Effect of dietary supplementation of red or white wine on human blood chemistry hematology and coagulation: favourable effect of red wine on plasma high-density lipoprotein. Ann NutrMetab. 1994; 38 (5) 287 - 94.

  • 8. Martin S Andriambeloson E Takeda K Andriantsitohaina R. Red wine polyphenols increase calcium in bovine aortic endothelial cells: a basis to elucidate signaling pathways leading to nitric oxide production. British journal of pharmacology. 2002; 135(6): 1579-87.

  • 9. Hertog MGL Feskens EJM Hollman PCH Katan MB Kroumhout D. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zupthen Elderly Study. Lancet. 1993; 342: 1007-11.

  • 10. Keli SO Hertog MGL Feskens EJM Kroumhout D. Flavonoids antioxidant vitamins and risk of stroke. The Zupten Study. Arch Intern Med. 1996; 156: 637-42.

  • 11. He S Sun C Pan Y. Red wine polyphenols for cancer prevention. Int J Mol Sci. 2008; 9(5): 842-53.

  • 12. Pietta P Simonetti P Gordana C Brusamolino A Morazzoni P Bombardelli E. Relationship between rate and extent of catechin absorption and plasma antioxidant status. BiochemMolBiol Int. 1998; 46: 895- 903.

  • 13. Pechanova O Bernátová I Babál P et al. Red wine polyphenols prevent cardiovascular alterations in L-NAMEinduced hypertension. J Hypertens.2004; 22(8): 1551-9.

  • 14. Pal S Ho N Santos C et al. Wine Polyphenolics Increase LDL Receptor Expression and Activity and Suppress the Secretion of ApoB100 from Human Hep G2 Cells. Nutr. 2003; 133 (3): 700-6.

  • 15. Zenebe W Pechánová O Andriantsitohaina R. Red wine polyphenols induce vasorelaxation by increased nitric oxide bioactivity. Physiol Res. 2003; 52(4): 425-32.

  • 16. Dillenburg DR Mostarda C Moraes-Silva IC et al. Resveratrol and grape juice differentially ameliorate cardiovascular autonomic modulation in L-NAMEtreated rats. Auton Neurosci. 2013; 179(1-2): 9-13.

  • 17. Bernátová I Pechánová O Babál P Kyselá S Stvrtina S Andriantsitohaina R. Wine polyphenols improve cardiovascular remodeling and vascular function in NO-deficient hypertension. Am J Physiol Heart Circ Physiol. 2002; 282(3): H942-8.

  • 18. Fitzpatrick DF Hirschfield SL Coffey RG. Endotheliumdependent vasorelaxing activity of wine and other grape products.Am J Physiol. 1993; 265 (2 Pt 2): H774-8.

  • 19. Pecháňová O Rezzani R Babál P Bernátová I Andriantsitohaina R. Beneficial Effects of Provinols™: Cardiovascular System and Kidney. Physiol. Res. 2006; 55 (1): S17-S30.

  • 20. Fuster V Badimon L Badimon JJ Chesebro JH. The patogenesis of coronary artery disease and the acute coronary syndromes. New Engl J Med. 1992; 326: 242-50.

  • 21. Andriambelson E Kleschyov A Muller B Beretz A Stoclet JC Andriantsitohaina R. Nitric oxide production and endothelium-dependent vasorelaxation induced by wine polyphenols in rat thoracic aorta. Br J Pharmacol. 1997; 120: 1053-8.

  • 22. Middleton EJR Kandaswami C Theoharides TC. The effect of plant flavonoids on mammalian cells: Implications for inflammation heart disease and cancer. Pharmacol Rev. 2000; 52: 673-751.

  • 23. Zenebe W Pecháňová O. Effects of red wine polyphenolic compounds on the cardiovascular system. BratislLekListy. 2002; 103: 159-65.

  • 24. Curin Y Andriantsitohaina R. Polyphenols as potential therapeutical agents against cardiovascular diseases. Pharmacol Rep. 2005; 57: 97-107.

  • 25. Perez-Vizcaino F Duarte J Andriantsitohaina R. Endothelial function and cardiovascular disease: effects of quercetin and wine polyphenols. Free radical research. 2006; 40(10): 1054-65.

  • 26. Ferrara A Fusi F Gorelli B Sgaragli G Saponara S. Effects of freeze-dried red wine on cardiac function and ECG of the Langendorff-perfused rat heart. Can J Physiol Pharmacol. 2014; 92(2): 171-4.

  • 27. Zilkens RR Burke V Hodgson JM Barden A Beilin LJ Puddey IB. Red wine and beer elevate blood pressure in normotensive men. Hypertension. 2005; 45(5): 874-9.

  • 28. Wallace CH Baczkó I Jones L Fercho M Light PE. Inhibition of cardiac voltage-gated sodium channels by grape polyphenols. Br J Pharmacol. 2006; 149(6): 657-65.

  • 29. Shimada K Watanabe H Hosoda K Takeuchi K Yoshikawa J. Effect of red wine on coronary flow-velocity reserve. Lancet 1999; 354:1002.

Search
Journal information
Impact Factor


CiteScore 2018: 0.13

SCImago Journal Rank (SJR) 2018: 0.118
Source Normalized Impact per Paper (SNIP) 2018: 0.079

Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 147 75 1
PDF Downloads 66 39 1