Being an economical and endangered species, microsatellite markers of Taxus chinensis var. mairei were very limited. We have developed a set of microsatellite markers, which was benefit for future genetic analysis of this rare species. Polymorphic loci were developed from congeneric species by cross-species amplification methods, and new primers were redesigned to test for potential null alleles. 15 loci showed polymorphism. The number of alleles per locus varied from 2 to 23 tested in 48 individuals. The observed heterozygosity (Ho) and expected heterozygosity (He) values ranged form 0.000 to 0.854 and 0.082 to 0.827, respectively. Newly redesigned primer confirmed that no null allele existed in most suspected loci. These microsatellite markers will be useful for future genetic analysis and conservation of this endangered species.
If the inline PDF is not rendering correctly, you can download the PDF file here.
ALDROCH, P. R., M. JAGTAP, C. H. MICHLER and S. J. ROMTERO (2003): Amplification of North American red oak microsatellite markers in European white oaks and Chinese chestnut. Silvae Genetica 52: 176-179.
BARBARÁ, T., C. PALMA-SILVA, G. M. PAGGI, F. BERED, M. F. FAY and C. LEXER (2007): Cross-species transfer of nuclear microsatellite markers: Potential and limitations. Molecular Ecology 16: 3759-3767.
CHUNG, S. M and J. E. STAUB (2003): The development and evaluation of consensus chloroplast primer pairs that possess highly variable sequence regions in a diverse array of plant taxa. Theoretical and Applied Genetics 107: 757-767.
DUBREUIL, M., F. SEBASTIANI, M. MAYOL, S. C. GONZALEZMARTINEZ, M. RIBA and G. G. VENDRAMIN (2008): Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L. Conservation Genetics 9: 1665-1668.
FU, L. G., N. LI and R. R. MILL (1999): Flora of China (Vol. 4). Science Press, Beijing, Missouri Botanical Garden, St. Louis.
GUO, W., W. WANG, B. ZHOU and T. ZHANG (2006): Crossspecies transferability of G. arboreum-derived ESTSSRs in the diploid species of Gossypium. Theoretical and Applied Genetics 112: 1573-1581.
HAO, D.C., B. L. HUANG and L. YANG (2008): Phylogenetic relationships of the genus Taxus inferred from chloroplast intergenic spacer and nuclear coding DNA. Biological and pharamaceutical bulletin 31: 260-265.
HEESACKER, A., V. K. KISHORE, W. X. GAO, S. X. TANG, M. K. JUDITH, G. ALAN, M. MARTA, K. ALEXANDER, M. M. RICHARD, L. ZHAO, H. R. LOREN and J. K. STEVEN (2008): SSRs and INDELs mined from the sunflower EST database: abundance, polymorphisms, and cross-taxa utility. Theoretical and Applied Genetics 117: 1021-1029.
HUANG, C. C., T. Y. CHIANG and T. W. HSU (2008): Isolation and characterization of microsatellite loci in Taxus sumatrana (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA). Conservation Genetics 9: 471-473.
JARNE, P. and P. J. LAGODA (1996): Microsatellites, from molecules to populations and back. Trends in Ecology and Evolution 11: 424-429.
KONDO, H., T. TAHIRA, H. HAYASHI, K. OSHIMA and K. HAYASHI (2000): Microsatellite genotyping of post-PCR fluorescently labeled markers. Biotechniques 29: 868-872.
LI, N. W., S. A. HE, X. C. SHU, Q. WANG and F. PENG (2010): Genetic diversity and structure analyses of wild and ex-situ conservation populations of Taxus chinensis var. mairei based on ISSR marker. Journal of Plant Resources and Environment 20: 25-30.
MAHMOODI, P., S. M. K. NEKOUEI, M. MARDI, S. M. PIRSEYEDI, M. R. GHAFFARI, M. RAMROODI and B. A. SIAHSAR (2010): Isolation and characterization of new microsatellite marker in Taxus baccata L. Conservation Genetics Resources 2: 195-199.
MIAO, Y. C., J. R. SU, Z. J. ZHANG, H. LI, J. LUO and Y. P. ZHANG (2008): Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis. Conservation Genetics 9: 1683-1685.
PEAKALL, R. and P. E. SMOUSE (2006): GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288-295.
PEAKALL, R., S. GILMORE, W. KEYS, M. MORGANTE and A. RAFALSKI (1998): Cross-species amplification of soybean (Glycinemax) simple sequence repeats (SSRs) within the genus and other legume genera: implications for the transferability of SSRs in plants. Molecular Biology and Evolution 15: 1275-1287.
PRIMMER, C. R., A. P. MØLLER and H. ELLEGREN (1996): A widerange survey of cross-species microsatellite amplification in birds. Molecular Ecology 5: 365-378.
RAYMOND, M and F. ROUSSET (1995): Genepop (Version-1.2): Population genetics software for exact tests and ecumenicism. Journal of Heredity 86: 248-249.
SQUIRRELL, J., P. M. HOLLONGSWORTH, M. WOODHEAD, J. RUSSELL, A. J. LOWE, M. GIBBY and W. POWELL (2003): How much effort is required to isolate nuclear microsatellites from plants? Molecular Ecology 12: 1339-1348.
TSUMURA, Y., K. YOSHIMURA, N. TOMARU and K. OHBA (1995): Molecular phylogeny of conifers using RFLP analysis of PCR-amplified specific chloroplast genes. Theoretical and Applied Geneticst 91: 1222-1236.
VAN OOSTERHOUT, C., W. F. HUTCHINSON, D. P. M. WILLS and P. SHIPLEY (2004): MICRO CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4: 535-538.
VINYALLONGA, S. L., J. L. ALVARADO, T. H. CONSTANTINIDIS, A. SUSANNA and N. G. JACAS (2011): Microsatellite crossspecies amplification in the genus Centaurea (Compositae). Collectanea Botanica 30: 17-27.
WEISING, K. and R. C. GARDNER (1999): A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms. Genome 42: 9-19.
YANG, J. B., H. T. LI, D. Z. LI, J. LIU and L. M. GAO (2009): Isolation and characterization of microsatellite markers in the endangered species Taxus wallichiana using the FIASCO method. HortScience 44: 2043-2045.
ZANE, L, L. BARGELLONI and T. PATARNELLO (2002): Strategies for microsatellite isolation: A review. Molecular Ecology 11: 1-16.
ZHANG, H. Y., Y. Q. CHEN and W. B. LIAO (2003): RAPD analysis of population genetic diversity of Taxus mairei. Acta Botanica Boreali-Occidentalia Sinica 23: 1994-1997.
ZHENG, W. J and L. G. FU (1978): Flora of China (Vol. 7). Science Press, Beijing.
ZHOU, Y., G. P. CHEN, Y. J. SU and Z. W. WANG (2009): Microsatellite loci from Taxus chinensis var. mairei (Taxaceae), an endangered and economically important tree species in China. Frontiers of Biology in China 4: 214-216.