Seven genomic SSR markers of Eucalyptus were developed from DNA sequences of E. grandis deposited in GenBank. Their repeat motifs were revealed by resequencing with an individual tree of E. urophylla or E. tereticornis, and five out of the seven markers turned out to be heterozygous within the specific tree sequenced. The sequence identity ranged from 75.06% to 96.66%, with an average of 87.31%. These markers could be valuable in genetics studies in Eucalyptus. This report demonstrates the advantages of re-sequencing in developing SSR markers from publicly accessible databases.