We investigated the association of inbreeding and infection by the introduced disease white pine blister rust (caused by the fungus Cronartium ribicola J. C. Fisch) with genetic diversity of whitebark pine (Pinus albicaulis Engelm.) by genetically comparing cohorts of different ages in natural stands. Isozyme analysis of bud tissue was used to estimate expected and observed heterozygosity (He and Ho), and Wright’s fixation index (Fis) for three age cohorts (seedling, young, and mature), sampled from 14 sites in British Columbia, Oregon, Idaho, and Montana. Comparison of genetic diversity parameters among cohorts within a site was used to assess the extent and persistence of inbreeding with age, while comparisons of parameters among sites within a cohort were used to assess the impact of the disease on genetic diversity. Significant evidence of inbreeding (Fis >0) was found in all age cohorts. When sites were stratified by level of blister rust infection, differences in Fis and Ho among cohorts were only significant when level of infection was low. A significant negative association was found between level of blister rust infection and Ho in the mature cohort. This suggests that when differential selection due to blister rust is weak, more heterozygous individuals may be favored; however, more homozygous individuals may have higher fitness under higher blister rust levels
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Alexander H. M. P. H. Thrall J. Antonovics A. M. Jarosz and P. V. Oudemans (1996): Population dynamics and genetics of plant disease: a case study of anthersmut disease. Ecology 77: 990–996.
Altizer S. D. Harvell and E. Friedle (2003): Rapid evolutionary dynamics and disease threats to biodiversity. Trends Ecol. Evol. 18: 589–596.
Bingham R. T. (1966): Breeding blister rust resistant western white pine. III. Comparative performance of clonal and seedling lines from rust-free selections. Silvae Genet. 15: 160–164.
Bingham R. T. A. E. Squillace and J. W. Wright (1960): Breeding blister rust resistant western white pine. II. First results of progeny tests including preliminary estimates of heritability and rate of improvement. Silvae Genet. 9: 33–41.
Bingham R. T. R. J. Hoff and G. I. McDonald (1971): Disease resistance in forest trees. Annu. Rev. Phytopathol. 9: 433–452.
Bower A. D. and S. N. Aitken (2007): Mating System and Inbreeding Depression in Whitebark Pine (Pinus albicaulis Engelm.). Tree Genet. Genomes 3: 379–388.
Burdon J. J. and J. N. Thompson (1995): Changed patterns of resistance in a population of Linum marginale attacked by the rust pathogen Melapsora lini. J. Ecol. 83: 199 206.
Burdon R. D. (2001): Genetic diversity and disease resistance: some considerations for research breeding and deployment. Can. J. For. Res. 31: 596-606.
Burdon J. J. P. H. Thrall and L. Ericson (2006): The current and future dynamics of disease in plant communities. Ann. Rev. Phytopathol. 44: 19-39.
Campbell E. M. and J. A. Antos (2000): Distribution and severity of white pine blister rust and mountain pine beetle on whitebark pine in British Columbia. Can. J. For. Res. 30: 1051–1059.
Cheliak W. M. and J. A. Pitel (1984): Techniques for starch gel electrophoresis of enzymes from forest tree species. Can. For. Serv. Petawawa Natl. For. Inst. Rep. No. Pi-X-42.
Cheliak W. M. B. P. Dancik K. Morgan F. C. Yeh and C. Strobeck (1985): Temporal variation of the mating system in a natural population of jack pine. Genetics 109: 569–584.
Chu Z. Y. Ouyang J. Zhang H. Yang and S. Wang (2004): Genome-wide analysis of defense-responsive genes in bacterial blight resistance of rice mediated by the recessive R gene xa13. Mol. Genet. Genomics 271: 111–120.
Daughtrey M. L. and C. R. Hibben (1994): Dogwood anthracnose: a new disease threatens 2 native Cornus species. Annu. Rev. Phytopathol. 32: 61–73.
Dyer R. J. and V. L. Sork (2001): Pollen pool heterogeneity in shortleaf pine Pinus echinata Mill. Mol. Ecol. 10: 859–866.
El-Kassaby Y. A. M. D. Meagher J. Parkinson and F. T. Portlock (1987): Allozyme inheritance heterozygosity and outcrossing rate among Pinus monticola near Ladysmith British Columbia. Heredity 58: 173–181.
Farris M. A. and J. B. Mitton (1984): Population density outcrossing rate and heterozygote superiority in ponderosa pine. Evolution 38: 1151–1154.
Fins L. and W. J. Libby (1982): Population variation in Sequoiadendron: seed and seedling studies vegetative propagation and isozyme variation. Silvae Genet. 31: 102–110.
Gilbert G. S. (2002): Evolutionary ecology of plant diseases in natural ecosystems. Annu. Rev. Phytopathol. 40: 13–43.
Goudet J. (1995): Fstat version 1.2: a computer program to calculate Fstatistics. J. Hered. 86: 485–486.
Hoff R. J. and G. I. McDonald (1971): Resistance to Cronartium ribicola in Pinus monticola: short shoot fungicidal reaction. Can. J. Bot. 49: 1235–1239.
Houston D. B. and D. R. Houston (2000): Allozyme genetic diversity among Fagus grandifolia trees resistant or susceptible to beech bark disease in natural populations. Can. J. For. Res. 30: 778–789.
Jorgensen S. M. and J. L. Hamrick (1997): Biogeography and population genetics of whitebark pine Pinus albicaulis. Can. J. For. Res. 27: 1574–1585.
Kim M.-S. S. J. Brunsfeld G. I. McDonald and N. B. Klopfenstein (2003): Effect of white pine blister rust (Cronartium ribicola) and rust-resistance breeding on genetic variation in western white pine (Pinus monticola). Theor. Appl. Genet. 106: 1004–1010.
Kinloch B. B. (1982): Mechanisms and inheritance of rust resistance in conifers. In: Resistance to Pests and Diseases in Forest Trees. Proceedings of the 3rd International Workshop on Genetics of Host-Parasite Interactions in Forestry Sept. 1980. Wagenigen The Netherlands.
Kinloch B. B. and G. E. Dupper (2002): Genetic specificity in the white pine-blister rust pathosystem. Phytopathology 92: 278–280.
Kinloch B. B. D. A. Davis and D. Burton (2008): Resistance and virulence interactions between two white pine species and blister rust in a 30-year field trial. Tree Genet. Genomes 4: 65–74.
Krakowski J. S. N. Aitken and Y. A. El-Kassaby (2003): Inbreeding and conservation genetics in whitebark pine. Conserv. Genet. 4: 581–593.
Krutovskii K. V. D. V. Politov and Y. P. Altukhov (1995): Isozyme study of population genetic structure mating system and phylogenetic relationships of the five stone pine species (subsection cembrae section strobi subgenus strobus). In: P. Baradat W. T. Adams and G. Muller-Starck (eds). Population genetics and genetic conservation of forest trees. SPB Academic Publishing Amsterdam The Netherlands p279.
Ledig F. T. (1991): Human impacts on genetic diversity in forest ecosystems. Oikos 63: 8–108.
Ledig F. T. (1998): Genetic variation in Pinus. In: Richardson D. M. (ed) Ecology and biogeography of Pinus. Cambridge University Press Cambridge p527.
Ledig F. T. P. D. Hodgskiss and D. R. Johnson (2006): Genetic diversity and seed production in Santa Lucia fir (Abies bracteata) a relict of the Miocene broadleaved evergreen forest. Conserv. Genet. 7: 383–398.
Ledig F. T. B. Bermejo-Velazquez P. D. Hodgskiss D. R. Johnson C. Flores-Lopez and V. Jacob-Cervantes (2000): The mating system and genetic diversity in Martinez spruce an extremely rare endemic of Mexico’s Sierra Madre Oriental: an example of facultative selfing and survival in interglacial refugia. Can. J. For. Res. 30: 1156–1164.
Little E. L. Jr. (1971): Atlas of United States trees volume 1 conifers and important hardwoods. USDA For. Serv. Misc. Publ. 1146.
Loo J. A. (2009): Ecological impacts of non-indigenous invasive fungi as forest pathogens. Biol. Invasions 11: 81–96.
Maloney P. E. S. C. Lynch S. F. Kane C. E. Jensen and D. M. Rizzo (2005): Establishment of an emerging generalist pathogen in redwood forest communities. J. Ecol. 93: 899–905.
Marquardt P. E. C. S. Echt B. K. Epperson and D. M. Pubanz (2007): Genetic structure diversity and inbreeding of eastern white pine under different management conditions. Can. J. For. Res. 37: 2652–2662.
McDonald G. I. and R. J. Hoff (1970): Resistance to Cronartium ribicola in Pinus monticola: early shedding of infected needles. USDA Forest Service Research Note INT-124. Intermountain Forest and Range Experiment Station Ogden Utah pp 8.
McDonald G. I. and D. Dekker-Robinson (1998): Long term differential expression of blister rust resistance in western white pine. Proceeding of the First IUFRO Rusts of Forest Trees Working Party Conference. Finnish Forest Resources Institute Research Paper No 712.
McDonald B. A. B. K. Bellamy J. Zhan and D. N. Appel (1998): The effect of an oak wilt epidemic on the genetic structure of a Texas live oak population. Can. J. Bot. 76: 1900–1907.
Mitton J. B. (1995): Genetics and the physiological ecology of conifers. In: Smith W. K. Hinckley T. M. (eds.) Ecophysiology of Coniferous Forests. Academic Press New York p1.
Mitton J. B. Y. B. Linhart S. W. Sturgeon and J. P. Farrar (1977): Protein polymorphisms detected in mature needle tissue of ponderosa pine. Am. J. Bot. 42: 1154–1157.
Mitton J. B. R. G. Latta and G. E. Rehfeldt (1997): The pattern of inbreeding in Washoe pine and survival of inbred progeny under optimal environmental conditions. Silvae Genet. 46: 21–219.
Mopper S. J. B. Mitton T. G. Whitham N. S. Cobb and K. M. Christensen (1991): Genetic differentiation and heterozygosity in pinyon pine assocated with resistance to herbivory and environmental stress. Evolution 45: 989–999.
Morgante M. G. G. Vendramin P. Rossi and A. M. Olivieri (1993): Selection against inbreds in early livecycle phases in Pinus leucodermis Ant. Heredity 70: 622–627.
Muona O. R. Yazdani and D. Rudin (1987): Genetic change between life stages in Pinus sylvestris: allozyme variation in seeds and planted seedlings. Silvae Genet. 36: 39–42.
Ouborg N. J. A. Biere and C. L. Mudde (2000): Inbreeding effects on resistance and transmission-related traits in the Silene-Microbotryum pathosystem. Ecology 81: 520–531.
Plessas M. E. and S. H. Strauss (1986): Allozyme differentiation among populations stands and cohorts in Monterey pine. Can. J. For. Res. 16: 1155-1164.
Politov D. V. K. V. Krutovskii and K. V. Altukhov (1992): Isozyme loci characteristics of gene banks of populations of cedar pines. Genetika 28: 76–95.
Politov D. V. M. M. Belokon and Y. S. Belokon (2006): Dynamics of allozyme heterozygosity in Siberian dwarf pine Pinus pumila (Pall.) Regel populations of the Russian far east: comparison of embryos and maternal plants. Russ. J. Genet. 42: 1127–1136.
Richardson B. A. S. J. Brunsfeld and N. B. Klopfenstein (2002): DNA from bird-dispersed seed and wind-disseminated pollen provides insights into postglacial colonization and population genetic structure of whitebark pine (Pinus albicaulis). Mol. Ecol. 11: 215–227.
SAS Institute (1999): The SAS system for windows. SAS Institute Inc. Cary North Carolina.
Selander R. K. M. H. Smith S. Y. Yang W. E. Johnson and J. R. Gentry (1971): Biochemical polymorphism and systematics in the genus Peromyscus I. Variation in the old-field mouse (Peromyscus polionotus). Stud. Genet. VI. 7103: 49–90.
Shaw D. V. and R. W. Allard (1982): Isozyme heterozygosity in adult and open-pollinated embryo samples of Douglas-fir. Silva Fenn. 16: 115–121.
Shea K. L. (1987): Effects of population structure and cone production on outcrossing rates in Engelmann spruce and subalpine fir. Evolution 41: 124–136.
Smith J. P. and J. T. Hoffman (2000): Status of white pine blister rust in the Intermountain West. West. N. Am. Naturalist 60: 165–179.
Starova N. V. Y. A. Yanbaev N. K. Yumadilov E. N. Adler V. A. Dukharev and Z. K. Shigapov (1990): Genetic variability in age groups of scotch pine. Genetika 26: 314–319.
Stilwell K. L. H. M. Wilbur C. R. Werth and D. R. Taylor (2003): Heterozygote advantage in the American Chestnut Castanea dentata (Fagaceae). Am. J. Bot. 90: 207–213.
Stuart-Smith G. J. (1998): Conservation of whitebark pine in the Canadian Rockies: blister rust and population genetics. Masters Thesis University of Alberta Edmonton.
Tomback D. F. and W. S. Schuster (1994): Genetic population structure and growth form distribution in bird-dispersed pines. In: W. C. Schmidt and F. K. Holtmeier (eds). International Workshop on Subalpine Stone Pines and Their Environment: The Status of Our Knowledge; 1992 September 5–11; St. Moritz Switzerland; Gen. Tech. Rep. INT-GTR-309. U.S. Department of Agriculture Forest Service Rocky Mountain Research Station Ogden UT. p 43.
Toyoda K. N. C. Collins A. Takahashi and K. Shirasu (2002): Resistance and susceptibility of plants to fungal pathogens. Transgenic Res. 11: 567–582.
Weir B. S. and C. C. Cockerham (1984): Estimating Fstatistics for the analysis of population structure. Evolution 38: 1358–1370.
Weisberg S. (1985): Applied Linear Regression. John Wiley & Sons New York. 324 pp.
Williams C. G. and O. Savolainen (1996): Inbreeding depression in conifers: Implications for breeding strategy. Forest Sci. 42: 102–117.
Wright S. (1951): Evolution and the genetics of populations. Vol. II. University of Chicago Press Chicago IL.
Yandell U. G. (1992): An allozyme analysis of whitebark pine (Pinus albicaulis Engl.). Masters Thesis University of Nevada Reno.
Zeglen S. (2002): Whitebark pine and white pine blister rust in British Columbia Canada. Can. J. For. Res. 32: 1265–1274.