Population Genetic Structure of Laurus nobilis L. Inferred From Transferred Nuclear Microsatellites

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Abstract

Species with fragmented populations and low population size often display low within-population genetic diversity and strong among-population differentiation. Laurus nobilis L. (Lauraceae), common laurel, has a scattered distribution throughout the Mediterranean, with only few autochthonous populations. Our goal was to elucidate if this species has range-wide genetic structure and if planted material can be traced back to its origin. Genetic diversity was investigated using 4 polymorphic nuclear microsatellites (nSSR) transferred from two species of Lauraceae. Sixty-six laurel trees were selected from 7 widely separated populations within the Mediterranean distribution area of the species. A total of 34 alleles (9 alleles per locus on average) were found. Mean genetic diversity within-population (Hs), was 0.558. Genetic differentiation among populations (GST = 0.243) was high compared to that of other angiosperms. Laurus nobilis can be separated into two main gene pools, one from western (Tunisia, Algeria and France) and the other from eastern Mediterranean (Turkey). The Algerian, Tunisian and French populations presented a strong genetic similarity, compatible with the fact that North African laurel populations could be recently introduced from north-western Mediterranean stock.

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  • ARROYO-GARCIA R. J. M. MARTINEZ-ZAPATER J. A. FERNANDEZ PRIETO and R. ALVAREZ-ARBESU (2001): AFLP evaluation of genetic similarity among laurel populations. Euphytica 122 155-164.

  • BARBERO M. and P. QUEZEL (1994): Place rôle et valeur historique des éléments laurifoliés dans les végétations préforestières et forestières ouest-méditerranéennes. Annales di Botanica 52 81-133.

  • ECHT C. S. D. DEEMER T. KBISIAK and C. D. NELSON (2006): Microsatellites for Lindera species. Molecular Ecology Notes 6 1171-1173.

  • FADY B. (2005): Is there really more biodiversity in Mediterranean forest ecosystems? Taxon 54 (4) 905-910.

  • FANG F. S. SANG K. Y. CHEN A. GOSSLAU C. HO and R. T. ROSEN (2005): Isolation and identification of cytotoxic compounds from Bay leaf (Laurus nobilis). Food Chemistry 93 497-501.

  • FARWIG N. C. BRAUN and K. BOEHNING-GAESE (2008): Human disturbance reduces genetic diversity of an endangered tropical tree Prunus africana (Rosaceae). Conservation genetics 9 (2) 317-326.

  • GLAUBITZ J. C. L. C. EMEBIRI and G. F. MORAN (2001): Dinucleotide microsatellites from Eucalyptus sieberi: inheritance diversity and improved scoring of singlebase differences. Genome 44 1041-1045.

  • GOUDET J. (1995): Fstat version 1.2. A computer program to calculate F-statistics. Journal of Heredity 86 485-486.

  • HANSSON B. and L. WESTERBERG (2002): On the correlation between heterozygosity and fitness in natural populations. Molecular Ecology 11 (12) 2467-2474.

  • JARNE P. and P. J. L. LAGODA (1996): Microsatellites from molecules to populations and back. Trends in Ecology and Evolution 11: 424-430.

  • KAWATA M. (1997): Loss of genetic variability in a fragmented continuously distributed population. Population Ecology 39 (2) 227-237.

  • LACERDA A. E. BD. M. KANASHIRO and A. M. SEBBENN (2008): Effects of reduced impact logging on genetic diversity and spatial genetic structure of a Hymenaea courbaril population in the Brazilian Amazon Forest. Forest Ecology and Management 255 (3/4) 1034-1043.

  • LEDIG F. T. R. P. GURIES and B. A. BONEFELD (1983): The relation of growth to heterozygosity in pitch pine. Evolution 37 (6) 227-1238.

  • LINNAEUS C. (1753): Ray Society. Species Plantarum. Facsimile of the first edition of 1753 published by Bernard Quaritch Ltd. in 1857.

  • MATSUDA H. H. SHIMODA K. NINOMIYA and M. YOSHIKAWA (2002): Inhibitory mechanism of costunolide a sesquiterpene lactone isolated from Laurus nobilis on blood-ethanol elevation in rats: involvement of inhibition of gastric emptying and increase in gastric juice secretion. Alcohol and Alcoholism 37 121-127.

  • NEI M. (1987): Molecular evolutionary genetics. Columbia University Press New York

  • ODDOU-MURATORIO S. G. G. VENDRAMIN J. BUITEVELD and B. FADY (2009): Population estimators or progeny tests: what is the best method to assess null allele frequencies at SSR loci? Conservation Genetics online First.

  • PEAKALL R. and P. E. SMOUSE (2006): GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6 288-295.

  • PECH B. and J. BRUNETON (1982): Alcaloïdes du laurier noble Laurus nobilis. Journal of Natural Product 45 560-563.

  • PETIT R. J. J. DUMINIL S. FINESCHI A. HAMPE D. SALVINI and G. G. VENDRAMIN (2005): Comparative organization of chloroplast mitochondrial and nuclear diversity in plant populations. Molecular Ecology 14 689-701.

  • QIU Y. L. J. LEE F. BERNASCONI-QUADRONI D. E. SOLTIS P. S. SOLTIS M. ZANIS E. A. ZIMMER Z. CHEN V. SAVOLAINEN and M. W. CHASE (1999): The earliest angiosperms: evidence from mitochondrial plastid and nuclear genomes. Nature 402 404-407.

  • QUÉZEL P. and F. MÉDAIL (2003): Ecologie et biogéographie des forêts du bassin méditerranéen. Elsevier (Collection Environnement) Paris 573 p.

  • RODRÍGUEZ-SÁNCHEZ F. B. GUZMÁN A. VALIDO P. VARGAS and J. ARROYO (2009): Late Neogene history of the laurel tree (Laurus L. Lauraceae) based on phylogeographical analyses of Mediterranean and Macaronesian populations. Journal of Biogeography online first.

  • SAYAH M. J. VALIZADEH and M. KAMALINEJAD (2002): Anticonvulsant activity of the leaf essential oil of Laurus nobilis against pentylenetetrazole- and maximal electroshock- induced seizures. Phytomedicine 9 212-216.

  • SCHNELL R. J. J. S. BROWN C. T. OLANO E. J. POWER and C. A. KROL (2003): Evaluation of avocado germplasm using microsatellite markers. The American Society for Horticultural Science 128 (6) 881-889.

  • SHARON D. P. B. CREGAN S. MHAMEED M. KUSHARSKA J. HILLEL E. LAHAV and U. LAVI (1997): An integrated genetic linkage map of avocado. Theoretical Applied Genetics 95 911-921.

  • SOTO A. Z. LORENZO and L. GIL (2003): Nuclear microsatellite markers for the identification of Quercus ilex L. and Q. suber L. hybrides. Silvae Genetica 52 (2) 63-66.

  • VAN OOSTERHOUT C. W. F. HUTCHINSON D. P. M. WILLS and P. SHIPLEY (2004): MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4 535-538.

  • VALBUENA-CARABANA M. S. C. GONZALEZ-MARTINEZ and L. GIL (2008): Coppice forests and genetic diversity: A case study in Quercus pyrenaica Willd. from Central Spain. Forest Ecology and Management 254 225-232.

  • WEISING K. and R. C. GARDNER (1999): A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms. Genome 42 9-19.

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