Heterogeneity and Spatial Autocorrelation for Chloroplast Haplotypes in Three Old Growth Populations of Northern Red Oak


In eastern North America, evidence for cryptic northern refugia could contribute to resolving Reid’s Paradox, the disparity between the rate of oak recolonization indicated by pollen deposition and the rate indicated by contemporary seed dispersal studies. Severe anthropogenic disturbance of oak-dominated forests throughout eastern North America followed by regeneration from isolated patches and deliberate planting in some regions could obscure the signal of discontinuity expected from small, cryptic refugia. In this study of northern red oak, Quercus rubra L., the dominant representative of Quercus section Lobatae in the eastern United States, we address the question of appropriate sample size for accurate detection of the biogeographical distribution of chloroplast haplotype diversity in Q. rubra. We examined chloroplast DNA (cpDNA) variation in all Q. rubra over 17 cm in diameter (310 trees) in three forest fragments with documented histories of minimal disturbance for the last 100-190 years. cpDNA polymorphisms in three intergenic regions revealed different haplotype frequencies between the two local fragments located within 1 km of each other and complete discontinuity for the predominant haplotype between these two sites and a site 207 km distant. Haplotypes displayed positive spatial autocorrelation over 10-40 meter distances. Sample sizes of 10 or fewer taken at 50 meter intervals along a linear transect yielded poor estimates of haplotype frequencies and did not accurately detect haplotype richness.

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  • ALDRICH, P. R., G. R. PARKER, J. ROMERO-SEVERSON and C. H. MICHLER (2005): Confirmation of recruitment failure of red oaks in Indiana old-growth forest. Forest Science 51: 406-416.

  • BAKER, R. G., E. A. BETTIS III, R. F. DENNISTON, L. A. GONZALEZ, L. E. STRICKLAND et al. (2002): Holocene Paleoenvironments in Southeastern Minnesota - Chasing the Prairie-Forest Ecotone. Palaeogeography, Palaeoclimatology, Palaeoecology 177: 103-122.

  • CHESSER, R. K. and R. J. BAKER (1996): Effective Sizes and Dynamics of Uniparentally and Diparentally Inherited Genes. Genetics 144: 1225-1235.

  • DAVIES, S., A. WHITE and A. LOWE (2004): An investigation into effects of long-distance seed dispersal on organelle population genetic structure and colonization rate: a model analysis. Heredity 93: 566-576.

  • DELCOURT, P. A. and H. R. DELCOURT (1987): Long Term Forest Dynamics of the Temperate Zone: A Case Study of Late-Quaternary Forests in Eastern North America, pp. 246-252 in Ecological Studies, Analysis and Synthesis, edited by W. D. BILLINGS, F. GOLLEY, O. L. LANGE and J. S. OLSEN. Springer-Verlag, New York.

  • FRALISH, J. S., F. B. CROOKS, J. L. CHAMBERS and F. M. HARTY (1991): Comparison of Presettlement, Secondgrowth and Old-growth Forest on Six Site Types in the Illinois Shawnee Hills. American Midland Naturalist 125: 294.

  • JACKSON, S. T., R. S. WEBB, K. H. ANDERSON, J. T. OVERPECK and T. WEBB et al. (2000): Vegetation and environment in Eastern North America during the Last Glacial Maximum. Quaternary Science Reviews 19: 489-508.

  • JOHNSON, W. C., C. S. ADKISSON, T. R. CROW and M. D. DIXON (1997): Nut caching by blue jays (Cyanocitta cristata L.): Implications for tree demography. American Midland Naturalist 138: 357-370.

  • JOHNSON, W. C. and T. WEBB (1989): The Role of Blue Jays (Cyanocitta-Cristata L.) in the Postglacial Dispersal of Fagaceous Trees in Eastern North-America. Journal of Biogeography 16: 561-571.

  • MAGNI, C. R., A. DUCOUSSO, H. CARON, R. J. PETIT and A. KREMER (2005): Chloroplast DNA variation of Quercus rubra L. in North America and comparison with other Fagaceae. Molecular ecology 14: 513-524.

  • PARKER, G. R., D. J. LEOPOLD and J. K. EICHENBERGER (1985): Tree Dynamics in an Old-Growth, Deciduous Forest. Forest Ecology and Management 11: 31-57.

  • PETIT, R. J., S. BREWER, S. BORDACS, K. BURG, R. CHEDDADI et al. (2002): Identification of refugia and postglacial colonisation routes of European white oaks based on chloroplast DNA and fossil pollen evidence. Forest ecology and management 156: 49-74.

  • PONS, O., and R. J. PETIT (1995): Estimation, Variance and Optimal Sampling of Gene Diversity. 1. Haploid Locus. Theoretical and Applied Genetics 90: 462-470.

  • ROMERO-SEVERSON, J., P. ALDRICH, Y. FENG, W. L. SUN and C. MICHLER (2003): Chloroplast DNA variation of northern red oak (Quercus rubra L.) in Indiana. 26: 43-49.

  • SCHLARBAUM, S. E., R. P. ADAMS, W. T. BAGLEY and W. J. WAYNE (1982): Post-glacial migration pathwaya of Quercus rubra L., Northern Red Oaks, as indicated by regional genetic-variation patterns. Silvae genetica 31: 150-158.

  • SCHMELZ, D. V. and A. A. LINDSEY (1965): Size class structure of old growth forests in Indiana. Forest Science 11: 258-264.

  • SMALLWOOD, P. D., M. STEEL, A., E. RIBBENS and W. J. MCSHEA (1998): Detecting the effect of seed hoarders on the distribution of seedlings of tree species: Gray squirrels (Sciurus carolinensis) and oaks (Quercus) as a model system: In: pp. 211-222 in Ecology and evolutionary biology of tree squirrels. Virginia Museum of Natural History, Martinsville, Virginia.

  • STEELE, M. A., G. TURNER, P. D. SMALLWOOD, J. O. WOLFF and J. RADILLO (2001): Cache management by small mammals: Experimental evidence for the significance of acorn-embryo excision. Journal of mammalogy 82: 35-42.


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