Resistance of Castanea Clones to Phytophthora Cinnamomi: Testing and Genetic Control

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Summary

The resistance of chestnut clones to Phytophthora cinnamomi was evaluated by a soil inoculation experiment under controlled environmental conditions, as well as by excised and intact stem tests. One-year-old plants of fifty different clones were inoculated with two isolates of Phytophthora cinnamomi and evaluated fourteen weeks after inoculation. There were significant differences among clones for all root and collar rot variables. There were significant differences for isolates of P. cinnamomi but only for the collar rot variables. A total of 84% of plants grown in infested soil showed symptoms of root rot but only 50% of the plants with root rot, showed also had collar rot. The roots of resistant clones were able to confine the colonization, in roots and from roots to collar. Percentage circumference of collar rot was the best indicator or descriptor of sensitivity, a 50% of clones were resistant or highly resistant clones, with respectively less than 20% and than 10% circumference of collar rot. Percentage of survival of plants is not sufficient to indicate resistance to the pathogen, as mortality may be affected by environmental conditions or by other pathogens. The clonal heritability of collar rot variables ranged between 0.54 and 0.71. The plants grown on inoculated soil showed a reduction in growth. The phenotypic and genotypic correlations between soil infestation characteristics and the length of necrosis in both intact and excised stem tests were positive and highly significant and indicated similar resistance rankings of the clones.

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  • ALDWINCKLE H. S. F. J. POLACH W. T. MOLIN and R. C. PEARSON (1975): Pathogenicity of Phytophthora cactorum isolates from New York apple trees and other sources. Phytopathology 65: 989-994.

  • BENNET I. J. (1988): Use of tissue culture techniques for the improvement of Eucalyptus marginata Donn. ex Sm. Ph. D. thesis. Murdoch University Perth Australia.

  • BOLETÍN OFICIAL DEL ESTADO (BOE) (2003): RD 289/2003 de 7 de marzo sobre comercialización de los materiales forestales de reproducción. BOE núm. 58 8/3/2003.

  • BRASIER C. M. (1969): The effect of light and temperature on reproduction in vitro in two tropical species of Phytophthora. Transactions of the British Mycological Society 52: 105-113.

  • BRIDGEWATER F. (1992): Mating Desings. In: FINS et al. (eds.) Handbook of Quantitative Forest Genetics. 69-95. 1992 Kluwer Academic Publishers. Netherlands.

  • BROWNE G. T. and S. M. MIRCETICH (1988): Effects of flood duration on the development of Phytophthora root and crown rots of apple. Phytopathology 78(6): 846-851.

  • BROWNE G. T. and S. M. MIRCETICH (1993): Relative resistance of thirteen apple rootstocks to three species of Phytophthora. Phytopatology 83: 744-749.

  • BURDON R. D. (1977): Genetic correlation as a concept for studying genotype-environment interaction in forest tree breeding. Silvae Genetica 26: 168-175.

  • CAHILL D. M. I. J. BENNETT and J. A. MCCOMB (1993): Mechanisms of resistance to Phytophthora cinnamomi in clonal micropropagated Eucalyptus marginata. Plant Pathology 42: 865-872.

  • DAVISON E. M. M. C. STUKELY C. E. CRANE and F. C. S. TAY (1994): Invasion of Phloem and Xylem of Woody Stems and Roots of Eucalyptus marginata and Pinus radiata by Phytophthora cinnamomi. Phytophatology 84 (4): 335-340.

  • DIXON K.W. and K. THINLAY SIVASITHAMPARAM (1984): Technique for rapid assessment of tolerance of Banksia spp. to root rot caused by Phytophthora cinnamomi. Plant Disease 68 1077-80.

  • EGEL D. S. (1999): Preventing seedling diseases in the greenhouse. Purdue University Cooperative Extensio service. http://www.ces.purdue.edu/extmedia/menu.htm.

  • ERWIN D. C. and O. K. RIBEIRO (1996): Phytophthora Diseases Worldwide. American Phytopathological Society Press St Paul MN.

  • EUROPEAN COUNCIL (1999): Directive 199/150/EC on the marketing of forest reproductive material.

  • FALCONER D. S. (1989): Introduction to quantitative genetics. Third edition. Longman Scientific and Technical.

  • FERNÁNDEZ-LÓPEZ J. (1996): Variabilidad isoenzimática morfológica y selección clonal en Castanea sativa Miller Castanea crenata Sieb. et Zucc. Castanea mollissima e híbridos interespecíficos. Tesis doctoral Univ. Polit. de Madrid.

  • FERNÁNDEZ-LÓPEZ J. R. A. VÁZQUEZ-RUIZ-DE-OCENDA R. DÍAZ-VÁZQUEZ and S. PEREIRA-LORENZO (2001): For. Snow Landsc. Res. Evaluation of resistance of Castanea sp. clones to Phytophthora sp. using excised chestnut shoots 76(3): 451-454.

  • HARRIS J. A. F. Y. KASSABY I. W. SMITH and G. C. MARKS (1983): Intra-specific variation in resistance of Phytophthora cinnamomi in Eucalyptus regnans. Aust. Plant Pathol. 12: 20-22.

  • HÜBERLI D. G. E. St J. HARDY I. C. TOMMERUP and I. J. COLQUHOUN (1998): Temperature changes resistance of clonal Eucalyptus marginata to Phytophthora cinnamomi. In Seventh International Congress of Plant Pathology: 2.5.4. Edinburgh.

  • HÜBERLI D. I. C. TOMMERUP M. P. DOBROWOLSKI M. C. CALVER and G. E. St J. HARDY (2001): Phenotypic variation in a clonal lineage of two Phytophthora cinnamomi populations from Western Australia. Mycol. Res 105 (9): 1053-1064.

  • MIRANDA-FONTAÍÑA M. E. and J. FERNÁNDEZ-LÓPEZ (2005): Variabilidad clonal de castaño híbrido en resistencia a Phytophthora cinnamomi. IV Congreso Forestal Español. Zaragoza. 26-30 Septiembre 2005. Mesa temática 2 ponencia 4.

  • MIRANDA-FONTAÍÑA M. E. FERNÁNDEZ-LÓPEZ J. VETTRAINO A. M. VANNINI A. 2006. Variabilidad genética en resistencia a Phytophthora cinnamomi de 51 clones de Castanea sp. evaluada mediante test de tallo cortado. Accepted for publication in: Investigación Agraria. Sistemas y Recursos Forestales.

  • RAMOS GUEDES-LAFARGUE M. and G. SALESSES (1999): Ink disease resistance: some preliminary elements from the study of different crosses. Proceedings of the 2nd Intern. Symp. on Chestnut Bordeaux France 19-23 October 1998. Acta Horticulturae 464: 355-361.

  • ROBIN C. and M. L. DESPREZ-LOUSTAU (1998): Testing variability in pathogenicity of Phytophthora cinnamomi. European Journal of Plant Pathology 104 465-75.

  • ROBIN C. G. CAPRON and M. L. DESPREZ-LOUSTAU (2001): Root infection by Phytophthora cinnamomi in seedlings of three oak species. Plant Pathology 50: 708-716.

  • SALESSES G. L. RONCO J. E. CHAUVIN and J. CHAPA (1993a): Amélioration génétique du chataignier Mise au point de tests d’evaluation du comportement vis-à-vis de la maladie de léncre. Lárboriculture Fruitiere 458: 23-31.

  • SALESSES G. J. CHAPA and P. CHAZERANS (1993b): Screening and breeding for ink disease resistance. Proceedings of the International Congress on Chestnut Spoleto Italy October 0-23 545-549.

  • SANTINI A. G. P. BARZANTI and P. CAPERTTI (2003): Susceptibility of some Mesophilic Hardwoods to Alder Phytophthora. Journal of Phytopathology 151 (7-8) 406-410.

  • SCHAD C. G. SOLIGNAT J. GRENTE and P. VENOT (1952): Recherches sur le chataignier a la Station de Brive. Annales de l’amelioration des plantes III: 369-458.

  • STUKELY M. J. C. and C. E. CRANE (1994): Genetically based resistance of Eucalyptus marginata to Phytophthora cinnamomi. Phytopathology 84: 650-656.

  • THEMANN V. K. and S. WERRES (1998): Use of Rhododendron leaves to detect Phytophthora species in root and soil samples. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes 50 37-45.

  • THEMANN K. and S. WERRES: Guidelines for the handling of the Rhododendron leaf test to detect Phytophthora spp. in root soil and water samples. www.bba.de/phytoph/diagn_r.htm

  • TIPPETT J. T. T. C. HILL and B. L. SHEARER (1985): Resistance of Eucalyptus spp. to invasion by Phytophthora cinnamomi. Australian Journal of Botany 33: 409-18.

  • URQUIJO P. (1956): La regeneración del castaño. Bol. Pat. Veg. Ent. Agr. XXII 217-232.

  • VANNINI A. and A. M. VETTRAINO (2002): Sources of resistance to Phytophthora cambivora in naturalized and cultivated proveniences of sweet chestnut in Italy and Spain. JPP. Volume 84 (3) - November 2002.

  • VETTRAINO A. M. A. SCALISE S. CHERUBINI and A. VANNINI (2001): Variability in resistance to Phytophthora cambivora of Castanea sativa wild population and selected cultivars in Italy and Spain. Second International IUFRO meeting on Phytophthora in forests and natural ecosystems. 30 Sept-5 Oct in Perth and Albany Western Australia.

  • VETTRAINO A. M G. NATILI N. ANSELMI and A. VANNINI (2001): Recovery and pathogenicity of Phytophthora species associated with a resurgence of ink disease in Castanea sativa in Italy. Plant Pathology 50 90-96.

  • VETTRAINO A. M. O. MOREL C. PERLEROU S. ROBIN S. DIAMANDIS and A. VANNINI (2005): Occurrence and distribution of Phytophthora species in European chestnut stands and their association with Ink Disease and crown decline. European Journal of Plant Pathology 111: 169-180 (12).

  • VIEITEZ E. (1960): Obtención de castaños resistentes a la enfermedad de la tinta. Centro Regional de Enseñanzas y Experiencias Forestales de Lourizán Pontevedra.

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