Faster Evaluation of Induced Floral Sterilit

H. Hoenicka 1 , O. Nowitzki 1 , Th. Debener 2 ,  and Matthias Fladung 1
  • 1 Federal Research Centre for Forestry and Forest Products, Institute for Forest Genetics and Forest Tree Breeding, Sieker Landstr. 2, D-22927 , Grosshansdorf, Germany
  • 2 University of Hanover, Institute for Plant Genetics, Herrenhaeuser Str. 2, D-30419 , Hannover, Germany


A major concern over the use of transgenic trees is the potential for transgene dispersal through pollen and seeds. The incorporation of sterility inducing genes into transgenic lines of trees has been proposed to reduce or even avoid gene flow of transgenes into non-transgenic interbreeding species. The evaluation of strategies for the induction of sterility in transgenic forest tree species has been hindered by their long vegetative phases. In this study an early flowering 35S::Leafy poplar line was used for the faster evaluation of the sterility construct C-GPDHC::Vst1. The combination of two transgenic approaches, one to induce early flowering and a second for the induction of sterility, allowed evaluation of this sterility strategy two years after transformation. This is a very short period of time considering the long vegetative period of seven to twenty years common in forest tree species. This approach opens opportunities for the assessment of sterility mechanisms for this plant group.

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  • AHN, J. H., D. MILLER, V. J. WINTER, M. J. BANFIELD, J. H. LEE, S. Y. YOO, S. R. HENZ, R. L. BRADY and D. WEIGEL (2006): A divergent external loop confers antagonistic activity on floral regulators FT and TFL1. EMBO J. 25: 605-14.

  • BEALS, T. P. and R. B. GOLDBERG (1997): A novel cell ablation strategy blocks tobacco anther dehiscence. Plant Cell 9: 1527-45.

  • BÖHLENIUS, H., T. HUANG, L. CHARBONNEL-CAMPAA, A. M. BRUNNER, S. JANSSON, S. H. STRAUSS and O. NILSSON (2006): CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees. Science 312: 1040-3.

  • CHIANG, C. H., J. J. WANG, F. J. JAN, S. D. YEH and D. GONSALVES (2001): Comparative reactions of recombinant papaya ringspot viruses with chimeric coat protein (CP) genes and wild-type viruses on CP-transgenic papaya. J. Gen. Virol. 82: 2827-36.

  • CONFALONIERI, M., A. BALESTRAZZI, S. BISOFFI and D. CARBONERA (2003): In vitro culture and genetic engineering of Populus spp.: synergy for forest tree improvement. Plant Cell Tissue and Organ Culture 72: 109-138.

  • DOYLE, J. J. and J. L. DOYLE (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.

  • ELLSTRAN, N. C. (2001): When transgenes wander, should we worry? Plant Physiol. 125: 1543-5.

  • ENDO, T., T. SHIMADA, H. FUJII, Y. KOBAYASHI, T. ARAKI and M. OMURA (2005): Ectopic expression of an FT homolog from citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.). Transgenic Res. 14: 703-12.

  • FLADUNG, M. (2003): Holzbiologische, -chemische, technologische und phytopathologische Untersuchungen an rolC-transgenen Hybridaspen (P. tremula L. x P. tremula L x P. tremuloides Michx.) aus einem Freisetzungsversuch. BFH-Mitteilungen, Hamburg.

  • FLADUNG, M. and M. R. AHUJA (1995): Sandwich method for non-radioactive hybridisations. Biotechniques 18: 3-5.

  • FLADUNG, M., K. GROSSMANN and M. R. AHUJA (1997): Alterations in hormonal and developmental characteristics in transgenic Populus conditioned by the rolC gene from Agrobacterium rhizogenes. J. Plant Physiol. 150: 420-427.

  • FLADUNG, M. (1999): Gene stability in transgenic aspen- Populus. I. Flanking DNA sequences and T-DNA structure. Mol. Gen. Genet. 260: 574-581.

  • FLADUNG, M. and S. KUMAR (2002): Gene stability in transgenic aspen-Populus. III. T-DNA repeats influence transgene expression differentially among different transgenic lines. Plant Biology 4: 329-338.

  • FLADUNG, M., M. KALDORF, W. GIEFFERS, B. ZIEGENHAGEN, H. J. MUHS and S. KUMAR (2004): Field analysis of transgenic aspen. In: WALTER, C., CARSON, M. (ed). Plantation forest biotechnology for the 21st century, Research Signpost, Kerala, India, pp 393-403.

  • GONSALVES, D. (1998): Control of papaya rinspot virus in papaya: a case study. Ann. Rev. of Phytopathol. 36: 415-437.

  • GUILLEY, H., R. K. DUDLEY, G. JONARD, E. BALAZS and K. E. RICHARDS (1982): Transcription of Cauliflower mosaic virus DNA: detection of promoter sequences, and characterization of transcripts. Cell 30: 763-73.

  • HACKETT, R. M., M. J. LAWRENCE and C. H. FRANKLIN (1992): A Brassica S-locus related gene promoter directs expression in both pollen and pistil of tobacco. Plant J. 2: 613-617.

  • HAIN, R., H. J. REIF, E. KRAUSE, R. LANGEBARTELS, H. KINDL, B. VORNAM, W. WIESE, E. SCHMELZER, P. H. SCHREIER and R. H. STOCKER (1993): Disease resistance results from foreign phytoalexin expression in a novel plant. Nature 361: 153-6.

  • HARTLEY, R. W. (1988): Barnase and barstar. Expression of its cloned inhibitor permits expression of a cloned ribonuclease. J. Mol. Biol. 202: 913-5.

  • HAUSMANN, L. and R. TÖPFER (1999): Entwicklung der Plasmid-Vektoren. Vortr. Pflanzenzüchtung 45: 155-172.

  • HÖFIG, K. P., R.L. MOYLE, J. PUTTERILL and C. WALTER (2003): Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis. Planta. 217: 858-67.

  • HÖFIG, K. P., R. MÖLLER, L. DONALDSON and J. C. PUTTERILL (2006): Towards male sterility in Pinus radiata - a stilbene synthase approach to genetically engineer nuclear male sterility. Plant Biotechnology J. 4: 333-343.

  • HOENICKA, H. and M. FLADUNG (2006a): Biosafety in Populus spp. and other forest trees: from non-native species to taxa derived from traditional breeding and genetic engineering. Trees 20: 131-144.

  • HOENICKA, H. and M. FLADUNG (2006b): Genome instability in plants derived from genetic engineering, pp. 301-321. In: Tree Transgenesis, edited by M. FLADUNG and D. EWALD, Springer Verlag, Germany.

  • HOOD, E. E., S. B. GELVIN, L. S. MELCHERS and A. HOEKEMA (1993): New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Research 2: 208-218.

  • HSU, C. Y., Y. LIU, D. S. LUTHE and C. YUCEER (2006): Poplar FT2 Shortens the Juvenile Phase and Promotes Seasonal Flowering. Plant Cell. 18: 1-16.

  • HUANG, T., H. BOLENIUS, S. ERIKSSON, F. PARCY and O. NILSSON (2005): The mRNA of the Arabidopsis gene FT moves from leaf to shoot apex and induces flowering. Science 309: 1694-6.

  • KOLTUNOW, A. M, J. TRUETTNER, K. H. COX, M. WALLROTH and R. B. GOLDBERG (1990): Different Temporal and Spatial Gene Expression Patterns Occur during Anther Development. Plant Cell. 2: 1201-1224.

  • KUMAR, S. and M. FLADUNG (2001): Gene stability in transgenic aspen (Populus). II. Molecular characterization of variable expression of transgene in wild and hybrid aspen. Planta 213: 731-740.

  • KUVSHINOV, V., K. KOIVU, A. KANERVA and E. PEHU (2001): Molecular control of transgene escape from genetically modified plants. Plant Sci. 160: 517-522.

  • LANNENPAA, M., M. HASSINEN, A. RANKI, M. HOLTTA-VUORI, J. LEMMETYINEN, K. KEINONEN and T. SOPANEN (2005): Prevention of flower development in birch and other plants using a BpFULL1::BARNASE construct. Plant Cell Rep. 24: 69-78.

  • LEMMETYINEN, J., K. KEINONEN and T. SOPANEN (2004): Prevention of the flowering of a tree, silver birch. Molecular Breeding 13: 243-249.

  • LIDA, W., H. YIFAN and H. JIANJUN (2003): Transgenic forest trees for insect resistance, pp 243-261. In: Molecular Genetics and Breeding of Forest Trees, edited by S. KUMAR and M. FLADUNG, The Haworth Press, Binghamton/ USA.

  • LLOYD, G. and B. MCCOWN (1980): Combined Proceedings of the International Plant Propagators Society. 30: 421-427. MA, H. (2005): Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants. Annu Rev Plant Biol. 56: 393-434.

  • MEILAN, R. (1997): Floral induction in woody angiosperms. New Forests 14: 179-202.

  • MEILAN, R., A. BRUNNER, J. SKINNER and S. STRAUSS (2001): Modification of flowering in transgenic trees, pp. 247-256. In: Molecular Breeding of Woody Plants, edited by A. KOMAMINE and N. MOROHOSHI, Elsevier Science BV, Amsterdam/The Netherlands.

  • MEYER, P. (1995): Gene silencing in higher plants and related phenomena in other eukaryotes. Berlin, Germany: Springer Verlag.

  • MIKKELSEN, T. R., B. ANDERSEN and R. B. JORGENSEN (1996): The risk of crop transgene spread. Nature 380: 31.

  • PALMITER, R. D., R. R. BEHRINGER, C. J. QUAIFE, F. MAXWELL, I. H. MAXWELL and R. L. BRINSTER (1987): Cell lineage ablation in transgenic mice by cell-specific expression of a toxin gene. Cell 50: 435-43.

  • PASZKOWSKI, J. (1994): Homologous recombination and gene silencing in plants. Kluwer Academic Publishers, Dordrecht, The Netherlands.

  • PENA, L., M. MARTIN-TRILLO, J. JUAREZ, J. A. PINA, L. NAVARRO and J. M. MARTINEZ-ZAPATER (2001): Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time. Nat. Biotechnol. 19: 263-7.

  • ROGER, P., E. HELLENS, A. EDWARDS, N. R. LEYLAND, S. BEAN and P. M. MULLINEAUX (2000): “pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation”. Plant Mol. Biol. 42: 819-832.

  • ROTTMANN, W. H., R. MEILAN, L. A. SHEPPARD, A. M. BRUNNER, J. S. SKINNER, C. MA, S. CHENG, L. JOUANIN, G. PILATE and S. H. STRAUSS (2000): Diverse effects of overexpression of LEAFY and PTLF, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis. Plant J. 22: 235-45.

  • SKINNER, J. S., R. MEILAN, A. M. BRUNNER and S. H. STRAUSS (2000): Options for genetic engineering of floral sterility in forest treees, pp. 135-153. In: Molecular Biology of Woody Plants, edited by S. M. JAIN and S. C. MINOCHA, vol. 1, Kluwer Academic publishers, Dordrecht/The Netherlands.

  • TZFIRA, T., C. S. JENSEN, W. WANG, A. ZUCKER, B. VINOCUR, A. ALTMAN and A. VAINSTEIN (1997): Transgenic Populus tremula: a step-by-step protocol for its Agrobacteriummediated transformation. Plant Mol. Biol. Rep. 15: 219-235.

  • WALDEN, A. R., C. WALTER and R. GARDNER (1999): Genes expressed in Pinus radiata male cones include homologous to anther specific and pathogenesis response genes. Plant Physiology 121: 1103-116.

  • WALTER, C. (2004): Genetic Engineering in conifer forestry: technical and social considerations. In Vitro Cell Developmental Biology-Plant 40: 434-441.

  • WANG, H., H. M. WU and A.Y. CHEUNG (1993): Development and Pollination Regulated Accumulation and Glycosylation of a Stylar Transmitting Tissue-Specific Proline- Rich Protein. Plant Cell 5: 1639-1650.

  • WEIGEL, D. and O. NILSSON (1995): A developmental switch sufficient for flower initiation in diverse plants. Nature 377: 495-500.

  • WIGGE, P. A., M. C. KIM, K. E. JAEGER, W. BUSCH, M. SCHMID, J. U. LOHMANN and D. WEIGEL (2005): Integration of spatial and temporal information during floral induction in Arabidopsis. Science 309: 1056-1059.

  • YOO, S. K., K. S. CHUNG, J. KIM, J. H. LEE, S. M. HONG, S. Y. YOO, J. S. LEE and J. H. AHN (2005): CONSTANS activates SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 through FLOWERING LOCUS T to promote flowering in Arabidopsis. Plant Physiol. 139: 770-8.


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