Identification of Atractylodes japonica and A. macrocephala by RAPD analysis and SCAR Markers

Open access

Abstract

The Chinese plant, “Packchul”, (Atractylodes japonica or A. macrocephala), is a very important Chinese medicinal herb plant and is called Sabju in Korea. The levels of the active components are different between these two species, but these medicines are sold in Korean herbal markets without discrimination. This study was carried out to develop a method that could be used to discriminate between A. japonica and A. macrocephala based on molecular markers. To discriminate between A. japonica and A. macrocephala, RAPD analyses were used to develop SCAR markers. Eighteen species-specific RAPD bands were obtained from 52 OPERON and URP primer sets. Two SCAR markers were developed from these RAPD clones. Both SCAR markers were cloned into pGEM-T-Easy vectors and then subjected to nucleotide sequence analysis and designated AjR1 (1,117 bp) and AmR1 (1,325 bp). These two markers were sufficient to discriminate between samples of A. japonica and A. macrocephala.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • ALTSCHUL F. S. L. THOMAS A. A. MADDEN SCHAFFER Z. JINGHUI Z. ZHENG M. WEB and D. J. LIPMAN (1997): “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs” Nucleic Acids Res. 25: 3389-3402.

  • ARDIEL G. S. T. S. GREWAL P. DEBERDT B. G. ROSSNAGEL and G. J. SCOLES (2002): Inheritance of resistance to covered smut in barley and development of a tightly linked SCAR marker. Theor. Appl. Genet. 104: 457-464.

  • BANG K. H. D. H. KOO M. K. HUH N. S. SEONG and J.W. BANG (2003): Physical mapping of the species-specific RAPD fragments and teromere sequence repeats between Atractylodes japonica and Atractylodes macrocephala. Korean J. Genet. 26: 77-81.

  • BARRET P. R. DELOURME N. FOISSET and M. RENARD (1998): Development of a SCAR marker for molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus L. Theor. Appl. Genet. 97: 828-833.

  • BEHURA S. K. S. C. SAHU S. RAJAMANI A. DEVI R. MAGO S. NAIR and M. MOHAN (1999): Differentiation of Asian rice gall midge Orseolia oryzae (Wood-mason) biotypes by sequence characterized amplified regions (SCARs). Int. Mol. Biol. 8: 391-397.

  • DEDRYVER F. M. F. JUBIER J. THOUVENIN and H. GOYEAU (1996): Molecular markers linked to the leaf rust resistance gene Lr24 in different shear cultivar. Genome 39: 830-835.

  • DENG Z. S. HUANG S. XIAO and F. G. GMITTER (1997): Development and characterization of SCAR markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata. Genome 40: 697-704.

  • DOYLE J. J. and J. L. DOYLE (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11-15.

  • HERNANDEZ P. A. MARTIN and G. DORADO (1999): Development of SCARs by direct sequencing of RAPD products: a practical tool for the introgression and marker-assisted selection of wheat. Mol. Breed. 5: 245-253.

  • KIM J. H. H. JOUNG H. W. KIM and Y. P. LIM (1998): Estimation of genetic variation and relationship in potato (Solanum Tuberosum L.) cultivars using AFLP markers. Am. J. Potato Res. 75: 107-112.

  • LAHOGUE F. P. THIS and A. BONQUET (1998): Identification of a codominant SCAR marker linked to the seedlessness character in grapevine. Theor. Appl. Genet. 97: 950-959.

  • LANG N. T. P. K. SUBUDHI S. S. VIRMANI D. S. BRAR G. S. KHUSH Z. LI and N. HUANG (1999): Development of PCR based markers for thermosensitive genetic male sterility gene tms3(t) in rice (Oryza sativa L.). Hereditas 131: 121-127.

  • NAQVI N. I. and B. B. CHATTOO (1996): Development of a sequence characterized amplified region (SCAR) based indirect selection method for a dominant blast-resistance gene in rice. Genome 39: 26-30.

  • NEGI M. S. M. DEVIC M. DELSENY and M. LAKSHMIKUMARAN (2000): Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection. Theor. Appl. Genet. 101: 146-152.

  • NEI M. (1973): Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70: 3321-3323.

  • NICESE F. P. J. I. HORMAZA and G. H. MCGRANAHAN (1998): Molecular characterization and genetic relatedness among walnut (Juglans regia L.) genotypes based on RAPD markers. Euphytica 101: 199-206.

  • PARAN I. and R.W. MICHELMORE (1993): Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 85: 985-993.

  • SAMBROOK J. R. MANIATIS and E. F. FRITSCH (1989): Molecular cloning; A laboratory manual. Cold Spring Harbor New York.

  • SAKAMOTO S. H. KUDO S. SUZUKI S. SASSA S. YOSHIMURA T. NAKAYAMA M. MAEMURA T. MITAMURA Z. QI X. D. LIU Y. YAGISHITA and A. ASAI (1996): Pharmacotherapeutic effects of toki-shakuyaku-san on leukorrhagia in young women. Am. J. Chin. Med. 24: 165-168.

  • WILLIAMS J. G. K. A. R. KUBELIK K. J. LIVAK J. A. RAFALSKI and S.V. TINGEY (1990): DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res. 18: 6531-6535.

Search
Journal information
Impact Factor


IMPACT FACTOR 2018: 0,741
5-year IMPACT FACTOR: 0,651

CiteScore 2018: 0.77

SCImago Journal Rank (SJR) 2018: 0.345
Source Normalized Impact per Paper (SNIP) 2018: 0.362

Cited By
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 125 85 10
PDF Downloads 81 66 9