Žinčica is a popular Slovak dairy product made from ewes’ milk. It is a by-product resulting during ewes’ lump cheese processing. Microbiota in Žinčica have rarely been studied, especially enterococci; however, they can produce beneficial substances – bacteriocins. In this study, the presence of Enterocins (Ents) genes were analysed in enterococci from Žinčica and partially also the inhibition activity. Samples of Žinčica were collected from different agrofarms producing ewes’ lump cheese (34) in Central Slovakia. In the enterococci tested, Ent P gene was the most frequently detected (in 6 out of 7 enterococci), followed by Ent A and Ent L50B genes. Ent B gene was detected only in E. faecium 30E1. On the other hand, E. faecalis 31E2 did not contain Ent genes, although it showed inhibition activity against the indicator strains Enterococcus avium EA5, Staphylococcus aureus SA5, Listeria monocytogenes CCM4699 (inhibition zone sizing up to 20 mm). E. faecium 30E1 contained genes of four Ents; however, it showed no inhibition activity. Growth of the four indicators was inhibited due to the antimicrobial activity of E. faecium 32E1 with Ent P gene detection. This is the first study reporting on the occurrence of Ent genes in enterococci from Žinčica.
If the inline PDF is not rendering correctly, you can download the PDF file here.
Alatoom AA Cunningham SA Ihde S Mandrekar J Patel R (2011): Comparison of direct colony method versus extraction method for identification of Gram-positive cocci by use of Bruker Biotyper matrix-assissted laser desorption ionization-time of flight mass spectrometry. Journal of Clinical Microbiology 49 2868–2873.
Aymerich T Holo H Havarstein LS Hugas M Garriga M Nes IF (1996): Biochemical and genetic characterization of enterocin A from Enterococcus faecium a new antilisterial bacteriocin in the pediocin family of bacteriocins. Applied and Environmental Microbiology 62 1676–1682.
Baele M Chiers K Devriese LA Smith HE Wisselink HJ Vaneechoutte M Haesebrouck F (2001): The Gram-positive tonsillar and nasal flora of piglets before and after weaning. Journal of Applied Microbiology 91 997–1003. doi: 10.1046/j.1365-2672.2001.01463.x.
Casaus P Nilsen T Cintas LM Nes LF Hernandez PE Holo H (1997): Enterocin B a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A. Microbiology 143 2287–2294. doi: 10.1099/00221287-143-7-228-X.
Cintas LM Casaus P Havarstain LS Hernandez PE Nes IF (1997): Biochemical and genetic characterization of enterocin P a novel sec-dependent bacteriocin from Enterococcus faecium P1 with a broad antimicrobial spectrum. Applied and Environmental Microbiology 43 4231–4330. doi: 0099-2240/97S04.00+0.
Cintas LM Casaus P Holo H Havarstain LS Hernandez PE Nes IF (1998): Enterocins L50A and L50B two novel bacteriocins from Enterococcus faecium L50 are related to staphylococcal haemolysins. Journal of Bacteriology 180 1988–1994.
Euzeby JP (1997): List of bacterial names with standing in nomenclature: A folder available on the Internet. International Journal of Bacteriology 64 590–592. doi: 10.1099/00207713-47-2-590.
Franz CHMAP van Belkum MJ Holzapfel WH Abriuel H Galvez A (2007): Diversity of enterococcal bacteriocins and their grouping in a new classification scheme. FEMS Microbiology Review 31 293–310. doi: 10.1007/s12602-009-9020.y.
Franz CHM Huch M Abriouel H Holzapfel W Galvez A (2011): Enterococci as probiotics and their implication in food safety. International Journal of Food Microbiology 151125–140. doi: 10.1016/j.ijfoodmicro.2011.08.014.
Giraffa G (2003): Functionality of enterococci in dairy products. International Journal of Food Microbiology 88 215–222. doi: 10.1016/S0168-1605(03)00183-1.
Herian K (2014): Benefit of sheep milk products to human health. Milk Letters (Mlékářské Listy) 143 1–6. (in Slovak)
Kolosta M Slottova A Droncovsky M Klapacova L Kmet V Bujnakova D Laukova A Greif G Greifova M Tomaska M (2014): Characterization of lactobacilli from ewes’ and goats’ milk for their further processing re-utilisation. Potravinárstvo – Scientific Journal for Food Industry 8 130–134. doi: 10.5219/434.
Laukova A Czikkova S (2001): Antagonistic effect of enterocin CCM4231 from Enterococcus faecium on ‘bryndza’ a traditional Slovak dairy product from sheep milk. Microbiological Research 156 31–34. doi: 10.1078/0944-5013-00078.
Laukova A Strompfova V (2016): Cow mastitis milk as a source of bacteriocin active enterococci. International Journal of Biology Pharmacy and Allied Sciences (IJBPAS) 5 1185–1194.
Laukova A Marekova M Javorsky P (1993): Detection and antimicrobial spectrum of a bacteriocin-like substances produced by Enterococcus faecium CCM 4231. Letters in Applied Microbiology 16 257–260. doi: 10.1111/j.1472-765X.1993.tb01413.x.
Laukova A Vlaemynck G Czikkova S (2001): Effect of enterocin CCM4231 on Listeria monocytogenes in Saint-Paulin cheese. Folia Microbiologica 46 157–160.
Laukova A Strompfova V Szaboova R Kmet V Tomaska M (2012): Bioactive Enterococcus durans strains isolated from sheep lump cheese. Slovenský veterinársky časopis 37 277–278. (in Slovak)
Laukova A Strompfova V Szaboova R Slottova A Tomaska M Kmet V Kolosta M (2016): Bioactive enterococci isolated from Slovak ewes lump cheese. Scientia Agriculture Bohemica 47 187–193. doi: 10.1515/sab-2016-0027.
Laukova A Kandricakova A Bino E Tomaska M Kolosta M Kmet V Strompfova V (2019): Some safety aspects of enterococci from Slovak lactic acid dairy product ‘Žinčica’. Folia Microbiologica. doi: 10.1007/s12223-019-00703-5.
Marekova M Laukova A De Vuyst L Skaugen M Nes IF (2003): Partial characterization of bacteriocins produced by environmental strain Enterococcus faecium EK13. Journal of Applied Microbiology 94 523–530. doi: 10.1007/S12602.
Markova J Markvartova M (2017): Isolation and identification of bacteriocin-producing Enterococci from cheeses and sourdoughs. Mlékařské Listy 163 28 5–8. (in Czech)
Qin X Singh KV Weinstock GM (2001): Characterization of fsr a regulator controlling expression of gelatinase and serine protease in Enterococcus faecalis OG1RF. Journal of Bacteriology 183 3372–3382. doi: 10.1021/jf5006269.
Skalka B Pillich J Pospisil L (1983): Further observation on Corynebacterium renale as an indicator organism in the detection of exfoliation-positive strains of Staphylococcus aureus. Zentralblatt fuer Bacteriology and Hygiene A256 168–174.
Strompfova V Laukova A Simonova M Marcinakova M (2008): Occurrence of the structural enterocin A P B L50B genes in enterococci of different origin. Veterinary Microbiology 132 293–301. doi: 10.1016/j.vetmic.2008.05.001.
Woodford N Egelton MC Morrison D (1997): Comparison of PCR with phenotypic methods for the speciation of enterococci. Advance and Experimental Medicine 418 405–408.