Analysis of Cutaneous Microbiota of Piglets with Hereditary Melanoma

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Malignant melanoma may be a life-threatening disease caused by various conditions. Cutaneous bacteria could play a role in melanoma development or regression. The present work aimed to analyze the bacterial species present on the epidermis of piglets with hereditary melanoma. Bacteria isolated by swabs directly from melanomas and healthy epidermis were analysed using MALDI-TOF mass spectrometry. From the total of 290 isolates, 92 were identified, while the extraction by ethanol turned out to be more efficient compared to identification by direct transfer. Staphylococcus sciuri, Staphylococcus cohnii, and Lactococcus lactis were significantly more frequent on healthy skin, whereas Staphylococcus chromogenes, Staphylococcus hyicus, and Enterococcus faecalis have thrived significantly better on melanoma. Overall, the results indicate that the micro-biota of melanoma is different from that of healthy epidermis, so piglet skin bacteria inspections are recommended.

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  • Andresen LO (2005): Production of exfoliative toxin by isolates of Staphylococcus hyicus from different countries. Veterinary Record 157 376–378.

  • Andresen LO Ahrens P Daugaard L Bille-Hansen V (2005): Exudative epidermitis in pigs caused by toxigenic Staphylococcus chromogenes. Veterinary microbiology 105 291–300. doi: 10.1016/j.vetmic.2004.12.006.

  • Bataille V de Vries E (2008): Melanoma – Part 1: epidemiology risk factors and prevention. BMJ 337: a2249.

  • Brand A Singer K Koehl GE Kolitzus M Schoenhammer G Thiel A Matos C Bruss C Klobuch S Peter K Kastenberger M Bogdan C Schleicher U Mackensen A Ullrich E Fichtner-Feigl S Kesselring R Mack M Ritter U Schmid M Blank C Dettmer K Oefner PJ Hoffmann P Walenta S Geissler EK Pouyssegur J Villunger A Steven A Seliger B Schreml S Haferkamp S Kohl E Karrer S Berneburg M Herr W Mueller-Klieser W Renner K Kreutz M (2016): LDHA-Associated lactic acid production blunts tumor immunosurveillance by T and NK cells. Cell Metabolism 24 657–671. doi: 10.1016/j.cmet.2016.08.011.

  • Cervinkova M Kucerova P Cizkova J (2017): Spontaneous regression of malignant melanoma – is it based on the interplay between host immune system and melanoma antigens? Anticancer Drugs 28 819–830. doi: 10.1097/CAD.0000000000000526.

  • Chen S Wang Y Chen F Yang H Gan M Zheng SJ (2007): A highly pathogenic strain of Staphylococcus sciuri caused fatal exudative epidermitis in piglets. PLoS ONE 2 e147. doi: 10.1371/journal.pone.0000147.

  • Coates R Moran J Horsburgh MJ (2014): Staphylococci: colonizers and pathogens of human skin. Future Microbiology 9 75–91. doi: 10.2217/fmb.13.145.

  • Devriese LA Baele M Vaneechoutte M Martel A Haesebrouck F (2002): Identification and antimicrobial susceptibility of Staphylococcus chromogenes isolates from intramammary infections of dairy cows. Veterinary Microbiology 87 175–182. doi: 10.1016/S0378-1135(02)00047-0.

  • Enger BD Fox LK Gay JM Johnson KA (2015): Reduction of teat skin mastitis pathogen loads: differences between strains dips and contact times. Journal of Dairy Science 98 1354–1361. doi: 10.3168/jds.2014-8622.

  • Garza-Gonzalez E Morfin-Otero R Martinez-Vazquez MA Gonzalez-Diaz E Gonzalez-Santiago O Rodriguez-Noriega E (2011): Microbiological and molecular characterization of human clinical isolates of Staphylococcus cohniiStaphylococcus hominis and Staphylococcus sciuri. Scandinavian Journal of Infectious Diseases 43 930–936. doi: 10.3109/00365548.2011.598873.

  • Grivennikov SI Wang K Mucida D Stewart CA Schnabl B Jauch D Taniguchi K Yu G-Y Osterreicher CH Hung KE Datz C Feng Y Fearon ER Oukka M Tessarollo L Coppola V Yarovinsky F Cheroutre H Eckmann L Trinchieri G Karin M (2012): Adenoma-linked barrier defects and microbial products drive IL-23/IL-17-mediated tumour growth. Nature 491 254–258. doi: 10.1038/nature11465.

  • Hor YY Liong MT (2014): Use of extracellular extracts of lactic acid bacteria and bifidobacteria for the inhibition of dermatological pathogen Staphylococcus aureus. Dermatologica Sinica 32 141–147. doi: 10.1016/j.dsi.2014.03.001.

  • Horak V Fortyn K Hruban V Klaudy J (1999): Hereditary melanoblastoma in miniature pigs and its successful therapy by devitalization technique. Cellular and Molecular Biology (Noisy-le-Grand France) 45 1119—1129.

  • Hoste E Arwert EN Lal R South AP Salas-Alanis JC Murrell DF Donati G Watt FM (2015): Innate sensing of microbial products promotes wound-induced skin cancer. Nature Communications 6: 5932. doi: 10.1038/ncomms6932.

  • Hwang IY Lim SK Ku HO Park CK Jung SC Park YH Nam HM (2011): Occurrence of virulence determinants in fecal Enterococcus faecalis isolated from pigs and chickens in Korea. Journal of Microbiology and Biotechnology 21 1352–1355.

  • Kloos WE Zimmerman RJ Smith RF (1976): Preliminary studies on the characterization and distribution of Staphylococcus and Micrococcus species on animal skin. Applied and Environmental Microbiology 31 53–59.

  • Little EG Eide MJ (2012): Update on the current state of melanoma incidence. Dermatologic Clinics 30 355–361. doi: 10.1016/j.det.2012.04.001.

  • McCourt C Dolan O Gormley G (2014): Malignant melanoma: a pictorial review. The Ulster Medical Journal 83 103–110.

  • McIntyre MK Peacock TJ Akers KS Burmeister DM (2016): Initial characterization of the pig skin bacteriome and its effect on in vitro models of wound healing. PLoS ONE 11 e0166176. doi: 10.1371/journal.pone.0166176.

  • Nallapareddy SR Murray BE (2008): Role of serum a biological cue in adherence of Enterococcus faecalis to extracellular matrix proteins collagen fibrinogen and fibronectin. The Journal of Infectious Diseases 197 1728–1736. doi: 10.1086/588143.

  • Nemeghaire S Argudin MA Fessler AT Hauschild T Schwarz S Butaye P (2014): The ecological importance of the Staphylococcus sciuri species group as a reservoir for resistance and virulence genes. Veterinary Microbiology 171 342–356. doi: 10.1016/j.vetmic.2014.02.005.

  • Park J Friendship RM Poljak Z Weese JS Dewey CE (2013): An investigation of exudative epidermitis (greasy pig disease) and antimicrobial resistance patterns of Staphylococcus hyicus and Staphylococcus aureus isolated from clinical cases. The Canadian Veterinary Journal 54 139–144.

  • Sato H Hirose K Terauchi R Abe S Moromizato I Kurokawa S Maehara N (2004): Purification and characterization of a novel Staphylococcus chromogenes exfoliative toxin. Journal of Veterinary Medicine Series B 51 116–122. doi: 10.1111/j.1439-0450.2004.00743.x.

  • Schulthess B Brodner K Bloemberg GV Zbinden R Bottger EC Hombach M (2013): Identification of Gram-positive cocci by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry: comparison of different preparation methods and implementation of a practical algorithm for routine diagnostics. Journal of Clinical Microbiology 51 1834–1840. doi: 10.1128/JCM.02654-12.

  • Schwabe RF Jobin C (2013): The microbiome and cancer. Nature Reviews Cancer 13 800–812. doi: 10.1038/nrc3610.

  • Skalka B (1991): Occurrence of staphylococcal species in clinically healthy domestic animals. Veterinarni medicina 36 9–19. (in Czech)

  • Tran T Burt D Eapen L Keller OR (2013): Spontaneous regression of metastatic melanoma after inoculation with tetanus–diphtheria–pertussis vaccine. Current Oncology 20 e270-3. doi: 10.3747/co.20.1212.

  • Usuki A Ohashi A Sato H Ochiai Y Ichihashi M Funasaka Y (2003): The inhibitory effect of glycolic acid and lactic acid on melanin synthesis in melanoma cells. Experimental Dermatology 12 (Suppl. 2) 43–50.

  • Wilson DA Young S Timm K Novak-Weekley S Marlowe EM Madisen N Lillie JL Ledeboer NA Smith R Hyke J Griego-Fullbright C Jim P Granato PA Faron ML Cumpio J Buchan BW Procop GW (2017): Multicenter evaluation of the Bruker MALDI Biotyper CA System for the identification of clinically important bacteria and yeasts. American Journal of Clinical Pathology 147 623–631. doi: 10.1093/ajcp/aqw225.

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