The diversity and genetic relationships in 173 sacha inchi samples were analyzed using ISSR markers. Thirty ISSR primers were used, only 8 showed variability in tested samples. ISSR fragments ranged from 200 to 2500 bp. The mean number of bands per primer was 12 and the average number of polymorphic bands per primer was 11. The lowest percentages of polymorphic bands (27%), gene diversity (0.103), and Shannon’s information index (0.15) were exhibited by the Santa Lucia population, which was also geographically most distant. This fact may be attributed to a very small size of this group. In contrast, the Dos de Mayo population exhibited the highest percentage of polymorphic bands (78%), and the Santa Cruz population the highest Nei’s gene diversity index (0.238) and Shannon’s information index (0.357). The obtained level of genetic variability was 36% among tested populations and 64% within populations. Although the diversity indices were low, a cluster analysis revealed 8 clusters containing mainly samples belonging to individual populations. Principal coordinate analysis clearly distinguished Chumbaquihui, Pucallpa, Dos de Mayo, and Aguas de Oro populations, the others were intermixed. The obtained results indicated the level of genetic diversity present in this location of Peru, although it is influenced by anthropological aspects and independent on the geographical distances.
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Aliyu OM Awopetu JA (2007): Assessment of genetic diversity in three populations of cashew (Anacardium occidentale L.) using protein-isoenzyme-electrophoretic analysis. Genetic Resources and Crop Evolution 54 1489–1497. doi: 10.1007/s10722-006-9138-9.
Alvarado CT (2008): Collection identification taxonomic determination and analysis of concentration of fatty acids of the sacha inchi ecotypes in the Amazonas region. Peru-biodiverso Project. Lima Peru. (in Spanish)
Arévalo G (1995): Cultivation of sacha inchi (Plukenetia volubilis L.) in Amazon. National Programme of Research in Genetic Resources and Biotechnology – PRONARGEB Experimental Station El Porvenir Tarapoto. (in Spanish)
Beebee TJC Rowe G (2008): An introduction to molecular ecology. Oxford University Press New York.
Bekele A Feyissa T Tesfaye K (2014): Genetic diversity of anchote (Coccinia abyssinica (Lam.) Cong.) from Ethiopia as revealed by ISSR markers. Genetic Resources and Crop Evolution 61 707–719.
Bondiolli P Della Bella L Rettke P (2006): Alpha linolenic acid rich oils: composition of Plukenetia volubilis (Sacha Inchi) oil from Peru. Rivista Italiana delle Sostanze Grasse 123 120–123.
Bussmann RW Téllez C Glenn A (2009): Plukenetia huayllabambana sp. nov. (Euphorbiaceae) from the Upper Amazon of Peru. Nordic Journal of Botany 27 313–315. doi: 10.1111/j.1756-1051.2009.00460.x.
Bussmann RW Zambrana NP Téllez C (2013): Plukenetia carolis-vegae (Euphorbiaceae) – a new useful species from Northern Peru. Economic Botany 67 387–392. doi: 10.1007/s12231-013-9247-2.
Corazón-Guivin M Castro-Ruiz D Chota-Macuyama W Rodríguez A Cachique D Manco E Del-Castillo D Renno JF García-Dávila C (2008): Genetic characterization of accessions from Sacha Inchi National Germplasm Bank in San Martin region. Folia Amazónia 18 23–31. (in Spanish)
Dostálek T Münzbergová Z Plačková I (2014): High genetic diversity in isolated populations of Thesium ebracteatum at the edge of its distribution range. Conservation Genetics 15 75–86. doi: 10.1007/s10592-013-0522-7.
Doyle JJ Doyle JL (1987): A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19 11-15.
Fisher M Husi R Prati D Peintinger M van Kleunen M Schmid B (2000): RAPD variation among and within small and large populations of the rare clonal plant Ranunculus reptans (Ranunculaceae). American Journal of Botany 87 1128–1137. doi: 10.2307/2656649.
Flores D (2010): Data base of Perubiodiverso project. Bibliographic information about history and traditional uses of 3 selected plants. Biocomercioperu Project. Lima Peru. (in Spanish)
Guillen MD Ruiz A Cabo N Chirinos R Pascual G (2003): Characterization of Sacha Inchi (Plukenetia volubilis L.) oil by FTIR spectroscopy and 1H NMR. Comparison with linseed oil. Journal of the American Oil Chemists’ Society 80 755–762. doi: 10.1007/s11746-003-0768-z.
Gupta S Srivastava M Mishra GP Naik PK Chauhan RS Tiwari SK Kumar M Singh R (2008): Analogy of ISSR and RAPD markers for comparative analysis of genetic diversity among different Jatropha curcas genotypes. African Journal of Biotechnology 7 4230–4243.
Hadian J Bigdeloo M Nazeri V Khadivi-Khub A (2014): Assessment of genetic and chemical variability in Thymus caramanicus. Molecular Biology Reports 41 3201–3210. doi: 10.1007/s11033-014-3180-z.
Hamaker BR Valles C Gilman R Hardmeier RM Clark D García HH Gonzales AE Kohlstad I Castro M (1992): Amino acid and fatty acid profiles of the Inca peanut (Plukenetia volubilis L.). Cereal Chemistry 69 461–463.
Hartl DL Clark AC (eds) (2007): Inbreeding population subdivision and migration. In: Principles of population genetics. Sinauer Associates Inc. Publishers Sunderland USA 257.
Heywood VH Iriondo JM (2003): Plant conservation: old problems new perspectives. Biological Conservation 113 321–335. doi: 10.1016/S0006-3207(03)00121-6.
Nei M (1973): Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America 70 3321–3323. doi: 10.1073/pnas.70.12.3321.
Nybon H (2004): Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology 13 1143–1155.
Rao ES Kadirvel P Symonds RC Geethanjali S Ebert AW (2012): Using SSR markers to map genetic diversity and population structure of Solanum pimpinellifolium for development of a core collection. Plant Genetic Resources 10 38–48. doi: 10.1017/S1479262111000955.
Rodrigues HS Borem de Oliveira A Gomes Lopes MT Cruz CD Chaves FCM da Silva Bentes JL (2013): Genetic diversity of Sacha Inchi accessions detected by AFLP molecular markers. Revista de Ciencias Agrárias/Amazonian Journal of Agricultural and Environmental Sciences 56 55–60. doi: 10.4322/rca.2013.081. (in Portuguese)
Rodríguez Á Corazón-Guivin M Cachique D Mejía K Del Castillo D Renno J-F García-Dávila C (2010): Morphologic differentiation and ISSR (Inter Simple Sequence Repeats) in genus Plukenetia (Euphorbiaceae) in Peruvian Amazon: a new species proposal. Peruvian Journal of Biology 17 325–330. (in Spanish)
Sathe SK Hamaker BR Sze-Tao KWC Venkatachalam M (2002): Isolation purification and biochemical characterization of a novel water soluble protein from Inca peanut (Plukenetia volubilis L.). Journal of Agricultural and Food Chemistry 50 4906–4908. doi: 10.1021/jf020126a.
Shilpha J Silambarasan T Pandian SK Ramesh M (2013): Assessment of genetic diversity in Solanum trilobatum L. an important medicinal plant from South India using RADP and ISSR markers. Genetic Resources and Crop Evolution 60 807–818.