Salinity Stress Tolerance Of Camelina Investigated In Vitro

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The ability of Camelina sativa to withstand salinity stress in vitro by adding NaCl (0, 25, 50, 75, 100, 125, 150, 175, 200mM) in Murashige and Skoog basal medium was studied. Performance of the plants was measured in terms of various growth parameters and physiological and biochemical tests performed on fully grown plants. The germination capacity, cotyledon unfolding and first true leaf emergence was reduced by 30.6, 17.3, and 28.8%, respectively in 200mM salt treatment with respect to control. The plant height, relative water content, and plant water content were decreased by 85.4, 10.8, and 9.8%, respectively, in stressed plants with respect to control. A decrease in chlorophyll a and b and total chlorophyll contents (by 81.3%), as well as of protein content was registered. Electrical conductivity increased by 52.8% in stressed plants over control, as expected. Other stress indicators like guiacol peroxidase activity and malondialdehyde also increased with respect to control. At salt concentrations lower than 200mM, no clear cut retardation effects were seen. Thus, the present study opens up the scope of further assessment of survivability of camelina in salt contaminated soils.


= ascorbate peroxidase


= chlorophyll stability index


= dry weight


= electrical conductivity


= fresh weight


= germination percentage


= guiacol peroxidase


= hydrogen peroxide


= malondialdehyde


= Murashige and Skoog


= hydroxyl radical


= photosystem II


= relative humidity


= reactive oxygen species


= relative water content


= superoxide dismutase


= thiobarbituric acid


= trichloroacetic acid


= turgid weight


  • Agarwal A, Pant T, Ahmed Z (2010): Camelina sativa: a new crop with bio-fuel potential introduced in India. Current Science, 99, 1195.

  • Aghaleh M, Niknam V, Ebrahimzadeh H, Razavi K (2009): Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. euro-paea. Biologia Plantarum, 53, 243–248.

  • Ahmad P, Prasad MNV (2012): Abiotic stress responses in plants: metabolism, productivity and sustainability. Springer-Verlag, New York.

  • Ahmad S, Khan NI, Iqbal MZ, Hussain A, Hassan M (2002): Salt tolerance of cotton (Gossypium hirsutum L.). Asian Journal of Plant Sciences, 1, 715–719.

  • Ali Y, Aslam Z, Ashraf MY, Tahir GR (2004): Effect of salinity on chlorophyll concentration, leaf area, yield and yield components of rice genotypes grown under saline environment. International Journal of Engineering Science and Technology, 1, 221–225.

  • Arnon DI (1949): Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1–15.

  • Ashraf M, Ahmad S (2000): Influence of sodium chloride on ion accumulation, yield components and fibre characteristics in salt-tolerant and salt-sensitive line of cotton (Gossypium hirsutum L.). Field Crops Research, 66, 115–127.

  • Ashraf M, Harris PJC (2004): Potential biochemical indicators of salinity tolerance in plants. Plant Science, 166, 3–16.

  • Ayers R, Westcot W (1985): Water quality for agriculture. FAO Irrigation and Drainage Paper No. 29, FAO, Rome.

  • Azza M, Fatma AM, EL-Quensi EM, Farahat MM (2007): Responses of ornamental plants and woody trees to salinity. World Journal of Agricultural Sciences, 3(3), 386-395.

  • Barrs HD, Weatherley PE (1962): A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences, 15, 413–428.

  • Batool A, Ashraf M, Akram NA, Al-Qurainy F (2013): Salt-induced changes in growth, some key physio-biochemical attributes, activities of enzymatic and levels of non-enzymatic antioxidants in cauliflower (Brassica oleracea L.). The Journal of Horticultural Science and Biotechnology, 88, 231–241.

  • Bolarian M, Fernandez F, Cruz V, Cuartero J (1991): Salinity tolerance in four wild tomato species using vegetative yield-salinity response curves. The Journal of American Society of Horticultural Sciences, 116, 286–290.

  • Bradford MM (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248–254.

  • Brouwer C, Goffeau A, Heibloem M (1985): Irrigation water management: Training manual No. 1 – Introduction to irrigation. FAO Corporate Document Repository, 13/03/2001.

  • Epstein E (1980): Responses of plants to saline environments. In: DW Rains, RC Valentine, A Hollaender, eds: Genetic Engineering of Osmoregulation. Plenum Press, New York, 7-21.

  • Garg G (2010): In vitro screening of Catharanthus Roseus L. cultivars for salt tolerance using physiological parameters. International Journal of Environmental Science and Development, 1, 24–30.

  • Ghoulam C, Foursy A, Fares K (2002): Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environmental and Experimental Botany, 47, 39–50.

  • Grattan S, Grieve C (1998): Salinity–mineral nutrient relations in horticultural crops. Scientia Horticulturae, 78, 127–157.

  • Haleem A, Mohammed MA (2007): Physiological aspects of mungbean plant (Vigna radiata L. Wilczek) in response to salt stress and gibberellic acid treatment. Research Journal of Agricultural and Biological Sciences, 3, 200–213.

  • Hasanuzzaman M, Nahar K, Fujita M (2013): Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In: Ahmad P, Azooz MM, Prasad MNV (eds): Ecophysiology and responses of plants under salt stress. Springer-Verlag, New York, 25–87.

  • Heath RL, Packer L (1968): Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125, 189–198.

  • Jaleel CA, Manivannan P, Murali PV, Gomathinayagam M, Panneerselvam R (2008): Antioxidant potential and indole alkaloid profile variations with water deficits along different parts of two varieties of Catharanthus roseus. Colloids and Surfaces B: Biointerfaces, 62, 312–318.

  • Jampeetong A, Brix H (2009): Effects of NaCl salinity on growth, morphology, photosynthesis and proline accumulation of Salvinia natans. Aquatic Botany, 91, 181–186.

  • Kaloyereas SA (1958): A new method for determining drought resistance. Plant Physiology, 33, 232–233.

  • Kar M, Feierabend J (1984): Metabolism of activated oxygen in detached wheat and rye leaves and its relevance to the initiation of senescence. Planta, 160, 385–939.

  • Kausar F, Shahbaz M (2013): Interactive effect of foliar application of nitric oxide (NO) and salinity on wheat (Triticum aestivum L.). Pakistan Journal of Botany, 45, 7–73.

  • Lokhande VH, Srivastava S, Patade VY, Dwivedi S, Tripathi RD, Nikam TD, Suprasanna P (2011): Investigation of arsenic accumulation and tolerance potential of Sesuvium portulacastrum (L.) L. Chemosphere, 82, 529–534.

  • Misra N, Saxena P (2009): Effect of salicylic acid on proline metabolism in lentil grown under salinity stress. Plant Science, 177, 181–189.

  • Murashige T, Skoog F (1962): A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum, 15, 473–497.

  • Qasim M, Ashraf M, Ashraf Y, Ahmad R, Nazli S (2004): Some growth related characteristics in canola (Brassica napus L.) under salinity stress. International Journal of Agriculture and Biology, 4, 665–668.

  • Rafiq M, Mali M, Khatri A, Dahot MU (2008): Callus induction and regeneration in local mungbean (Vigna radiate L. Wilczek) under salt stress. Journal of Biotechnology, 136, 147–169.

  • Sabir F, Sangwan RS, Kumar R, Neelam S (2012): Salt stress-induced responses in growth and metabolism in callus cultures and differentiating in vitro shoots of Indian ginseng (Withania somnifera Dunal). Journal of Plant Growth Regulation, 31, 537–548.

  • Shahbaz M, Zia B (2011): Does exogenous application of glycine betaine through rooting medium alter rice (Oryza sativa L.) mineral nutrient status under saline conditions? Journal of Applied Botany and Food Quality, 84, 54–60.

  • Shaheen S, Naseer S, Ashraf M, Akram N (2012): Salt stress affects water relations, photosynthesis and oxidative defense mechanisms in Solanum melongena L. Journal of Plant Interaction, 8, 85–96.

  • Sharma P, Sardana V, Banga SS (2013): Salt tolerance of Indian mustard (Brassica juncea) at germination and early seedling growth. Environmental and Experimental Biology, 11, 39–46.

  • Siler B, Misic D, Filipovic B, Popovic Z, Cvetic T, Mijovic A (2007): Effects of salinity on in vitro growth and photosynthesis of common centaury (Centaurium erythraea Rafn.). Archives of Biological Sciences, 59, 129–134.

  • Simaei M, Khavarinejad A, Saadatmand S, Bernard F, Fahimi H (2011): Interactive effects of salycylic acid and nitric oxide on soybean plants under NaCl salinity. Russian Journal of Plant Physiology, 58, 783–790.

  • Taffouo VD, Wamba OF, Yombi E, Nono GV, Akoe A (2010): Growth, yield, water status and ionic distribution response of three bambara groundnut (Vigna subterranean (L.) Verdc.) landraces grown under saline conditions. International Journal of Botany, 6, 53–58.

  • Tort N, Turkyilmaz B (2004): A physiological investigation on the mechanisms of salinity tolerance in some barley culture forms. Journal of Forest Science, 27, 1–16.

  • Turan MA, Kalkat V, Taban S (2007): Salinity-induced stomatal resistance, proline, chlorophyll and ion concentrations of bean. International Journal of Agricultural Research, 2, 483–488.

  • Turkan I, Demiral T (2009): Recent developments in understanding salinity tolerance. Environmental and Experimental Botany, 67, 2–9.

  • Uhvits R (1964): Effects of osmotic pressure on water absorption and germination of alfalfa seeds. American Journal of Botany, 33, 278–285.

  • Zhu JK (2002): Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 53, 247–273.

  • Zubr J (1997): Oil-seed crop: Camelina sativa. Industrial Crop Production, 6, 113–119.

Scientia Agriculturae Bohemica

The Journal of Czech University of Life Sciences Prague

Journal Information

CiteScore 2016: 0.78

SCImago Journal Rank (SJR) 2016: 0.398
Source Normalized Impact per Paper (SNIP) 2016: 0.688


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