Assessment of Serum Ischemia-modified albumin, Prolidase and Thiol-Disulphide Levels in Subjects With Breast Cancer

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Background: Oxidative damage is of great importance for patients with breast cancer. Thus, studies were performed to identify the relationship between breast cancer and oxidative stress biomarkers.

Objectives: In this study, our aim was to find out the oxidative and antioxidant status, serum thiol-disulphide levels in subjects with breast cancer.

Methods: This study was conducted between March and June 2018 with 82 control subjects (aged between 32-67 years) and 127 breast cancer patients (aged between 27-66 years) (p=0.058) in Selcuk University Faculty of Medicine, Konya, Turkey. Serum myeloperoxidase (MPO), catalase, prolidase were analyzed with kinetic spectrophotometric and thiol-disulphide, ischemia-modified albumin (IMA), ceruloplasmin were detected by colorimetric methods.

Results: Serum levels of catalase [199.3 (16.4-489.9) vs 81.6 (18.2-322.9) (kU/L)], MPO [124±28 vs 101±31 U/L], disulphide [25 (11-61) vs 18 (2-41) µmol/L], IMA [0.66 (0.31-3.30) vs 0.62 (0.19-1.31) absorbance unit (ABSU)] and prolidase levels [2217±538 vs 1456±401 U/L] were higher in patients than control subjects (For all p<0.001 except for IMA p=0.031). Native thiol [342±60 vs 391±52 µmol/L] and total thiol levels [396±56 vs 430±52 µmol/L] were lower in patients compared with the control group (For all p<0.001).

Conclusions: Levels of serum thiol/disulphide and prolidase might be reliable indicators for determining oxidative status in certain patient populations.

1. Hecht F, Pessoa CF, Gentile LB, Rosenthal D, Carvalho DP, Fortunato RS. The role of oxidative stress on breast cancer development and therapy. Tumour Biol. 2016 Apr; 37(4):4281-91. DOI: 10.1007/s13277-016-4873-9

2. Mencalha A, Victorino VJ, Cecchini R, Panis C. Mapping oxidative changes in breast cancer:understanding the basic to reach the clinics. Anticancer Res. 2014 Mar; 34(3):1127-40.

3. Guney T, Kanat IF, Alkan A, Alisik M, Akinci S, Silay K, et al. Assessment of serum thiol/disulfide homeostasis in multiple myeloma patients by a new method. Redox Rep. 2017 Nov; 22(6):246-51. DOI: 10.1080/13510002.2016.1180100

4. Elmas B, Erel O, Ersavaş D, Yurumez Y. Thiol/disulfide homeostasis as a novel indicator of oxidative stress in children with simple febrile seizures. Neurol Sci. 2017 Nov; 38(11):1969-75. DOI: 10.1007/s10072-017-3087-2

5. Coelho BA, Belo AV, Andrade SP, Amorim WC, Uemura G, da Silva Filho AL. N-acetylglucosaminidase, myeloperoxidase and vascular endothelial growth factor serum levels in breast cancer patients. Biomed Pharmacother. 2014 Mar; 68(2):185-9. DOI: 10.1016/j.biopha.2013.10.009

6. Jena I, Nayak SR, Behera S, Singh B, Ray S, Jena D, et al. Evaluation of ischemia-modified albumin, oxidative stress, and antioxidant status in acute ischemic stroke patients. J Nat Sci Biol Med. 2017 Jan-Jun; 8(1):110-113. DOI: 10.4103/0976-9668.198346

7. Kucukdurmaz F, Efe E, Celik A, Dagli H, Kılınc M, Resim S. Evaluation of serum prolidase activity and oxidative stress markers in men with BPH and prostate cancer. BMC Urol. 2017 Dec; 17(1):116. DOI: 10.1186/s12894-017-0303-6

8. Erel O. Neselioglu S. A novel and automated assay for thiol/disulfide homeostasis. Clin Biochem. 2014 Dec; 47(18):326-32. DOI: 10.1016/j.clinbiochem.2014.09.026

9. Goth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta. 1991 Feb; 196(2-3):143-152. DOI: 10.1016/0009-8981(91)90067-M

10. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation:estimation of neutrophil content with an enzyme marker. J Invest Dermatol. 1982 Mar; 78(3):206–9. DOI: 10.1111/1523-1747.ep12506462

11. Erel O. Automated measurement of serum ferroxidase activity. Clin Chem. 1998 Nov; 44(11):313–9.

12. Myara I, Myara A, Mangeot M, Fabre M, Charpentier C, Lemonnier A. Plasma prolidase activity:a possible index of collagen catabolism in chronic liver disease. Clin Chem. 1984 Feb; 30(2):211-5.

13. Myara I, Charpentier C, Lemonnier A. Optimal conditions for prolidase assay by proline colorimetric determination:application to iminodipeptiduria. Clin Chim Acta. 1982 Oct; 125(2):193-205. DOI: 10.1016/0009-8981(82)90196-6

14. Chinard FP. Photometric estimation of proline and ornithine. J Biol Chem. 1952 Nov; 199(1):91-95.

15. Bar-Or D, Lau E, Winkler JV. A novel assay for cobalt-albumin binding and its potential as a marker for myocardial ischemia-a preliminary report. J Emerg Med. 2000 Nov; 19(4):311-5. DOI: 10.1016/S0736-4679(00)00255-9

16. Badid N, Ahmed FZ, Merzouk H, Belbraouet S, Mokhtari N, Merzouk SA, et al.. Oxidant/antioxidant status, lipids and hormonal profile in overweight women with breast cancer. Pathol Oncol Res. 2010 Jun; 16(2):159-67. DOI: 10.1007/s12253-009-9199-0

17. Punnonen K, Ahotupa M, Asaishi K, Hyöty M, Kudo R, Punnonen R. Antioxidant enzyme activities and oxidative stress in human breast cancer. J Cancer Res Clin Oncol. 1994 Jun; 120(6):374-7. DOI: 10.1007/BF01247464

18. Ramírez-Expósito MJ, Urbano-Polo N, Due-as B, Navarro-Cecilia J, Ramírez-Tortosa C, Martín-Salvago MD, et al. Redox status in the sentinel lymph node of women with breast cancer. Ups J Med Sci. 2017 Dec; 122(4):207-16. DOI: 10.1080/03009734.2017.1403522

19. Coelho BA, Belo AV, Andrade SP, Amorim WC, Uemura G, da Silva Filho AL. N-acetylglucosaminidase, myeloperoxidase and vascular endothelial growth factor serum levelsin breast cancer patients. Biomed Pharmacother. 2014 Mar; 68(2):185-9. DOI: 10.1016/j.biopha.2013.10.009

20. Topuz M, Şen O, Kaplan M, Akkus O, Erel O, Gur M. The Role of Thiol/Disulphide Homeostasis in Anthracycline Associated Cardiac Toxicity. Int Heart J. 2017 Feb; 58(1):69-72. DOI: 10.1536/ihj.16-124

21. Omma A, Sandikci SC, Kücüksahin O, Alisik M, Erel O. Can the Thiol / Disulfide Imbalance Be a Predictor of Colchicine Resistance in Familial Mediterranean Fever? J Korean Med Sci. 2017 Oct; 32(10):1588-94. DOI: 10.3346/jkms.2017.32.10.1588

22. Fidan F, Alkan BM, Ugurlu FG, Bozkurt S, Sezer N, Bicer C, et al. Dynamic Thiol/Disulphide Homeostasis in Patients With Fibromyalgia. Arch Rheumatol. 2017 Jul; 32(2):112-7. DOI: 10.5606/ArchRheumatol.2017.5931

23. Altıparmak IH, Erkuş ME, Sezen H, Demirbag R, Gunebakmaz O, Kaya Z, et al. The relation of serum thiol levels and thiol/disulphide homeostasis with the severity of coronary artery disease. Kardiol Pol. 2016 May; 74(11):1346-53. DOI: 10.5603/KP.a2016.0085

24. Dirican N, Dirican A, Sen O, Aynali A, Atalay S, Bircan HA, et al. Thiol/disulfide homeostasis:A prognostic biomarker for patients with advanced non-small cell-lung cancer? Redox Rep. 2016 Sep; 21(5):197-203. DOI: 10.1179/1351000215Y.0000000027

25. Luan XD, Zhao KH, Hou H, Gai YH, Wang QT, Mu Q, et al. Changes in ischemia modified albumin in myocardial toxicity induced by anthracycline and docetaxel chemotherapy. Medicine (Baltimore). 2017 Aug; 96(32):e7681. DOI: 10.1097/MD.0000000000007681

26. Ma Y, Kang W, Bao Y, Jiao F, Ma Y. Clinical significance of ischemia-modified albumin in the diagnosis of doxorubicin-inducedmyocardial injury in breast cancer patients. PLoS One. 2013 Nov; 8(11):e79426. DOI: 10.1371/journal.pone.0079426

Revista Romana de Medicina de Laborator

Romanian Journal of Laboratory Medicine

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IMPACT FACTOR 2018: 0,800
5-year IMPACT FACTOR: 0,655

CiteScore 2017: 0.31

SCImago Journal Rank (SJR) 2018: 0.194
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