The dynamics of some oxidative stress markers in 3, 6 and 12-months alcohol abstinent patients: possible relevance for the usage of antioxidants in alcohol withdrawal / Dinamica unor markeri ai stresului oxidativ la 3, 6 şi 12 luni de abstinenţă de la alcool: posibila relevanţă a utilizării de antioxidanţi

Open access

Abstract

While the exact relevance of the oxidative stress markers after the complex processes of alcohol withdrawal is still controversial, in the present report we were interested in studying the relevance of oxidative stress status in the alcohol withdrawal processes, by determining some oxidative stress markers after 3, 6 and 12 months of abstinence. 62 patients were selected, all of them males. Thus, 33 (baseline), 14 (3 months), 14 (6 months) and 15 (12 months) patients, while the control group (n=32) included healthy, sex and aged-matched subjects. Regarding superoxid dismutase, we observed a significant group difference (p<0.0001), together with an increase in all 3 cases of time-abstinence, as compared to baseline results: (p<0.0001-3 months), (p<0.0001-6 months) and (p<0.0001- 12 months). Also for glutathione peroxidase, we observed a significant overall effect of the abstinence in our groups (p=0.0003), plus an increase especially at 6 months (p=0.03) and 12 months (p=0.006). Regarding malondialdehyde, as a main marker for the lipid peroxidation processes, we found significant differences between our groups (p<0.0001), together with a decrease in all 3 cases, compared to the baseline group (p=0.003), (p=0.01) and (p=0.0002). In conclusion, this confirms the increased oxidative stress status in alcoholic patients and even more importantly, we showed that there is a significant and progressive decrease in the oxidative stress status at 3, 6 and 12 months after the withdrawal process, as demonstrated by the increased levels of antioxidant enzymes and decreased rate of lipid peroxidation, when compared to baseline values.

1. Zima T, Fialová L, Mestek O, Janebová M, Crkovská J, Malbohan I, et al. Oxidative stress, metabolism of ethanol and alcohol-related diseases. J Biomed Sci. 2001 Jan-Feb;8(1):59-70. DOI: 10.1007/BF02255972

2. Guemouri L, Lecomte E, Herbeth B. Blood activities of antioxidant enzymes in alcoholics before and after withdrawal. J Stud Alcohol. 1993 Sep;54(5):626-9.

3. Huang MC, Chen CH, Peng FC, Tang SH, Chen C. Alterations in oxidative stress status during early alcohol withdrawal in alcoholic patients. J Formos Med Assoc. 2009 Jul;108(7):560-9. DOI: 10.1016/S0929-6646(09)60374-0

4. Lecomte E, Herberlk B, Pirollet P, Chanterelle Y. Effect of alcohol consumption on blood antioxidant nutrients and oxidative stress indicators. Am J Clin Nutr. 1994 Aug;60(2):255-261.

5. Girre C, Hispard E, Therond P. Effect of abstinence from alcohol on the depression of glutathione peroxidase activity and selenium and vitamin E levels in chronic alcoholic patients. Alcohol Clin Exp Res. 1990 Dec;14(6):909-12. DOI: 10.1111/j.1530-0277.1990. tb01836.x

6. Soardo G, Donnini D, Varutti R. Alcohol-induced endothelial changes are associated with oxidative stress and are rapidly reversed after withdrawal. Alcohol Clin Exp Res. 2005 Oct;29(10):1889-98. DOI: 10.1097/01. alc.0000183004.28587.23

7. Peng FC, Tang SH, Huang MC, Chen CC, Kuo TL, Yin S. Oxidative status in patients with alcohol dependence: a clinical study in Taiwan. J Toxicol Environ Health A. 2005 Sep;68(17-18);1497-509. DOI: 10.1080/15287390590967432

8. Situnayake RD, Crump BJ, Thurnham D. Lipid peroxidation and hepatic antioxidants in alcoholic liver disease. Gut 1990 Nov;31(11):1311-7. DOI: 10.1136/ gut.31.11.1311

9. Alexinschi O, Chirita R., Ciobica A., Padurariu M., Dobrin R., Prepelita R. et al. The relevance of oxidative stress status in one week and one month alcohol abstinent patients. J Med Biochem. 2014, DOI: 10.2478/ jomb-2014-0008. DOI: 10.2478/jomb-2014-0008

10. Schmedes A, Hølmer G. A new thiobarbituric acid (TBA) method for determining free malondialdehyde (MDA) and hydroperoxides selectively as a measure of lipid peroxidation. Journal of the American Oil Chemists Society. 1989 June; 66(6):813-817. DOI: 10.1007/ BF02653674

11. Sinha-Hikim I, Shen R, Paul W, Crum A, Vaziri N, Norris K. Effects of a novel cystine-based glutathione precursor on oxidative stress in vascular smooth muscle cells. Am J Physiol Cell Physiol. 2010 Sep;299(3):638-42 DOI: 10.1152/ajpcell.00434.2009

12. Otis JS, Guidot D. Procysteine increases alcohol-depleted glutathione stores in rat plantaris following a period of abstinence. Alcohol. 2010 Nov-Dec;45(6):495-500. DOI: 10.1093/alcalc/agq066

13. Seiva FR, Amauchi JF, Rocha KK, Ebaid GX, Souza G, Fernandes AA et al. Alcoholism and alcohol abstinence: N-acetylcysteine to improve energy expenditure, myocardial oxidative stress, and energy metabolism in alcoholic heart disease. Alcohol. 2009 Dec;43(8):649-56. DOI: 10.1016/j.alcohol.2009.09.028

14. Ozaras R, Tahan V, Aydin S, Uzun H, Kaya S, Sen turk H. N-acetylcysteine attenuates alcohol-induced oxidative stress in rats. World J Gastroenterol. 2003 Apr;9(4):791-794.

15. Seiva FR, Amauchi JF, Rocha KK, Ebaid GX, Souza G, Fernandes AA, et al. Alcoholism and alcohol abstinence: N-acetylcysteine to improve energy expenditure, myocardial oxidative stress, and energy metabolism in alcoholic heart disease. Alcohol. 2009 Dec;43(8):649-56. DOI: 10.1016/j.alcohol.2009.09.028

16. Diniz YS, Rocha K, Souza G, Galhardi C, Ebaid G, Novelli Filho JL, et al. Effects of N-acetylcysteine on sucrose-rich diet-induced hyperglycaemia, dyslipidemia and oxidative stress in rats. Eur J Pharmacol. 2006 Aug;543(1-3):151-157. DOI: 10.1016/j.ejphar. 2006.05.039

17. Sies H. Oxidative stress: oxidants and antioxidants. Experimental Physiology. 1997 Mar;82(2):291-295.

Revista Romana de Medicina de Laborator

Romanian Journal of Laboratory Medicine

Journal Information


IMPACT FACTOR 2018: 0,800
5-year IMPACT FACTOR: 0,655



CiteScore 2017: 0.31

SCImago Journal Rank (SJR) 2018: 0.194
Source Normalized Impact per Paper (SNIP) 2018: 0.306

Cited By

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 233 207 19
PDF Downloads 102 96 7