Detection and serotyping of Listeria monocytogenes in some food products from North-East of Romania

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Abstract

Listeria monocytogenes represents an important hazard to human health because it is capable of causing listeriosis, an atypical foodborne disease with a high fatality rate.The aim of this study was to detect and serotype Listeria monocytogenes in some food products (of animal and vegetable origin), collected during the 2011 period. The detection of Listeria monocytogenes was performed by ISO 11290- 1-A1/2004 standard method and verified by real-time PCR. Numerous subtyping strategies have been developed to characterize Listeria monocytogenes isolates. Serotyping, the traditional method for the subtype characterization of Listeria monocytogenes is not very discriminatory, but it is a universal method which gives important information on prevalence of specific serotypes. The confirmation of Listeria monocytogenes isolated strains was performed by a TaqMan real-time PCR assay, using TaqMan Pathogen Detection Kits (Applied Biosystems). The assay use specific primers and a probe for Listeria monocytogenes. In this study on a total number of 260 samples, 25 (9.61%) gave positive results for Listeria monocytogenes by conventional method and only 22 (8.46%) of them were confirmed by real-time PCR. These results indicate a high efficiency of the real-time PCR method in a short time, compared with classical methods which can provide false positive results. Four different Listeria monocytogenes serotypes were found in nine varieties of food products: 1/2a, 1/2b, 1/2c and 4b

1. Duodu S, Mehmeti I, Holst-Jensen A, Loncarevic S. Improved sample preparation for Real-Time PCR detection of Listeria monocytogenes in hot-smoked salmon using filtering and immunomagnetic separation techniques. Food Anal Method, 2009; 2:23-9.

2. Vanegas MC, Vasquez E, Martinez AJ, Rueda A. Detection of Listeria monocytogenes in raw whole milk for human consumption in Colombia by real-time PCR. Food Cont. 2009; 20:430-432.

3. Jemmi T, Stephen R. Listeria monocytogenes: foodborne pathogen and hygiene indicator. Rev Sci Tech OIE, 2006; 25:571-80.

4. Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees): Chapter 2.9.7. Listeria monocytogenes, Vol. II, Sixth Edition, OIE Biological Standards Commision, 2008; 1238-1254

5. Olsen JE. DNA-based methods for detection of foodborne bacterial pathogens. Food Res Int, 2000; 33:257-66.

6. Carp-Cărare C. Bacteriological researches on Listeria monocytogenes and its epidemiological implications. Doctoral thesis, USAMV Iasi, 2006.

7. http://www.eurosurveillance.org/viewarticle.aspx?articleid=19832

8. Janzten M, Navas J, Corujo A, Moreno R, López V, Martínez-Suárez JV. Review. Specific detection of Listeria monocytogenes in foods using commercial methods: from chromogenic media to real-time PCR. Span J Agric Res, 2006; 4:235-47.

9. Scotter SL, Langton S, Lombard B, Sculten S, Nagelkerke N, In’t Veld PH, et al Validation of ISO method 11290 part 1- detection of Listeria monocytogenes in foods. Int J Food Microbiol, 2001; 64(3):295-306.

10. Rodriguez-Lazaro D, Jofre A, Aymerich T, Hugas M, Pla M. Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR. Appl Environ Microbiol, 2004; 70:6299-301.

11. Oliveira MMM, Brugnera DF, Alves E, Piccoli RH. Biofilm formation by Listeria monocytogenes on stainless steel surface and biotransfer potential. Braz J Microbiol, 2010; 41:97-106.

12. Barocci S, Calza L, Blasi G, Briscolini S, De Curtis M, Palombo B, et al. Evaluation of a rapid molecular method for detection of Listeria monocytogenes directly from enrichment broth media. Food control, 2008; 19:750-6.

13. O'Grady J, Ruttledge M, Sedano-Balbas S, Smithy TJ, Barry T, Maher M. Rapid detection of Listeria monocytogenes in food using culture enrichment combined with real-time PCR. Food Microbiol, 2009; 26:4-7.

14. O'Grady J, Sedano-Balbas S, Maher M, Smith T, Barry T. Rapid real-time PCR detection of Listeria monocytogenes in enriched food samples based on the ssrA gene, a novel diagnostic target. Food Microbiol, 2008; 25:75-84.

15. Carp-Cărare C, Vlad-Sabie A, Horhogea C, Rîmbu C. Research regarding detection of Listeria monocytogenes from animal food products, Rev Rom Med Vet, 2011; 21 (2):187-194

16. Kirkan Ş, Göksoy EÖ, Kaya O. Detection of Listeria monocytogenes by using PCR in Helix pomatia. Turk J Vet Anim Sci, 2006; 30:375-80.

17. Berrada H, Soriano J, Pico Y, Manes J. Quantification of Listeria monocytogenes in salads by real time quantitative PCR. Intl J Food Microbiol, 2006; 107:202-6.

18. vonLaer AE, Lima AS, Trindade PS, Andriguetto C, Destro M, Silva WP. Characterization of Listeria monocytogenes isolated from a fresh mixed sausage processing line in Pelotas-RS by PFGE. Braz J Microbiol, 2009; 40:574-82.

19. Mullapudi S, Siletzky RM, Kathariou S. Heavy metal and benzalkonium cloride resistance of L. monocytogenes isolates from enrichment of Turkey processing plants. Appl Environ Microbiol, 2008; 74(5):1464-1468.

20. Lundén J, Tolvanen R, Korkeala H. Human listeriosis outbreaks linked to dairy products in Europe. J Dairy Sci, 2004; 87:E6-E12.

21. Gasanov U, Hughes D, Hansbro PM. Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review. FEMS Microbiol Rev, 2005; 29:851-75.

Revista Romana de Medicina de Laborator

Romanian Journal of Laboratory Medicine

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