Correlations Between the Autolytic Changes and Postmortem Interval in Refrigerated Cadavers

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Introduction. In forensic pathology the autolytic process has been observed and documented in order to determine the postmortem interval as accurately as possible. The observation and experiments have been carried out on cadavers exposed to environmental conditions – heat, humidity, air currents, soil, water.

Methods. For this study hematoxylin and eosin (HE) stained sections of organ samples from 30 autopsied bodies were examined under the microscope. Modifications of tissue and cell structures were noted in correlation with the bodies’ time spent in the Morgue’s mortuary refrigerator until the autopsy was performed, which varied between 24 hours and 22 days.

Results. All the organs sampled (lung, heart, liver and pancreas) showed severe autolytic alterations after 5 to 8 days. The most heavily affected was the pancreas, cells within Langherhans islets becoming complete autolyzed at the 36 hours mark. Inside organs, autolytic processes occur at different rates depending on the locations within that organ –deeply or superficial; in the heart after 4 or more days subendocardic myocardium shows less severe autolytic changes than the subepicardial one.

Conclusion. Autolytic processes have a delayed onset and a much lower progression rate in a cold controlled environment. Different organs suffer different rates of autolysis in correlation to their structure and enzymatic content.


  • 1. CELATA EN. Postmortem Intervals in Mice Submerged in Aqueous Environments at 20°C. J Forensic Sci. 2015 Aug 12. doi: 10.1111/1556-4029.12850.

  • 2. COCKLE DL, BELL LS. Human decomposition and the reliability of a 'Universal' model for post mortem interval estimations. Forensic Sci Int 2015; 253:136.e1-9.

  • 3. MATUSZEWSKI S, KONWERSKI S, FRĄTCZAK K, SZAFAŁOWICZ M. Effect of body mass and clothing on decomposition of pig carcasses. Int J Legal Med. 2014; 128(6):1039-48.

  • 4. DERMENGIU D. Patologie medico-legală. Editura Viaţa medicală românească, 2002.

  • 5. ZDRAVKOVIĆ M, KOSTOV M, STOJANOVIĆ M. Identification of postmortem autolytic changes on the kidney tissue using PAS stained method. Facta Universitatis, 2006; 13(3):181-184.

  • 6. NERY LR, MOREIRA CR, CESTARI TM, TAGA R, DAMANTE JH. Postmortem acinar autolysis in rat sublingual gland: a morphometric study. J Appl Oral Sci. 2010; 18(5):509-14.

  • 7. LEGEA 104/2003 privind manipularea cadavrelor umane şi prelevarea organelor şi ţesuturilor de la cadavre în vederea transplantului republicată 2014. Legea nr. 104/2003 republicată 2014, Monitorul Oficial, Partea I, nr. 213 din 25 martie 2014.

  • 8. HOTĂRÂRE nr. 451 din 1 aprilie 2004 pentru aprobarea Normelor metodologice de aplicare a Legii nr. 104/2003 privind manipularea cadavrelor umane şi prelevarea organelor şi ţesuturilor de la cadavre în vederea transplantului, Monitorul Oficial nr., 340/19 apr. 2004.

  • 9. DIMAIO VJ, DIMAIO D. Forensic Pathology, Second Edition, CRC Press LLC, 2001.

  • 10. RUTTY GN. Essentials of autopsy practice current methods and modern trends, Springer-Verlag London Limited, 2006.

  • 11. MAJNO G, JORIS I. Cells, Tissues and Disease: Principles of General Pathology, 2nd Edition, Oxford University Press, 2004.

  • 12. KARADŽIĆ R, ILIĆ G, ANTOVIĆ A, BANOVIĆ LK. Autolytic ultrastructural changes in rat and human hepatocytes. Rom J Leg Med 2010; 18:247 – 52.

  • 13. GUNDLACH G, LUTTERMANN-SEMMER E. The effect of pH and temperature on the stability and enzymatic activity of prostatic acid phosphatase. Studies on the optimization of a continuous monitored determination of acid phosphatase. J Clin Chem Clin Biochem. 1987; 25(7):441-6.

  • 14. JAY DIX, MICHAEL GRAHAM. Time of death, decomposition and identification: An atlas. CRC Press LLC, 2000.

  • 15. ANA LÍA HENRÍQUEZ HERRERA. Cambios cadavéricos e inmunohistoquímica para estimación de intervalo post mórtemenratas de laboratorio. Universidad de Chile, Santiago, Chile, 2013.

  • 16. SAUKKO P, KNIGHT B, KNIGHT’S Forensic Pathology. Third Edition, Edward Arnold (Publishers) Ltd, 2004.

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