Density of tryptase-positive mast cells correlated with the presence of H. pylori in gastric neoplasia

Open access


Background. Gastric cancer continues to be a platoon leader of mortality causes. A significant number of recent studies show direct or indirect involvement of mast cells (MC), with a complex role both pro- and anti-tumor growth.

Aim. To objectify the correlations between expression of MC and presence of Helicobacter pylori (HP) infection depending on neoplastic nature of the gastric damage.

Subjects and Methods. The study was carried out on archival samples of gastric wall from 30 patients with gastric cancer versus 30 age and sex-matched subjects with gastric surgery for nonneoplastic diseases. The inclusion criteria for the case group were histologically proven stage T3/T4 malignancies with regional lymph node metastases. For each case of the study group, distribution and number of MC tryptase positive (DMC–TP) were analyzed in five different areas from the same gastrectomy specimen: intratumor area, deep and side tumor invasion front, normal gastric tissue sample 5-10 cm or more distant from the tumor and furthest resection margin.

Results. Independently of HP infection, the study recorded a significantly lower value of DMC-TP in male patients. In regions with inflammatory lesions and preneoplastic changes and in control cases with non-gastric neoplasia, the DMC-TP level was higher than controls with HP-related inflammatory pathology, thus removing bacterial etiology from the forefront of MC mobilizing causes.

Conclusion. The presence of H. pylori infection was not found to cause significant changes in terms of mobilizing mast cells in the gastric wall with advanced tumors, with minimal stage III TNM.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. FOX JG WANG TC. Inflammation atrophy and gastric cancer. J Clin Invest 2007 117: 60-9.

  • 2. HOUGHTON J WANG T. Helicobacter pylori and gastric cancer: a new paradigm for inflammation-associated epithelial cancers. Gastroenterology 2005 128: 1567-78.

  • 3. NAKAJIMA S BAMBA N HATTORI T. Histological aspects and role of mast cells in Helicobacter pylori-infected gastritis. Alimentary Pharmacology and Therapeutics Supplement. 2004; 20(1): 165-70.

  • 4. MUKHERJEE S BANDYOPADHYAY G DUTTA C BHATTACHARYA A KARMAKAR R BARUI G. Evaluation of endoscopic biopsy in gastric lesions with a special reference to the significance of mast cell density. Indian Journal of Pathology and Microbiology. 2009; 52(1): 20-4.

  • 5. DALTON DK RANDOLPH JN. The roles of mast cells in anticancer immunity. Cancer Immunology Immunotherapy 2012; 61(9): 1511-20.

  • 6. MA Y HWANG RF LOGSDON CD ULLRICH SE. Dynamic Mast Cell–Stromal Cell Interactions Promote Growth of Pancreatic Cancer. Cancer Research 2013 73: 3927.

  • 7. WHITESIDE TL. The tumor microenvironment and its role in promoting tumor growth. Oncogene 2008 27: 5904-12.

  • 8. SAUTÈS-FRIDMAN C CHERFILS-VICINI J DAMOTTE D FISSON S FRIDMAN WH HERVÉ W CREMER I DIEUNOSJEAN MC. Tumor microenvironment is multifaceted. Cancer and Metastasis Reviews 2011 30(1):13-25.

  • 9. RIBATTI D VACCA A NICO B CRIVELLATO E RONCALI L DAMMACCO F. The role of mast cells in tumour angiogenesis. British Journal of Haematology 2001 115: 514-521.

  • 10. MANGIA A MALFETTONE A ROSSI R PARADISO A RANIERI G SIMONE G RESTA L. Tissue remodelling in breast cancer: human mast cell tryptase as an initiator of myofibroblast differentiation. Histopathology 2011 58:1096-1106.

  • 11. THEOHARIDES TC ALYSANDRATOS K ANGELIDOU A DELIVANIS D SISMANOPOULOS N ZHANG B et al. Mast cells and inflammation. Biochimica et Biophysica Acta - Molecular Basis of Disease. 2012; 1822(1):21-33.

  • 12. SIEMANN DW. Tumor Microenvironment. Publisher John Wiley & Sons 2010 pp.8-10.

  • 13. NORRBY K. Mast cells and angiogenesis. APMIS 2002 110:355-371.

  • 14. WAN WH FORTUNA MB FURMANSKI P. A rapid and efficient method for testing immunohistochemical reactivity of monoclonal antibodies against multiple tissue samples simultaneously. J. Immunol Methods. 1987 103(1):121-9.

  • 15. TAWEEVISIT M KLAIKAEW N. Correlation between mast cell density and histological parameters in Helicobacter pylori-associated gastritis. Asian Biomedicine. 2011; 5(1):163-7.

  • 16. YOSHII M JIKUHARA A MORI S IWAGAKI H TAKAHASHI HK NISHIBORI M et al. Mast cell tryptase stimulates DLD-1 carcinoma through prostaglandin-and MAP kinase-dependent manners. Journal of Pharmacological Sciences. 2005; 98(4):450-8.

  • 17. HODGES K KENNEDY L MENG F ALPINI G FRANCIS H. Mast cells disease and gastrointestinal cancer: A comprehensive review of recent findings. Transl. Gastrointest. Cancer. 2012 1(2): 138-150.

  • 18. KHAZAIE K BLATNER S KHAN MW GOUNARI F GOUNARIS E DENNIS K et al. The significant role of mast cells in cancer. Cancer Metastasis Rev. 2011 30(1): 45-60.

  • 19. QUESNEL B. Tumor dormancy and immunoescape. APMIS 2008 116(7-8): 685-694.

  • 20. NECHUSHTAN H. The complexity of the complicity of mast cells in cancer. Int J Biochem Cell Biol. 2010 42(5): 551-4.

  • 21. MARICHAL T TSAI M GALLI SJ. Mast cells: potential positive and negative roles in tumor biology. Cancer Immunol Res. 2013 1(5): 269-79.

  • 22. REBBER LL MARICHAL T GALLI SJ. New models for analyzing mast cell functions in vivo. Trends Immunol. 2012 33(12): 613-625.

  • 23. HÜTTER G GANEPOLAS S THIEL E HOFMANN WK. Inhibition of mast cell tryptase activity. A new therapeutic target against malignancy induced angiogenesis. Bioscience Hypotheses 2008 4(1): 213-217.

  • 24. KHATAMI M. Unresolved inflammation: ‘immune tsunami’ or erosion of integrity in immune-privileged and immune-responsive tissues and acute and chronic inflammatory diseases or cancer. Expert Opin Biol Ther. 2011 11(11): 1419-32.

  • 25. KHATAMI M. Chronic Inflammation: Synergistic Interactions of Recruiting Macrophages (TAMs) and Eosinophils (Eos) with Host Mast Cells (MCs) and Tumorigenesis in CALTs. M-CSF Suitable Biomarker for Cancer Diagnosis! Cancers (Basel) 2014 6(1): 297-322.

  • 26. WALKER ME HATFIELD JK BROWN MA. New insights into the role of mast cells in autoimmunity: evidence for a common mechanism of action? Biochim Biophys Acta. 2012 1822(1): 57-65.

  • 27. LIU J ZHANG Y ZHAO J YANG Z LI D KATIRAI F et al. Mast cell: insight into remodeling a tumor microenvironment. Cancer Metastasis Rev. 2011 30(2): 177-84.

  • 28. DA SILVA E JAMUR M OLIVER C. Mast Cell Function: A New Vision of an Old Cell. J Histochem Cytochem. 2014 62(10): 698-738.

  • 29. GALINSKY DS NECHUSHTAN H. Mast cells and cancer--no longer just basic science. Crit Rev Oncol Hematol. 2008 68(2): 115-30.

  • 30. AMMENDOLA M SACCO R DONATO G ZUCCALA V RUSSO E LUPOSELLA M et al. Mast cell positivity to tryptase correlates with metastatic lymph nodes in gastrointestinal cancer patients treated surgically. Oncology 2013 85(2): 111-6.

  • 31. PAYNE V KAM PC. Mast cell tryptase: a review of its physiology and clinical significance. Anaesthesia 2004 59(7): 695-703.

Journal information
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 232 117 5
PDF Downloads 104 64 2