Antidiabetic Activity of Different Extracts of Myrtus Communis in Streptozotocin Induced Diabetic Rats

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Background and aim: Hydroalcoholic (70°) extract of leaves of Myrtus communis has been shown to have antidiabetic effect in streptozotocin induced diabetic rats in our previous study. In this study, we intended to determine the components of the mentioned extract and identify the mechanism for its action.

Materials and Methods: The leaves of Myrtus communis were extracted using petroleum ether by soxhlet. 100 g of the powder remaining in the strainer soxhlet apparatus were placed in two different percolators. The extraction was carried out by percolation method with ethanol-water (1-1) or distilled water in 72 hours. The remaining powder of water extract was further extracted using again ethanol (percolation method). The study was conducted on forty-eight matured male Charles-River rats (200-300 g) divided into 6 groups (n=8). Diabetes mellitus was induced by single intraperitoneal injection of 35 mg/kg of streptozotocin (STZ). Hydroalcoholic, water, and ethanol extracts of Myrtus communis were used at the dose of 4, 2, and respectively 2 g/kg body weight per day for 5 days. All extracts were given orally by gastric tube.

Results: We found that the total hydroalcoholic extract of Myrtus communis leaves showed a moderate antidiabetic effect. In this study, we showed that the ethanolic extract of leaves (2 g/kg) had a better hypoglycemic effect in diabetic rats compared with the aqueous extract (p < 0.05). Our results also showed that the oral administration of the ethanolic extract (2 g/kg) had an additive effect on the hypoglycemic action of glibenclamide (oral administration 5 mg/kg) in rats.

Conclusion: A review of previous researches on leaves of Myrtus communis and also the present study suggests that the extracts may stimulate the β-cells of pancreas to release insulin.

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  • 1. Dabaghian FH Kamalinejad M Shojaei A Abdollahi Fard M. Presenting anti-diabetic plants in Iranian traditional medicine. J Diabetes Endocrinol 3: 70-76 2012.

  • 2. Pulipaka S Challa SR Pingili RB. Comparative antidiabetic activity of methanolic extract of Operculina turpethum stem and root against healthy and streptozotocin induced diabetic rats. Int Current Pharm J 1: 272-278 2012.

  • 3. International Diabetes Federation. IDF Diabetes Atlas 6th ed. Brussels: International Diabetes Federation 2013. Accessed at:

  • 4. Arumugam G Manjula P Paari N. A review: Anti diabetic medicinal plants used for diabetes mellitus. J Acute Disease 2: 196-200 2013.

  • 5. Shugang Li Shuxia Guo Fei He et al. Prevalence of diabetes mellitus and impaired fasting glucose associated with risk factors in rural Kazakh adults in Xinjiang China. Int J Environ Res Public Health 12: 554-565 2015.

  • 6. Kavipriya S Tamilselvan N Thirumalai T Arumugam A. Anti-diabetic effect of methanolic leaf extract of Pongamia pinnata on streptozotocin induced diabetic rats. J Coastal Life Med 1: 113-117 2013.

  • 7. Jansson SPO Fall K Brus O et al. Prevalence and incidence of diabetes mellitus: a nationwide population-based pharmaco-epidemiological study in Sweden. Diabet Med 32: 1319–1328 2015.

  • 8. Guariguata L Whiting DR Hambleton I et al. Global estimates of diabetes prevalence for 2013 and projections for 2035 for the IDF Diabetes Atlas. Diabetes Res Clin Pract 103: 137–149 2014.

  • 9. American Diabetes Association. Classification and diagnosis of diabetes. Sec 2. In: Standards of Medical Care in Diabetes - 2015. Diabetes Care 38 [Suppl. 1]: S8-S16 2015.

  • 10. Jarald E Joshi SB Chandra Jain D. Diabetes and herbal medicines. Iran J Pharm Therap 7: 97-106 2008.

  • 11. Ahangarpour A Teymuri Zamaneh H Jabari A Malekshahi Nia H Heidari H. Antidiabetic and hypolipidemic effects of Dorema aucheri hydroalcoholic leave extract in streptozotocin-nicotinamide induced type 2 diabetes in male rats. Iran J Basic Med Sci 17: 808-814 2014.

  • 12. Azadbakht M Safapour S Ahmadi A Ghasemi M Shokrzadeh M. Anti-diabetic effects of aqueous fruits extract of Diospyros lotus L. on streptozotocin-induced diabetic rats and the possible morphologic changes in the liver kidney and heart. J Pharmacog Phyt 2: 10-16 2010.

  • 13. Ayaz NO. Antidiabetic and renoprotective effects of water extract of Rosmarinus officinalis in streptozotocin-induced diabetic rat. Afr J Pharm Pharmacol 6: 2664-2669 2012.

  • 14. Marles RJ Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine 12: 137-165 1995.

  • 15. Jayasri MA Gunasekaran S Radha A Mathew TL. Anti-diabetic effect of Costus pictus leaves in normal and streptozotocin-induced diabetic rats. Int J Diabetes Metab 16: 117-122 2008.

  • 16. Alarcon-Aguilara FJ Roman-Ramos R Perez-Gutierrez S Aguilar-Contreras A Contreras-Weber CC Flores-Saen JL. Study of the anti-hyperglycemic effect of plants used as antidiabetics. J Ethnopharmcol 61: 101–110 1998.

  • 17. Aghilli Khorassani MH. Makhzan-ol-Advieh. Islamic revolution education publication 1992.

  • 18. Sumbul S Aftab Ahmad M Asif M Akhtar M. Myrtus communis Linn. A review. Ind J Nat Prod Resour 2: 395-402 2011.

  • 19. Hosseinzadeh H Khoshdel M Ghorbani M. Antinociceptive anti-inflammatory effects and acute toxicity of aqueous and ethanolic extracts of myrtus communis l. aerial parts in mice. J Acupunct Meridian Stud 4: 242-247 2011.

  • 20. Johari H Nozari M Moghtari M Zamani Z Yazdani M. The effect of myrtus communis extract on liver enzymes and blood biochemical factors in diabetic adult male rats. Zahedan J Res Med Sci 16: 12-17 2014.

  • 21. Ferchichi H Salouage I Bacha S et al. Effect of myrtus communis l. extracts on attenuation of liver normothermic ischemia-reperfusion injury. Int J Transplant Technol Res S3: 001 2011. doi:10.4172/2161-0991.S3-001.

  • 22. Malekpour A Dehghani S Zahedi S Eskandari F. Effects of the hydro-ethanol extract of myrtus communis l. on blood glucose level and histopathological changes in alloxan-induced diabetic rats. Middle-East J Scient Research 12: 517-522 2012.

  • 23. Jia Z Tang M Wu J. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64: 555-559 1999.

  • 24. Shams Ardekani MR Hajimahmoodi M Oveisi MR et al. Comparative antioxidant activity and total flavonoid content of Persian pomegranate (punicagranatum l.) cultivars. Iran J Pharm Res 10: 519-524 2011.

  • 25. Katbab F Panjeshahin MR Azadbakht M. Antidiabetic activity of hydroalcoholic extract of myrtus communis in streptozotocin-induced diabetic Rats. Pharm D. Thesis Faculty of Pharmacy Shiraz University of Medical Sciences Shiraz Iran 2000.

  • 26. Hussin Mohamed EA Yam MF Ang LF Mohamed AJ Asmawi MZ. Antidiabetic properties and mechanism of action of orthosiphon stamineus enth bioactive sub-fraction in streptozotocin-induced diabetic rats. J Acupunct Meridian Stud 6: 31-40 2013.

  • 27. Elfellah MS Akhter M.H Khan MT. Anti-hyperglycemic effect of an extract of Myrtus communis in streptozotocin-induced diabetes in mice. J Ethnopharmacol 11: 275-281 1984.

  • 28. Sundarrajan T Kumar TR Udhaya Kumar E Sekar M Senthil Kumar M. Antidiabetic activity of methanolic extract of Hibiscus cannabinnus in streptozotocin induced diabetic rats. Int J Pharm Bio Sciences 2: 125-130 2011.

  • 29. Kumar A Ilavarasan R Jayachandran T et al. Anti-diabetic activity of Syzygium cumini and its isolated compound against streptozotocin-induced diabetic rats. J Med Plants Res 2: 246-249 2008.

  • 30. Sumbul S Ahmad MA Asif M Akhtar M. Myrtus communis Linn. A review. Ind J Nat Prod Resour 2: 395-402 2011.

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