The Effect of Pumpkin on GLP-1 and HOMA-β in Hypercholesterolemic Rats

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Abstract

Background and aim: High fat and fructose diet may impair β cell function through oxidative stress that is induced by subsequent hypercholesterolemia. The β cell function is usually quantified by homeostatic model assessment beta-cell function (HOMA-β). Oxidative stress may be decreased by β-carotene from pumpkin (Cucurbita maxima). This study aimed to evaluate the effects of pumpkin powder on glucagon-like peptide-1 (GLP-1) level and HOMA-β in rats with high fat and fructose diet.

Material and method: A total 25 rats were administered a high fat and fructose diet during 25 days. They were divided into five groups 1) normal, 2) hypercholesterolemic rats 3) hypercholesterolemic rats with 0.16 g pumpkin/200g bodyweight (BW); 4) hypercholesterolemic rats with 0.32 g pumpkin/200 g BW, and 5) hypercholesterolemic rats with 0.64 g of pumpkin/200 g BW. The lipid levels were measured before and after administration of pumpkin for 4 weeks, and at the end of the study, GLP-1 level and HOMA-β were analyzed.

Results: Administration of pumpkin to the rats on a high fat and fructose diet reduced total cholesterol, triglyceride, and increased HDL levels. Changes were positively correlated with the amount of pumpkin intake. The decrease of cholesterol levels was positively associated with GLP-1 level, and negatively correlated with HOMA-β

Conclusions: This study suggested that pumpkin can improve the HOMA-β and decrease GLP-1 levels, possibly by reducing cholesterol levels.

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  • 1. Bardini G Rotella CM Giannini S. Dyslipidemia and diabetes: reciprocal impact of impaired lipid metabolism and beta-cell dysfunction on micro- and macrovascular complications. Rev Diabet Stud 9: 82-93 2012.

  • 2. Dhingra D Lamba D Kumar R Nath P Gauttam S. Antihyperlipidemic activity of Aloe succotrina in rats: possibly mediated by inhibition of HMG-CoA reductase. ISRN Pharmacology 2014: 243575 2014.

  • 3. Hao M Head WS Gunawardana SC Hasty AH Piston DW. Direct effect of cholesterol on insulin secretion: a novel mechanism for pancreatic β Cell dysfunction. Diabetes 56: 2328-2338 2007.

  • 4. Wadden D Cahill F Amini P et al. Circulating glucagon-like peptide-1 increases in response to short-term overfeeding in men. Nutr Metab (Lond) 10: 33 2013.

  • 5. Garber AJ. Incretin effects on β-Cell function replication and mass: the human perspective. Diabetes Care 34[Suppl 2]: S258-S263 2011.

  • 6. Gupta V. Glucagon-like peptide-1 analogues: An overview. Indian J Endocrinol Metab 17: 413-421 2013.

  • 7. Abuohashish HM Aleisa AM Ahmed MM Alkhamees OA Al-Rejaie SS Alroujayee AS. Ameliorative effects of rutin and ascorbic acid combination on hypercholesterolemia-induced hepatotoxicity in female rats. Afr J Pharm Pharmacol 7: 280–288 2013.

  • 8. Kaneto H Matsuoka T. Role of pancreatic transcription factors in maintenance of mature β-Cell function. Int J Mol Sci 16: 6281-6297 2015.

  • 9. Xu G Kaneto H Laybutt DR et al. Downregulation of GLP-1 and GIP receptor expression by hyperglycemia: possible contribution to impaired incretin effects in diabetes. Diabetes 56: 1551-1558 2007.

  • 10. Silva LS de Miranda AM de Brito Magalhães CL Dos Santos RC Pedrosa ML Silva ME. Diet supplementation with beta-carotene improves the serum lipid profile in rats fed a cholesterol-enriched diet. J Physiol Biochem 69: 811-820 2013.

  • 11. Anggrahini S Ika R Agnes M. Enrichment of β-carotene cassava flour noodles pumpkin (Cucurbita maxima Dutchenes). Article in Indonesian. Majalah Ilmu dan Teknologi Pertanian 26: 81-87 2006. http://www.jurnal-agritech.tp.ugm.ac.id/ojs/index.php/agritech/article/view/443.

  • 12. Caili F Huan S Quanhong L. A review on pharmacological activities and utilization technologies of pumpkin. Plant Foods Hum Nutr 61: 73–80 2006.

  • 13. Sedigheh A Moshtaghian SJ Setorki M et al. Hypoglycaemic and hypolipidemic effects of pumpkin (Cucurbita pepo L.) on alloxan-induced diabetic rats. Afr J Pharm Pharmacol 5: 2620-2626 2011.

  • 14. Wostmann BS. Nutrition and metabolism of the germfree mammal. World Rev Nutr Diet 22: 40-92 1975.

  • 15. Tranchida F Tchiakpe L Rakotoniaina Z Deyris V Ravion O Hiol A. Long-term high fructose and saturated fat diet affects plasma fatty acid profile in rats. J Zhejiang Univ Sci B 13: 307-317 2012.

  • 16. Ble-Castillo JL Aparicio-Trapala MA Juárez-Rojop IE et al. Differential effects of high-carbohydrate and high-fat diet composition on metabolic control and insulin resistance in normal rats. Int J Environ Res Public Health 9: 1663-1676 2012.

  • 17. Song Y Manson JE Tinker L et al. Insulin sensitivity and insulin secretion determined by homeostasis model assessment (HOMA) and risk of diabetes in a multiethnic cohort of women: the Women’s Health Initiative Observational Study. Diabetes Care 30: 1747–1752 2007.

  • 18. Paiva SA Russell RM. β-Carotene and other carotenoids as antioxidants. J Am Coll Nutr 18: 426–433 1999.

  • 19. Ondrejovičová I Muchová J Mišľanová C Nagyová Z Ďuračková Z. Hypercholesterolemia oxidative stress and gender dependence in children. Prague Med Rep 111(4): 300–312 2010.

  • 20. Liu Z Stanojevic V Brindamour LJ Habener JF. GLP1-derived nonapeptide GLP1 (28-36)amide protects pancreatic β-cells from glucolipotoxicity. J Endocrinol 213: 143-154 2012.

  • 21. Holst JJ. The physiology of glucagon-like peptide 1. Physiol Rev 87: 1409-1439 2007.

  • 22. Vilsbøll T Agersø H Krarup T Holst JJ. Similar elimination rates of glucagon-like peptide-1 in obese type 2 diabetic patients and healthy subjects. J Clin Endocrinol Metab 88: 220-224 2003.

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