Gender and Contractile Functions of Slow and Fast Skeletal Muscles in Streptozotocin Induced Diabetic Sprague Dawley Rats

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Abstract

Objectives: Diabetes mellitus has been linked with specific morphological and metabolic abnormalities of skeletal muscle in a fiber specific manner. Aim: The present study was designed to compare the contractile functions of slow and fast skeletal muscles in streptozotocin (STZ) induced diabetic male and female Sprague Dawley rats. Material and methods: Thirty healthy Sprague Dawley rats (15 male and 15 female) were divided into two groups and studied after four weeks following diabetes induction. The rats in group I (male diabetic; n = 15) and group II (female diabetic; n = 15) were fed on normal pellet diet and water ad libitum and rendered diabetic by single intraperitoneal injection of STZ 65 mg/kg body weight at the start of study (day 1). At the end of four weeks, the contractile parameters of slow soleus and fast extensor digitorum longus (EDL) muscles were recorded by iWorx advanced animal/human physiology data acquisition unit (AHK/214). Results: At the end of four weeks, the weight of isolated soleus and EDL muscles in the male diabetic rats was significantly higher (p < 0.001) as compared to the female diabetic rats. However, no significant difference was found in any of the contractile functions of isolated soleus and EDL muscles when compared between the male and female diabetic rats. Conclusion: No gender differences exist in the contractile functions of slow and fast skeletal muscles in streptozotocin induced diabetic Sprague Dawley rats.

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  • 1. Bourey RE Koranyi L James DE Mueckler M Permutt MA. Effects of altered glucose homeostasis on glucose transporter expression in skeletal muscle of the rat. J Clin Invest 86: 542-547 1990.

  • 2. Bernroider E Brehm A Krssak M et al. The role of intramyocellular lipids during hypoglycemia in patients with intensively treated type 1 diabetes. J Clin Endocrinol Metab 90: 5559-5565 2005.

  • 3. Russell ST Rajani S Dhadda RS Tisdale MJ. Mechanism of induction of muscle protein loss by hyperglycaemia. Exp Cell Res 315:16-25 2009.

  • 4. Krause MP Riddell MC Hawke TJ. Effects of type 1 diabetes mellitus on skeletal muscle: clinical observations and physiological mechanisms. Pediatr Diabetes 12: 345-364 2011.

  • 5. Sexton WL Poole DC Mathieu-Costello O. Microcirculatory structure-function relationships in skeletal muscle of diabetic rats. Am J Physiol 266: H1502-H1511 1994.

  • 6. Weiss RB. Streptozotocin: A review of its pharmacology efficacy and toxicity. Canc Treat Rep 66: 427-438 1982.

  • 7. Ganda OP Rossini AA Like AA. Studies on streptozotocin diabetes. Diabetes 25: 595- 603 1976.

  • 8. Szkudelski T. The mechanism of alloxan and streptozotocin action in β Cells of the rat pancreas. Physiol Res 50: 537-546 2001.

  • 9. Leslie M Forger NG Breedlove SM. Sexual dimorphism and androgen effect on spinal motoneurons innervating the rat flexor digitorum brevis. Brain Res 561: 269-273 1991.

  • 10. Miller AE MacDougall JD Tarnopolsky MA Sale DG. Gender differences in strength and muscle fiber characteristics. Eur J Appl Physiol Occup Physiol 66: 254-262 1993.

  • 11. Cureton KJ Collins MA Hill DW McElhannon FM. Muscle hypertrophy in men and women. Med Sci Sports Exerc 20: 338-344 1988.

  • 12. Hicks AL Kent-Braun J Ditor DS. Sex differences in human skeletal muscle fatigue. Exerc Sport Sci Rev 29: 109-112 2001.

  • 13. Brotto M Brotto L Nosek TM Romani A. Temporal adaptive changes in contractility and fatigability of diaphragm muscles from streptozotocin-diabetic rats. J Biomed Biotech doi:10.1155/2010/931903 2010.

  • 14. Eng CM Smallwood LH Rainiero MP Lahey M Ward SR Lieber RL. Scaling of muscle architecture and fiber types in the rat hindlimb. J Exp Biol 211: 2336-2345 2008.

  • 15. Hu N Xie S Liu L et al. Opposite effect of diabetes mellitus induced by streptozotocin on oral and intravenous pharmacokinetics of verapamil in rats. Drug Metab Dispos 39: 419-425 2011.

  • 16. Dupouy VM Ferre PJ Uro-Coste E Lefebvre HP. Time course of COX-1 and COX-2 expression during ischemia-reperfusion in rat skeletal muscle. J Appl Physiol 100: 233-239 2006.

  • 17. Young AA Gedulin B Wolfe-Lopez D Greene HE Rink TJ Cooper JS. Amylin and insulin in rat soleus muscle: dose responses for co secreted non competitive antagonists. Am J Physiol 263: E274-E281 1992.

  • 18. Maas H Jaspers RT Baan GC Huijing PA. Myofascial force transmission between a single muscle head and adjacent tissues: length effects of head III of rat EDL. J Appl Physiol 95: 2004-2013 2003.

  • 19. Warmington SA Tolan R McBennett S. Functional and histological characteristics of skeletal muscle and the effects of leptin in the genetically obese (ob/ob) mouse. Int J Obes Relat Metab Disord 24: 1040-1050 2000.

  • 20. Harrison AP Clausen T. Thyroid hormone-induced up regulation of Na+ channels and Na+-K+ pumps: implications for contractility. Am J Physiol 274: R864-R867 1998.

  • 21. Clark KI White TP. Morphology of stable muscle grafts of rats: effects of gender and muscle type. Muscle Nerve 8: 99-104 1985.

  • 22. Ebuehi OA Ajuluchukwu AE Afolabi OT Akinwande AI. Oxidative stress in alloxan-induced diabetes in female and male rats. Adv Med Dent Sci 3: 71-75 2010.

  • 23. Rees DA Alcolado JC. Animal models of diabetes mellitus. Diabet Med 22: 359-370 2005.

  • 24. Chen V Ianuzzo CD. Dosage effect of streptozotocin on rat tissue enzyme activities and glycogen concentration. Can J Physiol Pharmacol 60: 1251-1256 1982.

  • 25. Armstrong RB Gollnick PD Ianuzzo CD. Histochemical properties of skeletal muscle fibres in streptozotocin-diabetic rats. Cell Tiss Res 162: 387-394 1975.

  • 26. Hegarty PV Rosholt MN. Effects of streptozotocin-induced diabetes on the number and diameter of fibres in different skeletal muscles of the rat. J Anat 133: 205-211 1981.

  • 27. Cantillon D Bradford A. Effects of age and gender on rat upper airway muscle contractile properties. J Gerontol A Biol Sci Med Sci 55: B396-B400 2000.

  • 28. Rodriguez-Cuenca S Pujol E Justo R et al. Sex-dependent thermogenesis differences in mitochondrial morphology and function and adrenergic response in brown adipose tissue. J Biol Chem 277: 42958-42963 2002.

  • 29. Justo R Frontera M Pujol E et al. Gender-related differences in morphology and thermogenic capacity of brown adipose tissue mitochondrial subpopulations. Life Sci 76: 1147-1158 2005.

  • 30. Colom B Alcolea MP Valle A Oliver J Roca P Garcia-Palmer FJ. Skeletal muscle of female rats exhibit higher mitochondrial mass and oxidative-phosphorylative capacities compared to males. Cell Physiol Biochem 19: 205-212 2007.

  • 31. Schmalbruch H Kamieniecka Z. Fiber types in the human brachial biceps muscle. Exp Neurol 44: 313-328 1974.

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