Genetic diversity among 13 northern populations of the declining coastal plant Eryngium maritimum L. at the northernmost extent of the species distribution range was studied using retrotransposon- based SSAP molecular markers. Diversity indices varied extensively among populations; some showing extremely low diversity whereas other populations exhibited moderate amounts of genetic variation. Differentiation among populations was highly variable as well. Interestingly, differentiation among northern populations was not influenced strongly by geographic distance. Closely situated populations were often more divergent than more distant populations suggesting other factors may be responsible for genetic structuring of E. maritimum populations. We propose that the following genetic and environmental factors combine together in a complex relationship to mould the present genetic structure of E. maritimum populations in this region: (1) historic biogeographical processes; (2) local environmental conditions at each site; (3) success of sexual reproduction and proportion of clonal propagation; (4) size of the population and influence of genetic drift; (5) level of fragmentation and isolation. Lastly, we suggest that the sustainable existence of Latvian populations is seriously threatened, unless recommended conservation measures are implemented.
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Acosta A. Carranza M. L. Izzi C. F. (2009). Are there habitats that contribute best to plant species. Biodivers. Conserv.18 1087–1098.
Andersone U. Druva-Lusite I. Ievina B. Karlsons A. Necajeva J. Samsone I. Ievinsh G. (2011). The use on nondestructive methods to assess a physiological status and conservation perspectives of Eryngium maritimum L. J. Coastal Conserv. 15 509–522.
Aviziene D. Pakalnis R. Sendzikaite J. (2008). Status of red-listed species Eryngium maritimum L. on the Lithuanian coastal dunes. In: Environmental Engineering. Proceedings of the 7th International Conference May 22–28 2008. Vilnius Gediminas Technical University pp. 22–28.
Bonin A. Nicole F. Pompanon F. Miaud C. Taberlet P. (2007). Population adaptive index:Anew method to help measure intraspecific genetic diversity and prioritize populations for conservation. Conserv. Biol. 21 697–708.
de Bruin A. Ibelings B. W. Van Donk E. (2003). Molecular techniques in phytoplankton research: From allozyme electrophoresis to genomics. Hydrobiologia491 47–63.
Clausing G. Vickers K. Kadereit J. W. (2000). Historical biogeography in a linear system: Genetic variation of Sea Rocket (Cakile maritima) and Sea Holly (Eryngium maritimum) along European coasts. Mol. Ecol. 9 1823–1833.
Curle M. Stabbetorp O. E. Nordal I. (2007). Eryngium maritimum biology of a plant at its northernmost localities. Nord. J. Bot. 24 617–628.
Eckert C. G. (2002). The loss of sex in clonal plants. Evol. Ecol. 15 501–520.
Ellstrand N. C. Elam D. R. (1993). Population genetic consequences of small population size: Implications for plant conservation. Annu. Rev. Ecol. Evol. Syst. 24 217–242.
Excoffier L. Lischer H. E. L. (2010). Arlequin suite ver 3.5:Anew series of programs to perform population genetics analyses under Linux and Windows. Mol. Ecol. Resour. 10 564–567.
Flavell A. J. Dunbar E. Anderson R. Pearce S. R Hartley R. Kumar A. (1992). Ty1-copia group retrotranspozons are ubiquitous and heterogenous in higher plants. Nucleic Acids Res. 20 3639–3644.
Frankham R. (2003). Genetics and conservation biology. Comptes Rendus Biol. 326 22–29.
Ievina B. Rostoks N. Ievinsh G. (2009). Analysis of genetic diversity of Latvian population of Sea Holly Eryngium maritimum L. [Jûrmalas zilpodzes Eryngium maritmum L. Latvijas populâcijas ìençtiskâs daudzveidîbas analîze] Latvijas Veìetâcija 18 13–24 (in Latvian).
Ievina B. Syed N. H. Flavell A. J. Ievinsh G. Rostoks N. (2010). Development of retrotransposon-based SSAP molecular marker system for study of genetic diversity in sea holly (Eryngium maritimum L.) Plant Genet. Resour. 8 258–266.
Iserman M. Rooney P. (2014). Biological flora of British Isles: Eryngium maritimum. J. Ecol. 102 789–821.
Kadereit J. W. Arafeh R. Somogyi G. Westberg E. (2005). Terrestrial growth and marine dispersal? Comparative phylogeography of five coastal plant species at a European scale. Taxon54 861–876.
Kumar A. Bennetzen J. L. (1999). Plant retrotransposons. Annu Rev. Genet. 33 479–532.
Łabuz T. A. (2007). Evaluation of past and present Sea Holly (Eryngium maritimum) habitats on Polish coastal dunes. Acta Univ. Latv. 723 99–114.
Mantel N. (1967). The detection of disease clustering and a generalized regression approach. Cancer Res. 27 209–220.
Minasiewicz J. Borzyszkowska S. Żółkoś K. Bloch-Orłowska J. Afranowicz R. (2011). Population genetic structure of the rare species Eryngium maritimum L. (Apiaceae) on the Gulf of Gdañsk: Implications for conservation and management. Biodiv. Conserv. 24 39–48.
Necajeva J. Ievinsh G. (2013). Seed dormancy and germination of an endangered coastal plant Eryngium maritimum (Apiaceae). Estonian J. Ecol. 62 150–161.
Nei M. (1972). Nei’s original measures of genetic identity and genetic distance. Amer. Nat. 106 283–292.
Nei M. (1973). Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA70 3321–3323.
Nei M. (1987). Molecular Evolutionary Genetics. 1st Ed. Columbia University Press New York. 512 pp.
Olšauskas A. M. Urbonienė R. (2008). State of Eryngium maritimum L. population on the Curonian Spit coastal dunes. Environ. Res. Eng. Man. 2 69–74.
Syed N. H. Flawell A. J. (2006). Sequence-specific amplification polymorphisms (SSAPs):Amulti-locus approach for analyzing transposon insertions. Nat. Protoc. 1 2746–2752.
Vos P. Hogers R. Bleeker M. Reijans M. van de Lee T. Hornes M. Frijters A. Pot J. Peleman J. Kuiper M. Zabeau M. (1995). AFLP: a new technique forDNAfingerprinting. Nucleic Acids Res23 4407–4414.
Wright S. (1931). Evolution in Mendelian populations. Genetics16 97–159.
Zdot;ółkoś K. Afranowicz R. Bloch-Orlowska J. Koziel K. (2007). Distribution and the resources of Sea Holly (Eryngium maritimum L.) on the western shore of the Gulf of Gdansk. Biodiv. Res. Conserv. 5 55–60.