Genetic and Agronomic Analysis of Latvian Fescue (Festuca Spp.), Ryegrass (Lolium Spp.) Accessions and their Hybrids


The development of ecologically adaptable fodder crop varieties is of increasing importance, particularly in the context of climate change. New varieties should be phenotypically and ecologically plastic and able to adapt to differing climactic and soil conditions, ensuring high yields and persistence. Combining Festuca and Lolium species and the development of hybrid (Festulolium) cultivars can be a promising method of combining high yield, high feed quality, persistence, as well as cold, frost and drought tolerance. Breeders at the Institute of Agriculture of Latvia University of Life Sciences and Technologies have been utilizing Festulolium germplasm for several decades. Currently, in cooperation with the molecular genetics laboratory and Latvian gene bank at the Latvian State Forest Research Institute “Silava”, analysis of Festuca, Lolium and their hybrids with DNA markers has been initiated, in order to gain additional knowledge about the breeding material and to increase the efficiency of the breeding process. Results of the assessment of morphological and agronomic traits in long-term field trials are combined with DNA markers analyses in order to determine the correlation of genetic and phenotypic traits.

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  • Becker, T., Isselstein, J., Benke, M., Kayser, M. (2018). Persistence of modern varieties of Festuca arundinacea L. and Phleum pratense L. as an alternative to Lolium perenne L. in intensively managed sown grasslands. In: Sustainable Meat and Milk Production from Grasslands. Proceedings of the 27th General Meeting of the European Grassland Federation, Cork, Ireland, 17–21 June 2018. Teagasc, Animal & Grassland Research and Innovation Centre, pp. 117–119.

  • Berzins, P., Jansone, S., Rancane, S., Stesele, V., Dzene, I. (2015). The evaluation of perennial grass cultivars in Latvia condition. In: Proceedings of the 25th NJF Congress “Nordic View to Sustainable Rural Development”, Riga, Latvia, 16–18 June 2015, pp. 141–147.

  • Berzins, P., Rungis, D., Rancane, S., Gailite, A., Belevica, V., Stesele, V., Vezis, I., Jansons A. (2018a). Yield and genetic composition of Latvian × Festulolium cultivars and breeding material. In: Brazauskas, G. et al. (eds.). Breeding Grasses and Protein Crops in the Era of Genomics. Springer, pp. 62–67.

  • Berzins, P., Rancane, S., Stesele, V., Vezis, I. (2018b). Performance of Lolium spp., Festuca spp. and their mutual hybrids in Latvian conditions. Sustainable meat and milk production from grasslands. In: Horan, B., Hennessy, D., O’Donovan, M. et al. (eds.). Proceedings of the 27th General Meeting of the European Grassland Federation, Cork, Ireland 17–21 June 2018, pp. 123–126.

  • Casler, M. D., Peterson, P. R., Hoffman, L. D., Ehlke, N. J., Brummer, E. C., Hansen, J. L., Mlynarek, M. J., Sulc, M. R., Henning, J. C., Undersander, D. J., Pitts, P. G., Bilkey, P. C., Rose-Fricker, C. A. (2002). Natural selection for survival improves freezing tolerance, forage yield and persistence of Festulolium. Crop Sci., 42, 1421–1426.

  • Cougnon M., Baert J., Van Waes C., Reheul D. (2013). Performance and quality of tall fescue (Festuca arundinacea Schreb.) and perennial ryegrass (Lolium perenne L.) and mixtures of both species grown with or without white clover (Trifolium repens L.) under cutting management. Grass and Forage Sci., 69, 666–677.

  • Dieringer, D., Schlötterer, C. (2003). Microsatellite Analyser (MSA): A platform independent analysis tool for large microsatellite data sets. Mol. Ecol. Notes3 (1), 167–169.

  • Earl, D. A., vonHoldt, B. M. (2012). STRUCTURE harvester: A website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv. Gen. Res., 4 (2), 1–3.

  • Lemežienė, N., Kanapeckas, J., Tarakanovas, P., Nekrošas, S. (2004). Analysis of dry matter yield structure of forage grasses. Plant Soil Environ., 50, 2004 (6), 277–282.

  • Moser, I. E., Buxton, D. R., Casler, M. D. (eds.) (1996). Cool Season Forage Grasses. Agron. Monogr., Vol. 34. American Society of Agronomy, Crop Science Society of America, Soil Science Society of America, Madison, Wisconsin. 841 pp.

  • Østrem, L., Volden, B., Larsen, A. (2013a). Morphology, dry matter yield and phenological characters at different maturity stages of Festulolium compared with other grass species. Acta Agricult. Scand., Section B. Soil Plant Sci., 63 (6), 531–542.

  • Østrem, L., Novoa-Garrid,o M., Larsen, A. (2013b). Festulolium: An interesting forage grass for high-latitude regions? Grassland Sci. Eur., 18, 270–272.

  • Peakall, R., Smouse, P. E. (2006). GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes6 (1), 288–295.

  • Porebski, S., Bailey, L. G, Baum, B. R. (1997). Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol. Biol. Rep., 15, 8–15.

  • Porras-Hurtado, L., Ruiz, Y., Santos, C., Phillips, C., Carracedo, Į., Lareu, M. V. (2013). An overview of STRUCTURE: Applications, parameter settings, and supporting software. Frontiers Genet., 4, 98.

  • Pritchard, J. K., Stephens, M., Donnelly, P. (2000). Inference of population structure using multilocus genotype data. Genetics55 (2), 945–959.

  • Studer, B., Asp, T., Frei, U., Hentrup, S., Meally, H., Guillard, A., Barth, S., Muylle, H., Roldán-Ruiz, I., Barre, P., Koning-Boucoiran, C., Uenk-Stunnenberg, G., Dolstra, O., Skøt, L., Skøt, K. P., Turner, L. B., Humphreys, M. O., Kölliker, R., Roulund, N., Nielsen, K. K., Lübberstedt, T. (2008). Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenne L.). Mol. Breed., 21 (4). 533–548.

  • Thomas, H., Humphreys, M. O. (1991). Progress and potential of interspecific hybrids of Lolium and Festuca. Agr. Sci., 117, 1–8.

  • Thomas, H. M., Morgan, W. G., Humphreys, M. W. (2003). Designing grasses with a future — combining the attributes of Lolium and Festuca. Euphytica133, 19–26.

  • Wilkins, P. W., Humphreys, M. O. (2003). Progress in breeding perennial forage grasses for temperate agriculture. J. Agr. Sci., 140, 129–150.


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