Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia

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Since its discovery in 2005, human bocavirus 1 (HBoV1) has globally been one of the most common respiratory viruses. It is currently accepted that HBoV1 is a pathogen, causing upper and lower respiratory tract infections (LRTIs) in children. However, due to the prolonged HBoV1 DNA shedding from the upper airways and the subsequent high rate of co-detections with other respiratory viruses, the interpretation of positive polymerase chain reaction results is challenging. The aim of this study was to identify acute HBoV1 infections by the presence of HBoV1-specific IgM and IgG measured by competition enzyme immunoassay, to elucidate the induction of Th1/Th2 cytokines, and to describe the clinical characteristics associated with acute HBoV1 infection in hospitalised children less than five years of age with LRTI. HBoV1 IgM was detected in 19/102 (18.6%) and IgG in 66/102 (64.7%) patients. HBoV1 IgM was most frequently found in patients aged 13 to 24 months. Pneumonia and acute wheezing were the most common clinical diagnoses among HBoV1 IgM positive patients. The seroprevalence of HBoV1-specific IgG increased with age, reaching 85% by the age of five years. INF-γ, IL-4, IL-5, and IL-10 were observed to be higher in patients with acute HBoV1 infection.

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  • Allander T. Tammi M. T. Eriksson M. Bjerkner A. Tiveljung-Lindell A. Anderson B. (2005). Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc. Nat. Acad. Sci. USA 102 (36) 12891–12896.

  • Allander T. Jartti T. Gupta S. Neisters H. G. M. Lehtinen P. Osterback R. Vuorinen T. Waris M. Bjerknen A. Tiveljung-Lindell A. van der Hoogen B. G. Hyypiä T. Russkanen O. (2007). Human bocavirus and acute wheezing in children. Clin. Infect. Dis. 44 (7) 904–910.

  • Anonymous (1995). The management of acute respiratory infections in children: Practical Guidelines for Outpatient Care. World Health Organization Geneva. 75 pp. (at p. 9).

  • Bastien N. Brandt K. Dust K. Ward D. Li Y. (2006). Human Bocavirus infection Canada. Emerg. Infect. Dis. 12 (5) 848–850.

  • Blessing K. Neske F. Herre U. Kreth H. W. Weissbrich B. (2009). Prolonged detection of human bocavirus DNA in nasopharyngeal aspirates of children with respiratory tract disease. Pediatr. Infect. Dis. J. 28 (11) 1018–1019.

  • Christensen A. Nordbø S. A. Kroksta S. Rognlien A. G. Døllner H. (2010). Human bocavirus in children: Mono-detection high viral load and viraemia are associated with respiratory tract infection. Clin. Virol. J. 49 (3) 158–162.

  • Chung J. Y. Han T. H. Kim J. S. Kim S. W. Park C. G. Hwang E. S. (2008). Th1 and Th2 cytokine levels in nasopharyngeal aspirates from children with human bocavirus bronchiolitis. J. Clin. Virol.43 (2) 223–225.

  • Contopoulos-Ioannidis D. G. Seto I. Hamm M. P. Thomson D. Hartling L. Ioannidis J. P. Curtis S. Constantin E. Batmanabane G. Klassen T. Williams K. (2012). Empirical evaluation of age groups and age-subgroup analyses in pediatric randomized trials and pediatric meta-analyses. Pediatrics129 (3) 161–184.

  • Deng Y. Gu X. Zhao X. Luo J. Luo Z. Wang L. Fu Z. Yang X. Liu E. (2012). High viral load of human bocavirus correlates with duration of wheezing in children with severe lower respiratory tract infection. PLoS One7 (3) e34353.

  • Don M. Söderlund-Venermo M. Valent F. Lahtinen A. Hedman L. Canciani M. Hedman K. Korppi M. (2010). Serologically verified human bocavirus pneumonia in children. Pediatric Pulmon.45 (2) 120–126.

  • Edner N. Castillo-Rodas P. Falk L. Hedman K. Söderlund-Venermo M. Allander T. (2012). Life-threatening respiratory tract disease with human bocavirus-1 infection in a 4-year-old child. J. Clin. Microbiol.50 (2) 531–532.

  • El-Radhi A.S Barry W. (2006). Thermometry in paediatric practice. Arch. Dis. Childhood91 (4) 351–356.

  • Endo R. Ishiguro N. Kikuta H. Teramoto S. Shirkoohi R. Ma X. Ebihara T. Ishiko H. Ariga T. (2007). Seroepidemiology of human bocavirus in Hokkaido prefecture Japan. J. Clin. Microbiol.45 (10) 3218–3223.

  • Eskola V. Xu M. Söderlund-Venermo M. (2017). Severe lower respiratory tract infection caused by human bocavirus 1 in an infant. Pediatric Infect. Dis. J. 36 (11) 1107–1108.

  • Foulongne V. Olejnik Y. Perez V. Elaerts S. Rodičre M. Segondy M. (2006). Human bocavirus in French children. Emerging Infect. Dis.12 (8) 1251–1253.

  • Fry A. M. Lu X. Chittaganpitch M. Peret T. Fischer J. Dowell S. F. Anderson L. J. Erdman D. Olsen S. J. (2007). Human bocavirus: A novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand. J. Infect. Dis. 195 (7) 1038–1045.

  • Guo L. Wang Y. Zhou H. Wu C. Song J. Li J. Paranhos-Baccalą G. Vernet G. Wang J. Hung T. (2012). Differential seroprevalence of human bocavirus species 1-4 in Beijing China. PLoS One7 (6) e39644.

  • Guido M. Tumolo M. R. Verri T. Romano A. Serio F. De Giorgi M. De Donno A. Bagordo F. Zizza A. (2016). Human bocavirus: Current knowledge and future challenges. World J. Gastroenterol.22 (39) 8684–8697.

  • Hirose Y. Hamada H. Wakui T. Ogawa T. Terai M. (2015). Characteristic systemic cytokine responses in children with human bocavirus-positive lower respiratory tract infection. Microbiol. Immunol.58 (3) 215–218.

  • Jartti T. Hedman K. Jartti L. Ruuskanen O. Allander T. Söderlund-Venermo M. (2012). Human bocavirus — the first five years. Rev. Med. Virol.22 (1) 46–64.

  • Jula A. Waris M. Kantola K. Peltola V. Söderlund-Venermo M. Hedman K. Ruuskanen O. (2013). Primary and secondary human bocavirus 1 infections in a family Finland. Emerg. Inf. Dis. 19 (8) 1328–1331.

  • Kahn J. S. Kesebir D. Cotmore S. F. D'Abramo A. Cosby C. Weibel C. Tattersall P. (2008). Seroepidemiology of human bocavirus defined using recombinant virus-like particles. J. Infect. Dis. 198 (1) 41–50.

  • Karalar L. Lindner J. Schimanski S. Kertai M. Segerer H. Modrow S. (2010). Prevalence and clinical aspects of human bocavirus infection in children. Clin. Microbiol. Infect.16 (6) 633–639.

  • Kantola K. Hedman L. Allander T. Jartti T. Lehtinen P. Ruuskanen O. Hedman K. Söderlund-Venermo M. (2008). Serodiagnosis of human bocavirus infection. Clin. Infect. Dis.46 (4) 540–546.

  • Kantola K. Hedman L. Arthur J. Alibeto A. Delwart E. Jartti T. Ruuskanen O. Hedman K. Söderlund-Venermo M. (2011). Seroepidemiology of human bocaviruses 1-4. J. Infect. Dis. 204 (9) 1403–1412.

  • Kantola K. Hedman L. Tanner L. Simell V. Makinen M. Partanen J. Sadeghi M. Veijola R. Knip M. Ilonen J. Hyoty H. Toppari J. Simell O. Hedman K. Söderlund-Venermo M. (2015). B-cell responses of human bocavirus 1-4: New insights from a childhood follow-up. PLoS One10 (9) 1–12.

  • Kesebir D. Vazquez M. Weibel C. Shapiro E.D. Ferguson D. Landry M. L. Kahn J. S. (2006). Human bocavirus infection in young children in the United States: Molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus. J. Infect. Dis. 194 (9) 1276–1282.

  • Körner R. W. Söderlund-Venermo M. van Koningsbruggen-Rietschel S. Kaiser R. Malecki M. Schildgen O. (2011). Severe human bocavirus infection Germany. Emerg. Infect. Dis. 17 (12) 2303–2305.

  • Kumar A. Filippone C. Lahtinen A. Hedman L. Söderlund-Venermo M. Hedman K. Franssila R. (2011). Comparison of Th-cell immunity against human bocavirus and parvovirus B19: Proliferation and cytokine responses are similar in magnitude but more closely interrelated with human bocavirus. Scand. J. Immunol.73 (2) 135–140.

  • Manning A. Russell V. Eastick K. Leadbetter G. H. Hallam N. Templeton K. Simmonds P. (2006). Epidemiological profile and clinical associations of human bocavirus and other humanparvoviruses. J. Inf. Dis. 194 (9) 1283–1290.

  • Martin E. T. Fairchok M. P. Kuypers J. Margaret A. Zerr D. M. Wald A. Englund J. A. (2010). Frequent and prolonged shedding of bocavirus in young children attending daycare. J. Inf. Dis. 201 (11) 1625–1632.

  • Martin E. T. Kuypers J. McRoberts J. P. Englund J. A. Zerr D. M. (2015). Human bocavirus 1 primary infection and shedding in infants. J. Inf. Dis. 212 (4) 516–524.

  • Meriluoto M. Hedman L. Tanner L. Simell V. Mäkinen M. Simell S. Mykkänen J. Korpelainen J. Ruuskanen O. Ilonen J. Knip M. Simell O. Hedman K. Söderlund-Venermo M. (2012). Association of human bocavirus 1 infection with respiratory disease in childhood follow-up study Finland. Emerg. Infect. Dis. 18 (2) 264–271.

  • Moesker F. M. van Kamper J. J. van der Eijk A. A. van Rossum A. M. de Hoog M. Schutten M. Smits S. L. Bodewes R. Osterhaus A. D. Fraaij P. L. (2015). Human bocavirus infection as a cause of severe acute respiratory tract infection in children. Clin. Microbiol. Infect.21 (10) 964.

  • Nascimento-Carvalho C. M. Cardoso M. R. Meriluoto M. Kemppainen K. Kantola K. Ruuskanen O. Hedman K. Söderlund-Venermo M. (2012). Human bocavirus infection diagnosed serologically among children admitted to hospital with community-acquired pneumonia in a tropical region. J. Med. Virol. 84 (2) 253–258.

  • Nascimento-Carvalho A. C. Vilas-Boas A. L. Fontoura M. H. Xu M. Vuorinen T. Söderlund-Venermo M. Ruuskanen O. Nascimento-Carvalho C. M. PNEUMOPAC-Efficacy Study Group. (2018). Sero-logically diagnosed acute human bocavirus 1 infection in childhood community-acquired pneumonia. Pediatric Pulmonol.53 (1) 88–94.

  • Nora-Krūkle Z. Rasa S. Vilmane A. Grāvelsiņa S. Kālis M. Ziemele I. Naciute M. Petraitiene S. Mieliauskaite D. Klimantavicience M. Girkontaite I. Liu H. F. Lin J. H. Lin Y. C. Chan H. C. Gardovska D. Murovska M. (2015). Presence of human bocavirus 1 in hospitalized children with acute respiratory tract infections in Latvia and Lithuania. Proc. Latvian Acad. Sci. Section B70 (4) 198–204.

  • Pavia A. T. (2011). Viral infections of the lower respiratory tract: Old viruses new Vvruses and the role of diagnosis. Clin. Infect. Dis. 52 (4) 284–289.

  • Qui J Söderlund-Venermo M Young N. S. (2017). Human Parvoviruses. Clin. Microbiol. Rev. 30 (1) 43–113.

  • Roth D. E. Caulfield L. E. Ezzati M. Black R. E. (2008). Acute lower respiratory infections in childhood: Opportunities for reducing the global burden through nutritional interventions. Bull. World Health Org.86 (5) 356–364.

  • Söderlund-Venermo M. Lahtinen A. Jartti T. Hedman L. Kemppainen K. Lehtinen P. Allander T. Ruuskanen O. Hedman K. (2009). Clinical assessment and improved diagnosis of bocavirus-induced wheezing in children Finland. Emerg. Inf. Dis. 15 (9) 1423–1430.

  • Ursic T. Steyer A. Kopriva S. Kalan G. Kriver U. Petrovec M. (2011). Human bocavirus as the cause of a life-threatening infection. J. Clin. Microbiol. 49 (3) 1179–1181.

  • Ursic T. Krivec U. Kalan G. Petrovec M. (2015). Fatal human bocavirus infection in an 18-month-old child with chronic lung disease of prematurity. Pediatric Infect. Dis. J. 34 (1) 111–112.

  • von Linstow M. L. Høgh M. Høgh B. (2008). Clinical and epidemiologic characteristics of human bocavirus in Danish infants. Pediatric Infect. Dis. J. 27 (10) 897–902.

  • Xu M. Arku B. Jartti T. Koskinen J. Peltola V. Hedman K. Söderlund-Venermo M. (2017). Comparative diagnosis of human bocavirus 1 respiratory infection with messenger RNA reverse-transcription polymerase chain reaction (PRC) DNA quantitative PCR and serology. J. Infect. Dis.215 (10) 1551–1557.

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