Assimilation of Selenium, Copper, and Zinc in Rye Malt


Trace elements selenium, copper, and zinc are essential minerals for the human body. One of the means to increase the micronutrient content in diets is to add them to food raw materials, for example, to enrich grain with micronutrients during malt production. To obtain rye malt, 3 kg grain was soaked in 10 l water with addition of three mineral salts — sodium selenate (Na2SeO4), copper sulphate (CuSO4 5H2O), and zinc sulphate (ZnSO4 7H2O) at different concentrations and different combination of salts. The concentration of selenium, copper, and zinc was determined in rye malt. The obtained results were used to calculate the degree of assimilation of trace elements in rye malt. The interaction of trace elements selenium/copper and copper/zinc was studied. The total amount of selenium, copper, and zinc was analysed by inductively coupled plasma mass spectrometry (ICP-MS). Sample preparation for elemental analysis was performed by wet acid digestion in a closed microwave mineralisation system. The selenium assimilation degree in rye malt was within 10.6 to 12.2%. Accordingly, copper assimilation was 32.8 to 38.0% and zinc 49.3 to 57.0%. Simultaneous presence of selenium/copper and copper/zinc during rye grain soaking promoted the assimilation of each mineral, compared with only one mineral additive.

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  • Brown, K. H., Wuehler, S. E., Peerson, J. M. (2011).The importance of zinc in human nutrition and estimation of the global prevalence of zinc deficiency. Food Nutr. Bull., 22 (2), 113–125.

  • Buligin, S. J., Demishev, L. F., Doronin, V. A., Zarisnak, A. S., Pascenko, J. V., Turovskii, J. E., Fateev, A. I., Jakovenko, M. M., Kordin, A. I. (2007). Microelements in Agriculture [Булыгин, С. Ю., Демишев, Л. Ф., Доронин, В. А., Заришняк, А. С., Пащенко, Я. В., Туровский, Ю. Е., Фатеев, А. И., Яковенко М. М., Кордин А. И. Микроэлементы в сельском хозяйстве]. Sich, Dnepropetrovsk. 100 pp. (in Russian).

  • Butzen, S. (2010). Micronutrients for Crop Production. Crop Insights, 20 (9), 2–4.

  • Dūma, M. (2010). The qualitative evaluation of grain fortified with selenium. Doctoral thesis. Latvia University of Agriculture, Jelgava, pp. 50–95.

  • Khoshgoftarmanesh, A. H., Schulin, R., Chaney, R. L., Daneshbakhsh, B., Afyuni, M. (2010). Micronutrient-efficient genotypes for crop yield and nutritional quality in sustainable agriculture: A review. Agron. Sustain. Dev., 30, 83–107.

  • Korzeniowska, J., Stanisławska-Glubiak, E. (2011).The effect of foliar application of copper on content of this element in winter wheat grain. Polish J. Agron., 4, 3–6.

  • Lintshinger, J., Fuchs, N., Moser, H., Jäger, R., Hlebeina, T., Markolin, G., Gössler, W. (1997). Uptake of various trace elements during germination of wheat, buckwheat and quinoa. Plant Food Human Nutr., 50, 223–237.

  • Lohry, R. (2007). Micronutrients: Functions, Sources and Application Methods. In: Indiana CCA Conference Proceedings. Sioux City, Iowa.

  • Malasarn, D., Kropat, J., Hsieh, S. I., Finazzi, G., Casero, D., Loo, J. A., Pellegrini, M., Wollman, F., Merchant, S. S. (2013). Zinc deficiency impacts CO2 assimilation and disrupts copper homeostasis in Chlamydomonasreinhardtii. J. Biol. Chem., 288 (15), 10672–10683.

  • Morgounov, A., Gómez-Becerra, H. F., Abugalieva, A., Dzhunusova, M., Yessimbekova, M., Muminjanov, H., Zelenskiy, Y., Cakmak, I. (2007). Iron and zinc grain density in common wheat grown in Central Asia. Int. J. Plant Breed., 155 (1), 193–203.

  • Mousavi, S. R., Galavi, M., Rezaei, M. (2013). Zinc (Zn) Importance for crop production: Review. Int. J. Agron. Plant Prod., 4 (1), 64–68.

  • Orlov, D. S. (1998). Microelements in soil and live organism [Орлов, Д. С. Микроэлементы в почвах и живых организмах]. Soros Education Journal [Соросовский образовательный журнал],, 1, 61–68 (in Russian).

  • Tamas, M., Mandoki, Z., Csapo J. (2010). The role of selenium content of wheat in the human nutrition. A literature review. Acta Univ. Sapientiae, Alimentaria, 3, 5–34.

  • Terry, N., Zayed, A. M., de Souza, M. P., Tarun, A. S. (2000). Selenium in higher plants. Annu. Rev. Plant Physiol. Mol. Biol., 51, 401–432.

  • Titov, A. F., Talanova, V. V., Kaznina, N. M., Laidinen, G. F. (2007). The Resistance of Plants to Heavy Metals [Титов, А. Ф., Таланова, В. В., Казнина, Н. М., Лайдинен, Г. Ф. Устойчивость растений к тяжелым металлам]. Karelskii Scientific Centre of Russian Academy of Sciences, Petrozavodsk. 172 pp. (in Russian).

  • Wang, Y., Wang, X., Wong, Y. (2012). Proteomics analysis reveals multiple regulatory mechanisms in response to selenium in rice. J. Proteomics, 75 (6), 1849–1866.

  • Właúniewski, S., Kaniuczak, J., Hajduk, E., Nazarkiewicz M. (2014). Influence of limiting and mineral fertilization on the copper content in grain of spring barley (Hordeum vulgare L.) and winter wheat (Triti Cumaestivum L.) cultivated on loessial soil. J. Elem., 821–832.


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