Botrytis cinerea Pers.:Fr. is a widespread necrotrophic pathogen causing grey mould on many economically important horticultural crops. The variability in various B. cinerea populations is known to be very high. Despite the economic importance, the variability of B. cinerea has not been investigated previously on fruit crops in Lithuania. The aim of the study was to characterise the variability of B. cinerea strains isolated from strawberry and apple in different growth conditions on various agar media and to assess mycelial compatibility among the isolates. Larger colony diameter after four days of incubation was observed for isolates from strawberry on potato dextrose and beer universal agars in 24 h dark or light regime, followed by pectin agar in 24 h light. Similarly, the maximum radial growth of the isolates from apple was on potato dextrose agar (dark), followed by beer universal agar (dark and light), after four days of incubation at 20 °C. In the mycelial compatibility tests, barrage formation was evident in mycelial contacts between several isolates, indicating their vegetative incompatibility. The tests revealed that 76% were compatible and 24% were incompatible among investigated strains.
If the inline PDF is not rendering correctly, you can download the PDF file here.
Beever R. E. Parkes S. L. (2003). Use of nitrate non-utilizing (Nit) mutants to determine vegetative compatibility in Botryotinia fuckeliana (Botrytis cinerea). Eur. J. Plant Pathol. 109 607-613.
Burgess T. Bihon W. Wingfield M. J. Wingfield B. D. (2009). A simple and rapid method to determine vegetative compatibility groups in fungi. Inoculum: Newsletter of the Mycological Society of America 60 (6) 1-2.
Burgess T. Wingfield M. J. Wingfield B. D. (2001). Comparison of genotypic diversity in native and introduced populations of Sphaeropsis sapinea isolated from Pinus radiata. Mycol. Res. 105 (11) 1331-1339.
Droby S. Wisniewski M. Macarisin D. Wilson C. (2009). Twenty years of postharvest biocontrol research: Is it time for a new paradigm? Postharvest Biol. Tec. 52 137-145.
Fernandez J. G. Fernandez-Baldo M. A. Sansone G. Calvente V. Benuzzi D. Salinas E. Raba J. Sanz M. I. (2014). Effect of temperature on the morphological characteristics of Botrytis cinerea and its correlated with the genetic variability. J. Coastal Life Med. 2 543-548.
Fillinger S. Elad Y. (2016). Botrytis - the Fungus the Pathogen and its Management in Agricultural Systems. Springer International Publishing AG. 478 pp.
Fournier E. Giraud T. Albertini A. Brygoo Y. (2005). Partition of the Botrytis cinerea complex in France using multiple gene genealogies. Mycologia 97 1251-1267.
Korolev N. Elad Y. Katan T. (2008). Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants. Eur. J. Plant Pathol.122 369-383.
Kumari S. Tayal P. Sharma E. Kapoor R. (2014). Analyses of genetic and pathogenic variability among Botrytis cinerea isolates. Microbiol. Res. 169 (11) 862-872.
Lecompte F. Abro M. A. Nicot P. C. (2010). Contrasted responses of Botrytis cinerea isolates developing on tomato plants grown under different nitrogen nutrition regimes. Plant Pathol. 59 (5) 891-899.
Liaudanskas M. Viškelis P. Kviklys D. Raudonis R. Janulis V. (2015). A Comparative Study of Phenolic Content in Apple Fruits. Int. J. Food. Prop. 18 (5) 945-953.
Malama A. A. Mironova S. N. Filimonova T. V. Moiseev Yu. V. Gumargalieva K. Z. (1985). The effect of temperature on growth of mycelial fungi colonies [Малама А. А. Миронова С. И. Влияние температур на рост колоний мицелярных грибов]. Mikrobiologia IМикробиологияJ 54 (6) 994–997 (in Russian).
Martinez F. Blancard D. Lecomte P. Levis C. Dubos B. Fermaud M. (2003). Phenotypic differences between vacuma and transposa subpopulations of Botrytis cinerea. Eur. J. Plant Pathol. 109 479-488.
Papaioannou I. A. Typas M. A. (2015). Barrage formation is independent from heterokaryon incompatibility in Verticillium dahliae. Eur. J. Plant Pathol. 141 (1) 71-82.
Rasiukevičiūtė N. (2016). Genetic and phenotypic diversity of Botrytis spp. from strawberry and onion diseases forecasting and control. Doctoral dissertation Lithuanian Research Centre for Agriculture and Forestry Akademija Lietuva.
Rasiukevičiūtė N. Valiuškaitë A. Survilienë-Radzevièë E. Supronienë S. (2013). Investigation of Botrytis cinerea risk forecasting model in strawberries. Proc. Latv. Acad. Sci. Section B 67 (2) 195-198.
Rasiukevičiūtė N. Valiuškaitë A. Uselis N. Buskienë L. Viðkelis J. Lukðienë Þ. (2015). New non-chemical postharvest techologies reducing berry contamination. Zemdirbytse-Agriculture 104 (4) 411-416.
Rigotti S. Viret O. Gindro K. (2006). Two new primers highly specific for the detection of Botrytis cinerea Pers.:Fr. Phytopathol. Mediterr. 45 253-260.
Sehajpal P. K. Singh P. J. (2014). Effect of temperature on growth sporulation and sclerotial formation of the fungus Botrytis gladiolorum Timm. in different culture media and standardization of inoculum load of the fungus for generation of disease. Int. J. Res. 1 772-779.
Survilienė-Radzevičė E. Rasiukevièiûtė N. Valiuškaitė A. Kazlauskaitė S. Duchovskienė L. (2013). Evaluation of iMETOS®sm Botrytis cinerea and Botrytis squamosa forecasting models in different agro-ecological regions. Rural Development 2013: Proceedings 6 (2) 250-253.
Uselis N. Lanauskas J. Valiuškaitė A. Viškelis P. (2009). Investigation of strawberry cultivars growing them in profiled soil under agrofilm. Sodininkystë ir Darþininkystë 28 (4) 51-60.
Valiuškaitė A. Kviklienė N. Kviklys D. Lanauskas J. (2006). Post-harvest fruit rot incidence depending on apple maturity. Agron. Res. 4 (Special issue) 427-431.
Valiučkaitė A. Raudonis L. Survilienė E. (2008). Control of grey mould and white leaf spot in strawberry. Zemdirbyste-Agriculture 95 (3) 221-226.
Viskelis P. Rubinskienė M. Bobinaitė R. Dambrauskienė E. (2010). Bioactive compounds and antioxidant activity of small fruits in Lithuania. J. Food Agric. Environ. 8 (3-4) 259-263.