Role of Interferon-Gamma (Ifn-γ) in Immune Response Regulation in Hiv-1 and Hiv-1 + Mycobacterium Tuberculosis (Tb) Infected Patients / Interferona-gamma (Ifn-γ) Loma Imūnās Atbildes Regulēšanā Pacientiem Ar HIV-1 un HIV-1 + mycobacterium Tuberculosis

Open access

Abstract

The aim of this research was to investigate the role of IFN-γ in interaction between IL-10, IL-18, IL-1b, CD4 cell counts and HIV-1 RNA viral load in the development of HIV-1 in patients co-infected with Mycobacterium tuberculosis (TB). The study was conducted by Rīga East Clinical University Hospital with data from the HIV-1 register, in collaboration with the RSU Joint Laboratory of Clinical Immunology and Immunogenetics. 200 HIV-1 infected patients and 184 HIV-1 with TB co-infection patients divided in four groups were included in the study. IFN-γ, IL-10, IL-18, IL-1b levels were measured in serum with commercially enzyme-linked immunosorbent assay (ELISA Vector-Best Corporation, Novosibirsk, Russia). CD4 cell counts were measured by flow Partec IVD cytometry (USA). HIV-1 RNA quantification was performed using the COBAS AmpliPrep/COBAS Taqman HIV-1 Test (Germany). All groups were compared with each another. IFN-γ production was significantly lower, and IL-10 and CD4 cell counts were significantly higher, in HIV-1 patients without TB compared with the other groups. The group with HIV-1 and TB had significantly elevated IL-18 production. HIV patients with primary TB had significantly elevated IFN-γ production and HIV-1 RNA viral load and significantly lower IL-10 production.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Anonymous (2013). Open Source Epidemiologic Statistic for Public Health. Available at: http://www.openepi.com/v37/Menu/OE_Menu.htm (accessed 25.11.2013).

  • Chen K. Kolls J. K. (2013). T Cell-Mediated Host Immune Defenses in the Lung. Ann. Rev. Immunol. 31 605-633.

  • Cheng S. M. Li J. C. B. Lin S. S. Lee D. C. W. Liu L. Chen Z. Lau A. S. Y. (2009). HIV-1 transactivator protein induction of suppressor cytokine signaling-2 contributes to dysregulation of IFN-g signaling. Blood 13 5192-5201.

  • Getahun H. Gunneberg C. Granich R. Nunn P. (2010). HIV infection- associated tuberculosis: The epidemiology and the response. Clin. Infect. Dis. 50 (Suppl. 3) S201-S207.

  • Boekhoudt G. H. McGrath A. G. Swisher J. F. Feldman G. M. (2015). Immune complexes suppress IFN-γ-induced responses in monocytes by activating discrete members of the SRC kinase family. J. Immunol. 194 (3) 983-989.

  • Klein S. A. Dobmeyer J. M. Dobmeyer T. S. Pape M. Ottmann O. G. Helm E. B. Hoelzer D. Rossol R. (1997). Demonstration of the Th1 to Th2 cytokine shift during the course of HIV-1 infection using cytoplasmic cytokine detection on single cell level by flow cytometry. AIDS 11 1111-1118.

  • Li J. C. B. Au K. Fang J. Yim H. C. H. Chow K. Ho P. Lau A. S. Y. (2011). HIV-1 trans-activator protein dysregulates IFN-g signaling and contributes to the suppression of autophagy induction. AIDS 25 15-25.

  • Modjarrad K. Vermund S. H. (2010). Effect of treating co-infections on HIV-1 viral load: A systematic review. Lancet Infect. Dis. 10 455-463.

  • Moller M. Hoal E. G. (2010). Current findings challenges and novel approaches inhuman genetic susceptibility to tuberculosis. Tuberculosis (Edinburg) 90 71-83.

  • Osakwe C. E. Bleotu C. Chifiriuc M. C. Crancea C. Otelea D. Paraschiv S. Petrea S. Dinu M. Baicus C. Streinu-Cercel A. Lazar V. (2010). TH1/TH2 cytokine levels as an indicator for disease progression in human immunodeficiency virus type 1 infection and response to antiretroviral therapy. Roum. Arch. Microbiol. Immunol. 69 (1) 24-34.

  • Pawlowski A. Jansson M. Sköld M. Rottenberg M. E. Källenius G. (2012). Tuberculosis and HIV co-infection. PLoS Pathog. 8 (2) e1002464.

  • Roff S. R Noon-Song E. N. Yamamoto J. K. (2013). The significance of interferon-g in HIV-1 pathogenesis therapy and prophylaxis. Frontiers Immunol. 4 498. Available at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3888948/ (accessed 20.05.2016).

  • Selwyn P. A. Hartel D. Lewis V. A. Schoenbaum E. E. Vermund S. H. Klein R. S. Walker A. T. Friedland G. H. (1989). A prospective study of the risk of tuberculosis among intravenous drug users with human immunodeficiency virus infection. New Engl. J. Med. 320 545-555.

  • Spellberg B. Edwards J. E. Jr. (2001). Type 1/type 2 immunity in infectious diseases. Clin. Infect. Dis. 32 (1) 76-102.

  • Young H. A. Hardy K. J. (1995). Role of interferon-gamma in immune cell regulation. J. Leukocyte Biol. 58 (4) 373-381.

  • Whalen C. Horsburgh C. R. Hom D. Lahart C. Simberkoff M. Eller J. (1995). Accelerated course of human immunodeficiency virus infection after tuberculosis. Amer. J. Respir. Crit. Care Med. 151 129-135.

Search
Journal information
Impact Factor


CiteScore 2018: 0.3

SCImago Journal Rank (SJR) 2018: 0.137
Source Normalized Impact per Paper (SNIP) 2018: 0.192

Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 465 186 5
PDF Downloads 249 130 5