Combined Effects of 50 Hz Electromagnetic Field and SiO₂ Nanoparticles on Oxidative Stress in Plant’s Gametic Cells / Zemfrekvences (50 Hz) Elekromagnētiskā Lauka Un SiO₂ Nanodaļiņu Kombinētā Ietekme Uz Augu Gametisko Šūnu Oksidatīvo Stresu

The paper presents the results of combined effects of an extremely low frequency electromagnetic field (50 Hz) (ELF EMF) and SiO₂ nanoparticles on fluorescence of plant gametic cells (immature microspores). The data were recorded by a BD FACSJazz^® cell sorter after cell irradiation by blue laser (488 nm). A significant difference of fluorescence was observed between gametic cells after 1 hour incubation in suspension of SiO₂ nanoparticles and the control gametic cells. It was observed that fluorescence intensity of gametic cell was higher with ELF EMF treatment in comparison to control cells, but it was statistically significant only for cells treated with electromagnetic radiation field with density 100 μT (ρ < 0.01) and 400 μT (ρ < 0.01). A different effect of ELF EMF were observed in cells incubated in SiO₂ nanoparticle suspension; interaction of the factors resulted in lower cell fluorescence in comparison to control cells. The present study showed that SiO₂ nanoparticles may be a source of oxidative stress, but jointly with 50 Hz electromagnetic field they can serve as an efficient antioxidant