Determination of Oxalate and Some Inorganic Anions in Green and Black Tea

Oxalate concentration differs in various daily consumed food products. The role of oxalic acid in the human body is very significant, as its compounds are responsible for the stability of biological membranes. However, insoluble calcium and magnesium oxalates can be accumulated in the body in the form of kidney stones. Oxalate concentration has been measured by high performance liquid, gas after derivatization and ion chromatography (IC). The most effective method for the simultaneous determination of oxalate and inorganic anions is ion chromatography with conductometric detection. Here, we report the results of the measurement of oxalic acid in bleak and green tea samples. Separation was performed by IC on an anion-exchange column Shodex IC SI-90 with surface-layer sorbent and conductimetric detection. The main analytical features of the method were: limit of detection of oxalic acid 0.03 mg/l, linear range 0.1-20 mg/l, correlation 0.9998, relative standard deviation 1%. The method did not need specific sample treatment and was successfully applied to the analysis of black and green tea samples. Oxalic acid was determined in the ranges 16.7-84 mg/l for green tea and 63-116 mg/l for black tea. Green tea contained lower oxalate ions concentration than black tea. The IC method has a lower detection limit for oxalate ions than HPLS and GC, ten and two times less, respectively