Occurrence of Stone Fruit Viruses in Plum Orchards in Latvia

Open access

To evaluate the occurrence of nine viruses infecting Prunus a large-scale survey and sampling in Latvian plum orchards was carried out. Occurrence of Apple mosaic virus (ApMV), Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV), and Plum pox virus (PPV) was investigated by RT-PCR and DAS ELISA detection methods. The detection rates of both methods were compared. Screening of occurrence of Strawberry latent ringspot virus (SLRSV), Arabis mosaic virus (ArMV), Tomato ringspot virus (ToRSV) and Petunia asteroid mosaic virus (PeAMV) was performed by DAS-ELISA. In total, 38% of the tested trees by RT-PCR were infected at least with one of the analysed viruses. Among those 30.7% were infected with PNRSV and 16.4% with PDV, while ApMV, ACLSV and PPV were detected in few samples. The most widespread mixed infection was the combination of PDV+PNRSV. Observed symptoms characteristic for PPV were confirmed with RT-PCR and D strain was detected. Comparative analyses showed that detection rates by RT-PCR and DAS ELISA in plums depended on the particular virus tested. The results obtained in this study revealed that commonly grown plum cultivars in Latvia are infected with economically important stone fruit viruses and highlight the need to implement a programme to produce and propagate virus-free planting material.

Anonymous (2004). PM 4/29 (1) Certification scheme for cherry. BulletinOEPP/ EPPO, 31, 447-462.

Barba, M., Hadidi, A., Candresse, T., Cambra, M. (2011). Plum pox virus. In: Hadidi, A., Barba, M., Candresse, T., Jelkmann, W. (eds.). Virus and Virus-like Diseases of Pome and Stone Fruits (pp. 185-197). St. Paul, Minnesota: APS Press.

Blystad, D. R., Munthe, T. (2006). Plum pox virus (PPV) in Norway. BulletinOEPP/EPPO Bulletin, 36, 212.

Cambra, M., Capote, N., Myrta, A., Llácer, G. (2006). Plum pox virus and estimated costs associated with sharka disease. Bulletin OEPP/EPPO Bulletin,36, 202-204.

Capote, N., Bertolini, E., Olmos, A., Vidal, E., Martínez, M. C., Cambra, M. (2009). Direct sample preparation methods for detection of Plum pox virus by real-time RT-PCR. Int. Microbiol., 12, 1-6.

Çevik, B., Yardimci, N., Çulal-Klllç, H. (2011). Detection of viruses infecting stone fruits in Western Mediterranean region of Turkey. Plant Pathol. J., 27 (1), 44-52.

Desvignes, J. C. (1999). Virus Diseases of Fruit Trees. Paris: Centre techniques interprofessionnel des fruits et legumes. 202 pp.

Diekmann, M., Putter, C. A. J. (1996). FAO/IPGRI technical guidelines for the safe movement of germplasm. In: Diekmann, M., Putter, C. A. J. (eds.). Stone Fruits, No. 16. (pp. 38-39). Rome: FAO Publication Division.

Domínguez, S., Aparicio, F., Sánchez-Navarro, J., Cano, A., Pallás, V., García-Brunton, J. (1998). Studies on the incidence of Ilarviruses and Applechlorotic leaf spot virus (ACLSV) in apricot trees in the Murcia region (Spain) using serological and molecular hybridization methods. ActaHort., 472, 203-210.

García, J. A., Cambra, M. (2007). Plum pox virus and Sharka disease. PlantViruses 1, 69-79.

Glasa, M., Candresse, T. (2005). Partial sequence analysis of an atypical Turkish isolate provides further information on the evolutionary history of Plum pox virus (PPV). Virus Res., 108, 199-206.

Glasa, M., Malinowski, T., Predajòa, L., Pûpola, N., Dekena, D., Michalczuk, L., Candresse, T. (2011). Sequence variability, recombinations analysis and specific detection of the W strain of Plum pox virus. Phytopathology, 101, 980-985.

Gümüș M., Paylan, I. C., Matic, S., Myrta, A.,. Sipahioglu, H. M., Erkan S. (2007). Occurrence and distribution of stone fruit viruses and viroids in commercial plantings of Prunus species in western Anatolia, Turkey. J. Plant Pathol., 89, 265-268.

Hadidi, A., Barba, M. (2011). Economic impact of pome and stone fruit viruses and viroids. In: Hadidi, A., Barba, M., Candresse, T., Jelkmann, W. (eds.). Virus and Virus-like Diseases of Pome and Stone Fruits (pp. 1-7). St. Paul, Minnesota: APS Press.

Hassan, M., Myrta, A., Polak, J. (2006). Simultaneous detection and identification of four pome fruit viruses by one-tube pentaplex RT-PCR. J. Virol. Meth., 133, 124-129.

Herrera, M., Madariaga, V. (2002). Incidencia de Prunus necrotic ringspotvirus (PNRSV), Prune dwarf virus (PDV), Tomato ringspot virus (ToRSV) Y Plum Pox Virus (PPV) en viveros de frutales de carozo de la zona central de Chile. Agr. Tecn., 62, 38-45.

Isac, M., Preda, S., Marcu, M. (1998). Aphid species-vectors of Plum poxvirus. Acta Virol., 42, 233-234.

Jarošová, J., Kumar, J. (2010). Simultaneous detection of stone fruit tree viruses by one-step multiplex RT-PCR. Sci. Hort., 125, 68-72.

Jarrar, S., Myrta, A., di Terlizzi, B., Savino, V (2001). Viruses of stone fruits in Palestine. Acta Hort., 550, 245-248.

Kārkliņš, J., Skrîvele, M., Kaufmane, E., Ikase, L. (2007). Plûmju ðíirnes [Plum Cultivars]. Dobele: Latvijas Valsts Augïkopîbas institûts. 204 lpp. (in Latvian).

Kaufmane, E., Ikase, L., Seglina, D. (2010). Pomological characteristics of dessert plum cultivars in Latvia. Acta Hort., 874, 337-341.

Kinard, G. R., Scott, S. W., Barnett, O. W. (1996). Detection of Applechlorotic leaf spot and Apple stem grooving virus using RT-PCR. PlantDis., 80, 616-621.

Kommineni, K. V., Gillett, J. M., Ramsdell, D. C. (1998).Astudy of Tobamoringspot virus and prune brown line resistant in twenty-five rootstockscion combination. HortTechnol., 8 (3), 349-353.

Labonne, G., Yvon, M., Quiot, J. B., Avinent, L., Llácer, G. (1995). Aphids as potential vectors of Plum pox virus: Comparison of methods of testing and epidemiological consequences. Acta Hort., 386, 207-218.

López, M., Llop, P., Olmos, A., Marco-Noales, E., Cambra, M., Bertolini, E. (2008). Are molecular tools solving the challenges posed by detection of plant pathogenic bacteria and viruses? Current Issues Mol. Biol., 11, 13-46.

Lovisolo, O. (1990). Ecological observations on Petunia asteroid mosaic virus (PeAMV). In: Proceedings of the First Symposium of the InternationalWorking Group on Plant Viruses with Fungal Vectors, 21-24 August 1990 (pp. 155-158). Braunschweig.

MacKenzie, D. J., McLean, M. A., Mukerji, S., Green, M. (1997). Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction. Plant Dis., 81, 222-226.

Malinowski, T., Michalczuk, L., Pûpola, N., Dçíena, Dz. (2012). Serological and molecular characterization of the isolates of plum pox virus found in Latvia. In: 22nd International Conference on Virus and Other Graft TransmissibleDiseases of Fruit Crops, 3-8 June 2012 (p. 151). Rome.

Massart, S., Brostaux, Y., Barbarossa, L., Cesar, V., Cieslinska, M., Dutrecq, O., Fonseca, F., Guillem, R., Lavina, A., Olmos, A., Steyer, S., Wetzel, T., Kummert, J., Jijakli, M. H. (2008). Inter-laboratory evaluation of a duplex RT-PCR method using crude extracts for the simultaneous detection of Prune dwarf virus and Prunus necrotic ringspot virus. Eur. J. PlantPathol., 122, 539-547.

Menzel, W., Jelkmann, W., Maiss, E. (2002). Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control. J. Virol. Meth., 99, 81-92.

Myrta, A., di Terlizzi, B., Pallas, V., Savino, V. (2002). Stato sanitario dell'albicocco nei paesi del bacino mediterraneo. Rivista di Frutticoltura edi ortofloricoltura, 64, 91-95.

Myrta, A., Di Terlizzi, B., Savino, V., Martelli, G. P. (2003). Virus diseases affecting the Mediterranean stone fruit industry: A decade of surveys. In: Myrta A., Di Terlizzi B., Savino V. (eds.). Virus and Virus-like Diseases ofStone Fruits, with Particular Reference to the Mediterranean Region. Opt. Mediterr., Ser. B Studies Res., 45, 15-23.

Myrta, A., Matic, S., Malinowski, T., Pasquini, G., Candresse, T. (2011) Applechlorotic leaf spot virus in stone fruit. In: Hadidi, A., Barba, M., Candresse, T., Jelkmann, W. (eds.). Virus and Virus-like Diseases of Pomeand Stone Fruits (pp. 85-90). St. Paul, Minnesota: APS Press.

Nemeth, M. (1986). Virus, Mycoplasma, and Rickettsia Diseases of FruitTrees. Budapest: Akadémiai Kiádo. 841 pp.

Olmos, A., Cambra, M., Dasí, M. A., Candresse, T., Esteban, O., Gorris, M. T., Asensio, M. (1997). Simultaneous detection and typing of plum poxpotyvirus (PPV) isolates by hemi-nested PCR and PCR-ELISA. J. Virol. Meth., 68, 127-137.

Pūpola, N., Moroèko-Bièevsk, I., Kâle, A., Zeltiòð, A. (2011). Occurrence and diversity of pome fruit viruses in apple and pear orchards in Latvia. J. Phytopathol., 159, 597-605.

Pūpola, N., Kâle, A., Gospodaryk, A. (2011). Âbeïu hlorotiskâ lapu plankumainîbas vîrusa izplatîba plûmju stâdîjumos [Occurrence of applechlorotic leaf spot virus in plum orchards]. In: Raþas svçtki „Vecauce -2011”: LLU mâcîbu un pçtîjumu saimniecîbai Vecauce - 90. Zinâtniskâseminâra rakstu krâjums (37.-41. lpp.). Jelgava: LLU (in Latvian).

Pūpola, N., Kâle, A., Jundzis, M., Moroèko-Bièevska, I. (2010). The occurrence of Ilarviruses in Latvian fruit orchards. Julius-Kühn-Archiv, 427, 263-267.

Roossinck, M. J., Bujarski, J., Ding, S. W., Hajimarad, R., Hanada, K., Scott, S., Tousignant, M. (2005). Bromoviridae. In: Fauquet, C. M., Mayo, M. A., Maniloff, J., Desselberger, U., Ball, L. A. (eds.). Virus Taxonomy. EightReport of the International Committee on Taxonomy of Viruses (pp. 1049-1058). Amsterdam: Elsevier/Academic Press.

Sánchez-Navarro, J. A., Aparicio, F., Herranz, M. C., Minafra, A., Myrta, A., Pallas, V. (2005). Simultaneous detection and identification of eight stone fruit viruses by one-step RT-PCR. Eur. J. Plant Pathol., 111, 77-84.

Sánchez-Navarro, J. A., Aparicio, F., Rowhani, A., Pallás, V. (1998). Comparative analysis of ELISA, nonradioactive molecular hybridization and PCR for the detection of Prunus necrotic ringspot virus in herbaceous and Prunus hosts. Plant Pathol., 47, 780-786.

Skrīvele, M., Kaufmane, E., Rubauskis, E., Ikase, L., Strautiòa, S., Ruisa, S., Blukmanis, M. (2008). Fruit and berry growing in Latvia. In: Proceedingsof International Scientific Conference „Sustainable Fruit Growing: FromPlant to Product”, 28-31 May 2008 (pp. 5-14). Jûrmala-Dobele, Latvia: Latvia State Institute of Fruit-growing.

Sochor, J., Babula, P., Adam, V., Krska, B., Rene, R. (2012). Sharka: The Past, the present and the future. Viruses, 4, 2853-2901.

Suchá J., Svobodová L. (2010). Incidence of Prune dwarf virus and Prunus necrotic ring spot virus in orchards of sweet and sour cherry in the Czech Republic. Hort. Sci. (Prague), 37, 118-120.

Torrance, L., Dolby, C. A. (1984). Sampling conditions for reliable routine detection by enzyme-linked immunosorbent assay of three ilarviruses in fruit trees. Ann. Appl. Biol., 104, 267-276.

Turka I., Ignaðs J., Miltiòð G., Kilçvica M. (1984). Nozîmîgâkâs kartupeïu,dârzeòu un augïu koku vîrusslimîbas un to ierobeþoðanas iespçjas Latvijâ [Significant Viral Diseases of Potato, Vegetables and Fruit Trees in Latvia and Posibilities of Their Control]. Jelgava: LLA rotaprints. 52 lpp. (in Latvian).

Uyemoto, J. K., Scott, S. W. (1992). Important diseases of Prunus caused by viruses and other graft-transmissible pathogens in California and South Carolina. Plant Disease, 76, 5-11.

Vidal, E., Moreno, A., Bertolini, E., Cambra, M. (2012a). Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models. Plant Pathol., 61, 413-422.

Vidal, E., Yokomi, R. K., Moreno, A., Bertolini, E., Cambra, M. (2012b). Calculation of diagnostic parameters of advanced serological and molecular tissue-print methods for detection of Citrus tristeza virus. A model for other plant pathogens. Phytopathology, 102, 114-121.

Verhoeven, J. Th. J., Haas, A. M. de, Roenhorst, J. W. (1998). Outbreak and eradication of Plum pox potyvirus in the Netherlands. Acta Hort., 472, 407-411.

Wetzel, T., Candresse, T., Ravelonandro, M., Dunez, J. (1991b). A polymerase chain reaction assay adapted to plum pox virus detection. J. Virol. Meth., 33, 355-365.

Wijkamp, I., Gaag, D. J. van der (2011). Pest Risk Analysis for Plum Pox Virus. Plant Protection Service, the Netherlands. 57 pp.

Journal Information

CiteScore 2017: 0.22

SCImago Journal Rank (SJR) 2017: 0.127
Source Normalized Impact per Paper (SNIP) 2017: 0.211


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 141 141 16
PDF Downloads 49 49 4