Toxicity and Genotoxicity Testing of Roundup

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Toxicity and Genotoxicity Testing of Roundup

Glyphosate, in the commercial formulation named Roundup, is a broad spectrum herbicide that is one of the most frequently applied pesticides in the world. However, there has been little evidence of Roundup toxicity or genotoxicity. Genotoxicity of glyphosate was carried out using the Escherichia coli SOS chromotest. The glyphosate-induced dose response in the SOS chromotest suggests that glyphosate possesses genotoxic properties. Glyphosate at a 0.2 g/l concentration in toxicity bioassay caused 50% mortality of Daphnia magna (LD50 after 24 h — 0.22 g/l; after 48 h — 0.19 g/l), but 0.25 — 0.5 g/l — 100% death of organisms (LD100 after 24 h — 0.5 g/l; after 48 h — 0.25 g/l). Our results (E. coli SOS chromotest and daphnia test system) together with recent animal studies and epidemiological reports suggest that glyphosate, especially, Roundup possesses both toxic and genotoxic properties.

Ahitiainen, J.H., Vanhala, P., Myllymaki, J. (2003). Effects of different plant protection programme on soil microbes. Ecotox. Environm. Safety, 54, 56--64.

Ames, B.N. (1992) Pollution, pesticides and cancer. J. AOAC (Association of Analytical Communities) Int., 75, 1--5.

Anonymous (1996). ISO 6341:20 Water quality: Determination of long time toxicity of substances to Daphnia magna Strauss (Cladocera Crustacea). International Organization for Standardization.

Blaustein, A.R., Johnson, P.T.J. (2003). Explaining frog deformities. Sci. Amer., Febr, 48--53.

Bolognesi, C., Bonatti, S., Dugan, P., Gallerani, E., Peluso, M., Rabboni, R., et al. (1997). Genotoxic activity of glyphosate and its technical formation Roundup. J. Agric. Food Chem., 47, 1957--1962.

Bolognesi, C. (2003). Genotoxicity of pesticides: A review of human biomonitoring studies. Mut. Res./Rev. Mut. Res., 543, pp. 251--272.

Brausch, J.M., Smith, P.N. (2007). Toxicity of three polyethylated tallowamine surfactant formulations to laboratory and field collected fairy shrimp, Thamnocephalus platyrus. Arch. Environ. Contam. Toxicol., 52(2), 217--221.

Bull, S., Fletcher K., Bodis, A.R., Battershill, J.M. (2006). Evidence for genotoxicity of pesticides in pesticide applicators: A review. Mutagenesis, 21, 93--103.

Colborn, T. A case for revisiting the safety of pesticides: A close look at neurodevelopment. http://www.medscape.com/viewarticle/522014?src=search

Cox, C. (1995). Glyphosate. Part 2: Human exposure and ecological effects. J. Pest. Reform, 15.

De Roos, A.J., Blair, A., Rusiecki, J.A., Hoppin, J.A., Svec, M., Dosemeci, M., Sandler, D.P., Alavanja, M.C. (2005). Cancer incidence among Glyphosate-exposed pesticide applications in the agricultural health study. Environ. Health Perspect., 113(1), 23--30.

Greenlee, A.R., Arbucle, T.E., Chyon, P.H. (2003). Risk factors for female infertility in an agricultural disturbances. Epidemiology, 14, 428--436.

Mesch-Sundermann, V., Mochayedi, S., Kevekordes, S. (1992). Genotoxicity of polycyclic hydrocarbons in Escherichia coli PQ37. Mut. Res., 278, 1--9.

Monroy, C.M., Cortes, A.C., Sicardi, D.M., de Restrepo, H.G. (2005). Cytotoxicity and genotoxicity of human cells exposure in vitro to glyphosate. Biomedica, 25(3), 335--345.

Quillardat, P., Hofnung, M. (1985). The SOS chromotest a colorimertric bacterial assay for genotoxins: procedures. Mut. Res., 147, 65--78.

Quillardat, P., Hofnung, M. (1993). The SOS chromotest: A review. Mut. Res., 297, 235--279.

Rank, J., Jensen, A.G., Skov, B., Pedersen, L.H., Jense, K. (1993). Genotxicity testing of the herbicide Roundup and its active ingredient glyphosate isopropilamine using the mouse bone marrow micronucleus test, Salmonella mutagenicity test, and Allium anaphase-telophase test. Mut. Res., 300(1), 29--36.

Richard S., Moeslemi S., Sipathur H., Benachour N., Seralini G. (2005). Differential effects of Glyophosate and Roundup on cells and aromatase. Environm. Health Perspect., 113, 716--720.

Service, R.F. (2007). A growing threat down on the farm. Science, 316, 1114--1117.

Stratton, G.V., Giles, J. (1990) Importance of bioassay volume in toxicity tests using algae and aquatic invertebrates. Envir. Contamin. Toxicol., 44(3), 420--427.

Tsui, M.T., Chu, L.M. (2003). Aquatic toxicity of glyphosate-based formulations: Comparison between different organisms and the effects of environmental factors. Chemosphere, 52(8), 1189--1197.

Williams, G.M., Kroes, R., Munro, I.C. (2000). Safety evaluation and risk assessment of the herbicide Roundup and its active ingredient, glyphosate, for humans. Regul. Toxicol. Pharmacol., 31(1), 117--165.

Клинцаре А.А (1983). Пестициды и микрофлора растений[Pesticides and Plant Microflora]. Рига, Зинатне. 168 с.

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