Optimised Routines for Monitoring of Treated Late Latent Syphilis Patients

Dzintars Ozoliņš 1 , Ilona Hartmane 2 , Ingmārs Mikažāns 2 , Daina Paegle 2 , Ināra Ančupāne 3  and Aija Žileviča 4
  • 1 Centre of Sexually Transmitted and Skin Diseases, Pauls Stradiņš Clinical Hospital, Pērnavas iela 70, Rīga, LV-1009, LATVIA
  • 2 Clinical Centre of Skin and Sexually Transmitted Diseases, A. Briāna iela 2, Rīga, LV-1001, LATVIA
  • 3 Children's Hospital of Rīga Stradiņš University, Vienības gatve 45, Rīga, LV-1004, LATVIA
  • 4 Faculty of Medicine, University of Latvia, Šarlotes iela 1a, Rīga, LV-1001, LATVIA

Optimised Routines for Monitoring of Treated Late Latent Syphilis Patients

Highly sensitive and specific tests are needed in monitoring of the serological activity and efficacy of treatment of late latent syphilis. In a retrospective study, the data of 52 patients who had received treatment for late latent syphilis were analysed in the Clinical Centre of Skin and Sexually Transmitted Diseases, Latvia. The diagnostic sensitivity and specificity was calculated by Syphilis Immunoblot IgG, Treponema Pallidum Immobilisation Reaction (TPIR) and Syphilis Express Diagnosis (SED). The correlation between Treponema Pallidum Immobilisation Reaction (TPIR) and Syphilis Immunoblot IgG, SED and TPIR results were estimated. Syphilis Immunoblot IgG is useful for latent syphilis diagnostics. The values of the synthetic peptide TmpA, TmpN15 and TmpN47 on the Syphilis Immunoblot IgG strip are useful for monitoring of the treatment of late latent syphilis patients.

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  • Bouis, D.A., Popova, T.G., Takashima, A., Norgard, M.V. (2001). Dendritic cells phagocytose and are activated by Treponemma pallidum. Infect. Immun., 69(1), 518-528.

  • Brinkman, M.B., McKevitt, M., McLoughlin, M., Perez, C., Howell, J., Weinstock, G. M., Norris, S.J., Palzkill, T. (2006). Reactivity of antibodies from syphilis patients to a protein array representing the Treponema pallidum proteome. J. Clin. Microbiol., 44(3), 888-891.

  • Ebel, A., Vanneste, L., Cardinaels, M., Sablon, E., Samson, I., De Bosshere, K., Hulstaert, F., Zrein, M. (2000). Validation of the INNO-LIA_ Syphilis as a confirmation assay for Treponema pallidum antibodies. Scand. J. Immunol., 38(215), 219-641.

  • Edward, W., Hook III, M.D., Peeling, R.W. (2004). Syphilis control—a continuing challenge. N. Eng. J. Med., 2, 351.

  • Eggers, C.H., Caimano, M.J., Clawson, M.L., Miller, W.G., Samuels, D.S., Radolf, J.D. (2002). Identification of loci critical for replication and compatibility of a Borrelia burgdorferi cp32 plasmid and use of a cp32-based shuttle vector for the expression of fluorescent reporters in the Lyme disease spirochete. Mol. Microbiol., 43, 281-295.

  • Fenton, K.A., Breban, R., Vardavas, R., Okano J.T., Martin, T., Aral, S., Blower, S. (2008). Infectious syphilis in high-income settings in the 21st century. Lancet Infect Dis., 8, 244-253.

  • Fraser, C. M., Norris, S. J., Weinstock, G. M., White, O., Sutton, G. G., Dodson, R., Gwinn, M., Hickey, E. K., Clayton, R., Ketchum, K. A., Sodergren, E., Hardham, J. M., McLeod, M. P., Salzberg, S., Peterson, J., Khalak, H., Richardson, D., Howell, J. K., Chidambaram, M., Utterback, T., McDonald, L., Artiach, P. Bowman, C., Cotton, M. D., Fujii, C., Garland, S., Hatch, B., Horst, K., Roberts, K., Sandusky, M., Weidman, J., Smith, H. O., Venter, J. C. (1998). Complete genome sequence of Treponema pallidum, the syphilis spirochete. Science, 281, 375-388.

  • Girons, I. S., Chi, B., Kuramitsu, H. (2000). Development of shuttle vectors for spirochetes. J. Mol. Microbiol. Biotechnol., 2, 443-445.

  • Gjestland, T. (1955). The Oslo study of untreated syphilis: An epidemiologic investigation of the natural course of the syphilitic infection based upon a re-study of the Boeck-Bruusgaard material. Acta Derm. Venereol., 35, 3-368.

  • Goh, B. T., Voorst Vader, P. C. (2001). European guideline for the management of syphilis. Int. Journal of STD&AIDS, 12 (Supplement 3), 26.

  • Haake, D. V. (2000). Spirochetal lipoproteins and pathogenesis. Microbiology, 146, 1491-1504.

  • Hagedorn, J., Vanneste, L., De Bosshere, K., Pottel, H., Hulstaert, F., Van de Voorde, A., Zrein, M. (1999). Validation of the interpretation algorithm of INNO-LIA_ Syphilis using well-characterized sera. Innogenetics NV, Gent, Belgium, Study Report code DR-SYP-009, 1-11.

  • Knight, C. S., Crum, M. A., Hardy, R. W. (2007). Evaluation of the LIAISON Chemiluminescence immunoassay for diagnosis of syphilis. Clin.Vaccine Immunol., 14(6), 710-713.

  • LaFond, R. E., Lukehart, S. A. (2006). Biological basis for syphilis. Clin Microbiol Rev, 19(1), 29-49.

  • Larson, S. A. (1995). Laboratory diagnosis and interpretation of tests for syphilis. Clin. Microbiol. Rev., 8, 1.

  • Lee, K. H., Choi, H. J., Lee, M. G., Lee, J. B. (2000). Virulent Treponema pallidum 47 kDa antigen regulates the expression of cell adhesion molecules and binding of T-lymphocytes to cultured human dermal microvascular endothelial cells. Yonsei Med. J., 41, 623-633.

  • Leslie, D. E., Azzato, F., Karapanagiotidis, T., Leydon, J., Fyfe, J. (2007). Development of a Real-Time PCR Assay to detect Treponema pallidum in clinical specimens and assessment of assay's performance by comparison with serological testing. J. Clin. Microbiol., 45(1), 93-96.

  • Lien, E., Sellati, T. J., Yoshimura, A., Flo, T. H., Rawadi, G., Finberg, R. W., Carroll, J. D., Espevik, T., Ingalls, R. R., Radolf, J. D., Golenbock, D. T. (1999) Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J. Biol. Chem. 274, 33419-33425.

  • Muller, I., Brade, V., Hagedorn, H., Straube, E., Schorner, C., Frosch, M., Hlobil, H., Stanek, G., Hunfeld, K. (2006) Is serological testing a reliable tool in laboratory diagnosis of syphilis? Meta-analysis of eight external quality control surveys performed by the German Infection Serology Proficiency Testing Program. J. Clin. Microbiol., 44(4), 1335-1341.

  • Ozoliņš, D., Hartmane, I., Dērveniece, A., Pakalne, V., Žileviča, A. (2006). Diagnostics of syphilis: Evaluation of the enzyme-linked immunosorbent assay of IgG and IgM in comparison with other methods of serological diagnostics. Proc. Latvian Acad. Sci., Section B, 60(4), 127-132.

  • Porcella, S. F., Schwan, T. G. (2001). Borrelia burgdorferi and Treponema pallidum: A comparison of functional genomics, environmental adoptions and pathogenic mechanisms. J. Clin. Invest., 107(6), 651-656.

  • Rompalo, A. M., Joesoef, M. R., O'Donnell, J. A., Augenbraun, M., Brady, W., Radolf, J. D., Johnson, R., Rolfs, R. T. (2001). Clinical manifestations of early syphilis by HIV status and gender: results of the syphilis and HIV study. Sex. Transm. Dis. 28, 158-165.

  • Smith, M. B., Hayden, R. T., Persing, D. H., Woods, G. L. (2001). Spirochete infections. In Clinical diagnosis and management by laboratory methods (pp. 1131-1144). Philadelphia; London; New York; St. Louis; Sydeny; Toronto: Henry J. B. Sounders Company.

  • Walker, E. M., Arnett, J. K., Heath, J. D., Norris, S. J. (1991). Treponema pallidum subsp. pallidum has a single, circular chromosome with a size of approximately 900 kilobase pairs. Infect. Immun. 59, 2476-2479.

  • Walker, E. M., Howell, J. K., You, Y., Hoffmaster, A. R., Heath, J. D., Weinstock, G. M., Norris, S. J. (1995). Physical map of the genome of Treponema pallidum subsp. pallidum (Nichols). J. Bacteriol., 177, 1797-1804.

  • Wesley, C. V., Barrett, L. K., Lukehart, S. A., Schmidt, B., Schriefer, M., Cameron, C. E. (2003). Serodiagnosis of syphilis: Antibodies to recombinant Tp0453, Tp92 and Gpd proteins are sensitive and specific indicators of infection by Treponema pallidum. J. Clin. Microbiol., 41(8), 3668-3674.

  • Woznicova, V., Smajs, D., Wechsler, D., Matejkova, P., Flasarova, M. (2007). Detection of Treponema pallidum subsp. pallidum from skin lesions, serum, and cerebrospinal fluid in an infant with congenital syphilis after clindamycin treatment of the mother during pregnancy. J. Clin. Microbiol., 45(2), 659-661.

  • Woznicova, V., Valisova, Z. (2007). Performance of CAPTIA SelectSyph-G Enzyme-Linked Immunosorbent Assay in syphilis testing of a high-risk population: Analysis of discordant results. J. Clin. Microbiol., 45(6), 1794-1797.