Isolation, identification and screening of Actinobacteria in volcanic soil of Deception Island (the Antarctic) for antimicrobial metabolites

Open access


This project aimed to isolate and characterize volcanic soil Actinobacteria from Deception Island, Antarctic. A total of twenty−four Actinobacteria strains were isolated using four different isolation media (Starch casein agar, R2 agar, Actinomycete isolation agar, Streptomyces agar) and characterized basing on 16S rRNA gene sequences. Tests for second− arymetabolites were performed using well diffusion method to detect antimicrobial activities against eight different pathogens, namely Staphyloccocus aureus ATCC 33591, Bacillus megaterium, Enterobacter cloacae, Klebsiella oxytoca, S. enterica serotype Enteritidis, S. enterica serotype Paratyphi ATCC 9150, S. enterica serotype Typhimurium ATCC 14028 and Vibrio cholerae. Antimicrobial properties were detected against Salmonella paratyphi A and Salmonella typhimurium at the concentration of 0.3092±0.08 g/ml. The bioactive strains were identified as Gordonia terrae, Leifsonia soli and Terrabacter lapilli. Results from this study showed that the soil of Deception Island is likely a good source of isolation for Actinobacteria. The volcanic soil Actinobacteria are potentially rich source for discovery of antimicrobial compounds.

ARENSKOTTER M., BROKER D. and STEINBUCHEL A. 2004. Biology of the metabolically diverse ge− nus Gordonia. Applied and Environmental Microbiology 70: 3195-3204.

CHUN J., LEE J.H., JUNG Y., KIM M., KIM S., KIM B.K. and LIM Y.W. 2007. EzTaxon: a web−based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Interna− tional Journal of Systematic and Evolutionary Microbiology 57: 2259-2261.


DRANCOURT M.,MCNEIL M.M., BROWN J.M., LASKER B.A.,MAURIN M., CHOUX M. and RAOUX D. 1994. Brain abscess due to Gordona terrae in an immunocompromised child: case report and review of infections caused by G. terrae. Clinical Infectious Disease 19: 258-262.

DU TOIT E.A. and RAUTENBACH M. 2000. A sensitive standardised micro−gel well diffusion assay for the determination of antimicrobial activity. Journal of Microbiology Methods 42: 159-165.

GRAMMER A. 1976. Antibiotic sensitivity and assay test. In: C.H. Collins and P.N. Lyne (eds) Micro− biological Methods Butterworths, London: 235 pp.

GRISOLD A.J.,ROLL P.,HOENIGL M., FEIERL G.,VICENZI−MOSER R. andMARTH E. 2007. Isolation of Gordonia terrae from a patient with catheter−related bacteraemia. Journal of Medical Micro− biology 56: 1687-1688.

KUBOTA K., KOMA D.,MATSUMIYA Y., CHUNG S.−Y. and KUBO M. 2008. Phylogenetic analysis of long−chain hydrocarbon−degrading bacteria and evaluation of their hydrocarbon−degradation by the 2,6−DCPIP assay. Biodegradation 19: 749-757.

LABEDA D.P. and SHEARER M.C. 1990. Isolation of actinomycetes for biotechnological applica− tions. In: D.P. Labeda (ed.) Isolation of Biotechnological Organisms from Nature. McGraw−Hill Inc, New York: 1-19.

LAZZARINI A., CAVALETTI L., TOPPO G. and MARINELLI F. 2000. Rare genera of actinomycetes as potential producers of new antibiotics. Antonie van Leeuwenhoek 78: 399-405.

LEE J.E., SEO J.P., LEE D.W.,KO Y.H. and LEE S.D. 2008. Terrabacter lapilli sp. nov., an actinomycete isolated from stone. International Journal of Systematic and Evolutionary Microbiology 58: 1084-1088.

MADHAIYAN M., POONGUZHALI S., LEE J.S., SENTHILKUMAR M., LEE K.C. and SUNDARAM S. 2010. Leifsonia soli sp. nov., a novel yellow pigmented actinobacterium isolated from teak rhizosphere soil. International Journal of Systematic and Evolutionary Microbiology 60: 1322-1327.

OKORO C.K., BROWN R., JONES A.L., ANDREWS B.A., ASENJO J.A., GOODFELLOW M. and BULL A.T. 2009. Diversity of culturable actinomycetes in hyper−arid soils of the Atacama Desert, Chile. Antonie van Leeuwenhoek 95: 121-133.

SANTELLA R.M. 2006. Approaches to DNA/RNA extraction and whole genome amplification. Can− cer Epidemiology, Biomarkers and Prevention 15: 1585-1587.

SHEN F.T., HO M.J., HUANG H.R., ARUN A.B., REKHA P.D. and YOUNG C.C. 2008. Molecular de− tection and phylogenetic characterization of Gordonia species in heavily oil−contaminated soils. Research in Microbiology 159: 522-529.

SOLANO G., ROJAS−JIMENEZ K., JASPARS M. and TAMAYO−CASTILLO G. 2009. Study of the diver− sity of culturable actinomycetes in the North Pacific and Caribbean coasts of Costa Rica. Antonie van Leeuwenhoek 96: 71-78.

SRIVIBOOL R. and SUKCHOTIRATANA M. 2006. Bioperspective of actinomycetes isolates from coastal soils: a new source of antimicrobial producers. Songklanakarin Journal of Science and Technology 28: 493-499.

STACKEBRANDT E., SMIDA J. and COLLINS M.D. 1988. Evidence of phylogenetic heterogeneity within the genus Rhodococcus: revival of the genus Gordona (Tsukamura). Journal of General and Applied Microbiology 34: 341-348.

THAKUR D., YADAV A., GOGOI B.K. and BORA T.C. 2007. Isolation and screening of Streptomyces in soil of protected forest areas from the states of Assam and Tripura, India, for antimicrobial metabolites. Journal of Medical Mycology 17: 242-249.

TSUKAMURA M. 1971. Proposal of a new genus, Gordona, for slightly acid−fast organisms occurring in sputa of patients with pulmonary disease and in soil. Journal of General Microbiology 68: 15-26.

ZARDAWI I.M., JONES F., CLARK D.A. and HOLLAND J. 2004. Gordonia terrae induced suppurative granulomatous mastitis following nipple piercing. Pathology 36: 275-280.

Polish Polar Research

The Journal of Committee on Polar Research of Polish Academy of Sciences

Journal Information

IMPACT FACTOR 2016: 0.636
5-year IMPACT FACTOR: 1.121

CiteScore 2016: 1.20

SCImago Journal Rank (SJR) 2015: 0.556
Source Normalized Impact per Paper (SNIP) 2015: 0.645


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 66 66 26
PDF Downloads 10 10 6