Genetic and epigenetic studies on populations of Deschampsia antarctica Desv. from contrasting environments on King George Island

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Genetic and epigenetic studies on populations of Deschampsia antarctica Desv. from contrasting environments on King George Island

Populations of Antarctic hairgrass Deschampsia antarctica Desv. from King George Island exhibit variation in many traits. The reason for that is not evident and could be addressed to variable environmental conditions. Obviously, phenotypic variation could be due to stable or temporal changes in expression pattern as the result of adaptation. Stable changes could be due to mutations or site DNA methylation variation that modified expression pattern. Recently, MetAFLP approach was proposed to study such effects. A variant of methylation sensitive AFLP (Amplified Fragment Length Polymorphism), based on the isoschizomeric combinations Acc65I/MseI and KpnI/MseI was applied to analyze the sequence and site DNA methylation differences between two D. antarctica populations exhibiting morphological dissimilarities. Both DNA sequence mutations and site methylation pattern alternations were detected among and within analyzed populations. It is assumed that such changes might have originated as the response to environmental conditions that induced site methylation alternations leading to phenotypic variation of D. antarctica populations from South Shetland Islands.

Alberdi M., Bravo L. A., Gutierrez A., Gidekel M. and Corcuera L. J. 2001. Ecophysiology of Antarctic vascular plants. Physiologia Plantarum 115: 479-486.

Bednarek P. T., Masojc P., Lewandowska R. and Mysków B. 2003. Saturating rye genetic map with amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers. Journal of Applied Genetics 44: 21-33.

Bednarek P. T., Orļowska R., Koebner R. M. D. and Zimny J. 2007. Quantification of the tissue-culture induced variation in barley (Hordeum vulgare L.). BMC Plant Biology. http://www.biomedcentral.com/1471-2229/7/10 http://www.biomedcentral.com/1471-2229/7/10

Bruce T. A., Matthes M. C., Napier J. A. and Pickett J. A. 2007. Stressful "memories" of plants: evidence and possible mechanisms. Plant Science 173: 603-608.

Cassells A. C. and Curry R. F. 2000. Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture. Implications for micropropagators and genetic engineers. Plant Cell Tissue and Organ Culture 64: 145-157.

Chinnusamy V. and Zhu J. K. 2009. Epigenetic regulation of stress response in plants. Current Opinion in Plant Biology 12: 133-139.

Chwedorzewska K. J. 2008. Poa annua L. in Antarctic - searching for the source of introduction. Polar Biology 31: 263-268.

Chwedorzewska K. J. and Bednarek P. T. 2008. Genetic variability in the Antarctic hairgrass Deschampsia antarctica Desv. from maritime Antarctic and sub-Antarctic sites. Polish Journal of Ecology 56: 209-216.

Chwedorzewska K. J., Bednarek P. T. and Puchalski J. 2004. Molecular and morphological variation of Antarctic grass Deschampsia antarctica Desv. from King George Island (Antarctica). Acta Societatis Botanicorum Poloniae 73: 23-29.

Chwedorzewska K. J., Gieļwanowska I., Szczuka E. and Bochenek A. 2008. Anatomical and genetic variation of Deschampsia antarctica Desv. from King George Island (the Antarctic). Polish Polar Research 4: 377-386.

Corner R. W. M. 1971. Studies in Colobanthus quitensis (Kunth) Bartl. and Deschampsia antarctica Desv.: IV. Distribution and reproductive performance in Argentine islands. British Antarctic Survey Bulletin 26: 41-50.

Cubas P., Vincent C. and Coen E. 1999. An epigenetic mutation responsible for natural variation in floral symmetry. Nature 401: 157-161.

Duchesne P. and Bernatchez L. 2002. AFLPOP: A computer program for simulated and real population allocation based on AFLP data. Molecular Ecology Notes 3: 380-383.

Finnegan E. J. 2001. Epialleles - a source of random variation in times of stress. Current Opinion in Plant Biology 5: 101-106.

Fowbert J. A. and Smith R. I. L. 1994. Rapid population increase in native vascular plants in the Argentine Is, Antarctic Peninsula. Arctic, Antarctic and Alpine Research 26: 290-296.

Frenot Y., Chown S. L., Whinam J., Selkirk P. M., Convey P., Skotnicki M. and Bergstrom D. M. 2005. Biological invasions in the Antarctic: extent, impacts and implications. Biological Reviews 80: 45-72.

Gieļwanowska I. 2003a. Responses of Deschampsia antarctica to abiotic stress factors. Polish Journal of Natural Science 1: 108-111.

Gieļwanowska I. 2003b. Ultrastructural investigations of Deschampsia antarctica mesophyll. In: M. Olech (ed.) The Functioning of Polar Ecosystems as Viewed Against Global Environmental Changes. XXXIX International Polar Symposium, Kraków: 47-49.

Grant-Downton R. T. and Dickinson H. G. 2005. Epigenetics and its implication for plant biology 1. The epigenetic network in plants. Annals of Botany 96: 188-195.

Grant-Downton R. T. and Dickinson H. G. 2006. Epigenetics and its implication for plant biology 2. The Epigenetic epiphany: Epigenetics, evolution and beyond. Annals of Botany 97: 11-27.

Halliburton R. 2004. Introduction to Population Genetics. Pearson Education Int., Upper Saddle River, New Jersey: 650 pp.

Hammer Ø., Harper D. A. T. and Ryan P. D. 2001. PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica 4: 9. http://palaeoelectronica.org/2001_1/past/issue1_01.htm 2001 http://palaeoelectronica.org/2001_1/past/issue1_01.htm

Heschel M. S., Sultan S. E., Glover S. and Sloan D. 2004. Population differentiation and plastic response to drought stress in the generalist annual Polygonum persicaria.International Journal of Plant Science 165: 817-824.

Holderegger R., Stehlik I., Lewis-Smith R. I. and Abbott R. J. 2003. Population of Antarctic hairgrass (Deschampsia antarctica) show low genetic diversity. Arctic, Antarctic and Alpine Research 35: 214-217.

Kaeppler S. M., Kaeppler H. F. and Rhee Y. 2000. Epigenetic aspects of somaclonal variation in plants. Plant Molecular Biology 43: 179-188.

Kappen L. 2000. Some aspects of the great success of lichens in the Antarctica. Antarctic Science 12: 314-324.

Kovařik A., Koukalová B., Bezděk M. and Opatrný Z. 1997. Hypermethylation of tobacco heterochromatic loci response to osmotic stress. Theoretical and Applied Genetics 95: 301-306.

Labra M., Ghiani A., Citterio S., Sgorbati S., Sala F., Vannini C., Ruffini-Castigilione M. and Baracale M. 2002. Analysis of cytosine methylation pattern in response to water deficit in pea root tips. Plant Biology 4: 694-699.

Lukens L. and Zhan S. 2007. The plant genome's methylation status and response to stress: implications for plant improvement. Current Opinion in Plant Biology 10: 317-322.

Madlung A. and Comai L. 2004. The effect of stress on genome regulation and structure. Annals of Botany 94: 481-495.

Nędzarek A. and Chwedorzewska K. J. 2004. Nutrients content in water flash chosen sites of Deschampsia antarctica (King George Island, Antarctica). Folia Universitatis Agriculture Stetinensis 234: 299-304.

Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.

Oleszczuk S., Zimny J. and Bednarek P. T. 2002. The application of the AFLP method to determine homozygous lines purity of barley (Hordeum vulgare L.). Cellular and Molecular Biology Letters 7: 777-784.

Peakall R. and Smouse P. E. 2001. GenALEx V5: Genetic Analysis in Excel. Population genetic software for teaching and research. Australian National University, Canberra. www.anu.edu.au/BoZo/GenALEx/

Pritchard J. K. and Wen W. 2003. Documentation for structure software: Version 2. Department of Human Genetics, University of Chicago, Chicago, IL. http://pritchbsduchicagoedu/software/readme_2_1/readmehtml http://pritchbsduchicagoedu/software/readme_2_1/readmehtml

Rout G. R., Mohapatra A. and Mohan Jain S. 2006. Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects. Biotechnology Advances 24: 531-560.

Shannon C. E. and Weaver W. 1949. The Mathematical Theory of Communication. University of Illinois Press, Urbana, IL: 117 pp.

Smith L. R. I. 2001. Plant colonisation response to climate change in the Antarctic. Folia Facultatis Scientiarium Naturalium Universitatis Masarykianae Brunensis Geographia 25: 19-33.

Smith L. R. I. 2003. The enigma of Colobanthus quitensis and Deschampsia antarctica in Antarctic. In: A. H. L. Huiskes, W. W. C. Gieskes, J. Rozema, R. M. L. Schorno, S. M. van der Vies and W. J. Wolff (eds) Antarctic Survey in a Global Context. Backhuys, Leiden: 234-239.

Stewards N., Ito M., Yamaguchi Y., Koizumi N. and Sano H. 2002. Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress. Journal of Biological Chemistry 277: 37741-37746.

Vos P., Hogers R., Bleeker M., Reijans M., van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M. and Zauber M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407-4414.

Wahl S., Ryser P. and Edwards P. J. 2001. Phenotypic plasticity of grass anatomy in response to light intensity and nutrient supply. Annals of Botany 88: 1071-1078.

van de Wouw M., van Dijk P. and Huiskes A. H. L. 2008. Regional genetic diversity in Antarctic hairgrass (Deschampsia antarctica Desv.). Journal of Biogeography 35: 365-376.

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