In this research two kinds of stimulation electrodes were compared in motor nerve conduction study: needle electrodes used in human medicine and electrodes made of injection needles connected to the stimulator via alligator-type electrodes. A study was conducted in 22 mixed-breed dogs. The resulting values of the potential amplitudes of the stimulus, the parameters of the complex muscle potentials, and the motor nerve conduction velocity were statistically compared. There was no statistical difference between the parameters obtained with the two types of stimulation electrodes. The results of our research constitute a basis for improving present-day procedures, improving aseptic procedures, reducing tissue trauma during research and lowering research costs due to the introduction of injection-needle electrodes and their benefits into the study of motor nerve conduction in animals.