Coagulology, biochemical profile and muscle pathology in calves diagnosed with nutritional muscular dystrophy

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The aim of this study was to explain the correlations between selenium deficiency, hemostatic and biochemical disorders, and the progression of pathological changes in calves diagnosed with nutritional muscular dystrophy (NMD). The study was performed on 20 calves with supplementation of 8 ml selenium and vitamin E preparation and 20 calves with symptoms of NMD. Blood was sampled from calves aged 5, 12 and 19 days. On day 19, samples of the biceps femoris muscle were collected from 6 animals in each group for histopathological analysis. The following blood parameters were determined: PLT, PT, TT, APTT, fibrinogen and D-dimer concentrations, antithrombin III activity, glucose, selenium and vitamin E concentrations, activity of CK, LDH and GSH-Px. Muscle sections were stained with H&E and HBFP. Platelet counts were significantly lower in calves with symptoms of NMD. No significant differences in coagulation parameters were observed between the groups. Sick calves were diagnosed with hyperglycemia and elevation of CK and LDH activity. Selenium and vitamin E concentrations in the blood serum were significantly lower in the experimental group together with significant drop in GSH-Px activity. Changes characteristic of Zenker’s necrosis were observed in a muscle of the sick animals. To our best knowledge this is the first study in which the attempt was made to explain the relationship between selenium deficiency and changes in the coagulation system in ruminants.

Abdulah R, Katsuya Y, Kobayashi K, Nakazawa M, Nara M, Murakami M, Koyama H (2007) Effect of sodium selenite supplementation on the levels of prostacyclin I(2) and thromboxane A(2) in human. Thromb Res 119: 305-310.

Abutarbush SM, Radostits OM (2003) Congenital nutritional muscular dystrophy in a beef calf. Can Vet J 44: 738-739.

Andres S, Mane MC, Sanchez J, Barrera B, Jimenez A (1996) Changes in GSHPx and muscle enzyme activities in lambs with nutritional myodegeneration following a single treatment with sodium selenite. Small Rumin Res 23: 183-186.

Arthur JR (2000) The glutathione peroxidases. Cell Mol Life Sci 57: 1825-1835.

Azzi A (2007) Molecular mechanism of alpha-tocopherol action. Free Radic Biol Med 43: 16-21.

Beytut E, Karatas F, Beytut E (2002) Lambs with white muscle disease and selenium content of soil and meadow hay in the region of Kars, Turkey. Vet J 163: 214-217.

Bienvenu T, Chadefaux B, Ankri A, Leblond V, Coude M, Salehian B, Binet JL, Kamoun P (1991) Antithrombin III activity is not related to plasma homocysteine concentrations. Haemostasis 21: 65-67.

Bostedt H, Schramel P (1990) The importance of selenium in the prenatal and postnatal development of calves and lambs. Biol Trace Elem Res 24: 163-171.

Eichler SD, Morrill JL, Velazco J (1997) Bioavailability of alpha-tocopherol fed with retinol and relative bioavailability of D-alpha-tocopherol of DL-alpha-tocopherol acetate. J Dairy Sci 80: 393-399.

Fontaine M, Valli VEO, Young LG (1977) Studies on vitamin E and selenium deficiency in young pigs IV. Effect on coagulation system. Can J Comp Med 41: 64-76.

Gentry PA, Ross ML, Hayatgheybi H (1994) Competency of blood coagulation in the newborn calf. Res Vet Sci 57: 336-342.

Gerloff BJ (1992) Effect of selenium supplementation on dairy cattle. J Anim Sci 70: 3934-3940.

Katz LM, O’Dwyer S, Pollock P (2009) Nutritional muscular dystrophy in a four-day-old Connemara foal. Ir Vet J 62: 119-124.

Knowles TG, Edwards JE, Bazeley KJ, Brown SN, Butterworth A, Warriss PD (2000) Changes in the blood biochemical and haematological profile of neonatal calves with age. Vet Rec 147: 593-598.

Lentz SR, Piegors DJ, Fernandez JA, Erger RA, Arning E, Malinow MR, Griffin JH, Bottiglieri T, Haynes WG, Heistad DD (2002) Effect of hyperhomocysteinemia on protein C activation and activity. Blood 100: 2108-2112.

Muth OH, Oldfield JE, Remmert LF, Schubert JR (1958) Effects of selenium and vitamin E on white muscle disease. Science 128: 1090.

Nishinaga M, Ozawa T, Shimada K (1993) Homocysteine, a thrombogenic agent, suppresses anticoagulant heparin sulfate expression in cultured porcine aortic endothelial cells. J Clin Invest 92: 1381-1386.

Pavlata L, Misurova L, Pechova A, Dvorak R (2011) The effect of inorganic and organically bound forms of selenium on glutathione peroxidase activity in the blood of goats. Vet Med-Czech 56: 75-81.

Pavlata L, Pechova A, Illek J (2001) Muscular dystrophy in dairy cows following a change in housing technology. Acta Vet Brno 70: 269-275.

Philippo M, Arthur JR, Price J, Halliday GJ (1987) The effects of selenium, housing and management on the incidence of pneumonia in housed calves. Vet Res 121: 509-512.

Saito T, Takenaka M, Miyai I, Yamamoto Y, Matsumura T, Nozaki S, Kang J (2001) Coagulation and fibrinolysis disorder in muscular dystrophy. Muscle Nerve 24: 399-402.

Sauls DL, Lockhart E, Warren ME, Lenkowski A, Wilhelm SE, Hoffman M (2006) Modification of fibrinogen by homocysteine thiolactone increases resistance to fibrinolysis: a potential mechanism of the thrombotic tendency in hyperhomocysteinemia. Biochemistry 45: 2480-2487.

Schneider C (2005) Chemistry and biology of vitamin E. Mol Nutr Food Res 49: 7-30.

Sheng XQ, Huang KX, Xu HB (2004) New experimental observation on the relationship of selenium and diabetes mellitus. Biol Trace Elem Res 99: 241-253.

Sobiech P, Radwińska J, Krystkiewicz W, Snarska A, Stopyra A (2008) Changes in the coagulation profile of cattle with left abomasal displacement. Pol J Vet Sci 11: 301-306.

Sobiech P, Zbanyszek M, Kuleta Z (2005) Coagulation profile of H-F cows. Universitatis Mariae Curie-Skłodowska Sectio D 19: 149-152.

Stapleton SR (2000) Selenium: an insulin-mimetic. Cell Mol Life Sci 57: 1874-1879.

Tunca R, Erdogan HM, Sozmen M, Citil M, Devrim AK, Erginsoy S, Uzlu E (2009) Evaluation of cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. Turk J Vet Anim Sci 33: 53-59.

Undas A, Stępień E, Plicner D, Zieliński L, Tracz W (2007) Elevated total homocysteine is associated with increased platelet activation at the site of microvascular injury: effects of folic acid administration. J Thromb Haemost 5: 1070-1072.

Żarczyńska K, Radwińska J, Snarska A, Rękawek W, Procajło A (2013) Disturbances in the acid-base and electrolyte balance and changes in serum mineral concentrations in calves diagnosed with nutritional muscular dystrophy. J Elem 18: 307-315.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

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