Morphology and immunoreactivity of canine and feline extramedullary plasmacytomas

Open access

Abstract

The aim of the study was the evaluation of morphology and immunophenotype of canine (19 cases) and feline (7 cases) extramedullary plasmacytomas. Tumours, located in skin, oral cavity and spleen were surgically excised, fixed and processed for histopathology and immunohistochemistry (CD79α, CD18, proliferating cell nuclear antigen, metallothionein). Histologically, tumours were classified into mature, cleaved, asynchronous, polymorphous blastic, hyalin, or monomorphous blastic type. All evaluated tumours showed cytoplasmic expression of CD79α antigen. The expression of CD18 was observed in canine cutaneous and splenic tumours. In canine tumours expression of metallothionein was low to moderate, while in feline plasmacytomas – absent or low. In canine tumours, the mitotic index and proliferating cell nuclear antigen index were positively correlated with the expression of metallothionein. In feline tumours no correlation between mitotic index, proliferating cell nuclear antigen and metallothionein was found. This is the first study describing expression of metallothionein in canine and feline extramedullary plasmacytoma.

Adelman L, Larson V, Sissener T, Sportswood T (2014) Extramedullary plasmacytoma in the lung of a Doberman pinscher dog. Can Vet J 55: 1237-1240.

Alexandrakis MG, Passam FH, Dambaki K, Passam AM, Nalbanti F, Stathopoulos ES, Kyriakou DS (2005) The assessment of proliferating cell nuclear antigen immunostaining in myelodysplastic syndromes and its prognostic significance. Eur J Histochem 49: 27-32.

Baer KE, Patnaik AK, Gilbertson SR, Hurvitz AI (1989) Cutaneous plasmacytomas in dogs: a morphologic and immunohistochemical study. Vet Pathol 26: 216-221.

Borgatti A (2010) Plasma cell tumors. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary hematology. Blackwell Publishing Ltd, USA, pp 516-518.

Brunnert SR, Altman NH (1991) Identification of immunoglobulin light chains in canine extramedullary plasmacytomas by thioflavine T and immunohistochemistry. J Vet Diagn Invest 3: 245-251.

Cangul IT, Wijnen M, Van Garderen E, van den Ingh TS (2002) Clinico-pathological aspects of canine cutaneous and mucocutaneous plasmacytomas. J Vet Med A Physiol Pathol Clin Med 49: 307-312.

Dincer Z, Jasani B, Haywood S, Mullins JE, Fuentealba IC (2001) Metallothionein expression in canine and feline mammary and melanotic tumours. J Comp Pathol 125: 130-136.

Gumulec J, Raudenska M, Adam V, Kizek R, Masarik M (2014) Metallothionein – immunohistochemical cancer biomarker: a meta-analysis PLoS One 9: e85346.

Gupta A, Fry JL, Meindel M, Gumber S (2014) Pathology in practice. Cutaneous extramedullary solitary digital plasmacytoma in a dog. J Am Vet Med Assoc 244: 163-165.

Ioachim E, Assimakopoulos D, Peschos D, Zissi A, Skevas A, Agnantis NJ (1999) Immunohistochemical expression of metallothionein in benign premalignant and malignant epithelium of the larynx: correlation with p53 and proliferative cell nuclear antigen. Pathol Res Pract 195: 809-814.

Majzoub M, Breuer W, Platz SJ, Linke RP, Hermanns W (2003) Histopathologic and immunophenotypic characterization of extramedullary plasmacytomas in nine cats. Vet Pathol 40: 249-253.

Mazzone A, Ricevuti G (1995) Leukocyte CD11/CD18 integrins: biological and clinical relevance. Haematologica 80: 161-175.

Moore PF, Affolter VK, Vernau W (2000) Immunophenotyping in the dog. In: Bonagura JD (ed) Kirk’s current veterinary therapy XIII: Small Animal Practice. WB Saunders Company, USA, pp 505-509.

Martano M, Carella F, Squillacioti C, Restucci B, Mazzotta M, Lo Muzio L, Maiolino P (2012) Metallothionein expression in canine cutaneous apocrine gland tumors. Anticancer Res 32: 747-752.

Morton LD, Barton CL, Elissalde GS, Wilson SR (1986) Oral extramedullary plasmacytomas in two dogs. Vet Pathol 23: 637-639.

Platz SJ, Breuer W, Pfleghaar S, Minkus G, Hermanns W (1999) Prognostic value of histopathological grading in canine extramedullary plasmacytomas. Vet Pathol 36: 23-27.

Ramos-Vara JA, Miller MA, Valli VE (2007) Immunohistochemical detection of multiple myeloma 1/interferon regulatory factor 4 (MUM1/IRF-4) in canine plasmacytoma: comparison with CD79a and CD20. Vet Pathol 44: 875-884.

Rannou B, Hélie P, Bédard C (2009) Rectal plasmacytoma with intracellular hemosiderin in a dog. Vet Pathol 46: 1181-1184.

Remmele W, Stegner HE (1987) Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue. Pathologe 8: 138-140.

Rowland PH, Linke RP (1994) Immunohistochemical characterization of lambda light-chain-derived amyloid in one feline and five canine plasma cell tumors. Vet Pathol 31: 390-393.

Schrenzel MD, Naydan DK, Moore PF (1998) Leukocyte differentiation antigens in canine cutaneous and oral plasmacytomas. Vet Dermatol 9: 33-41.

Simoes JP, Schoning P, Butine M (1994) Prognosis of canine mast cell tumors: a comparison of three methods. Vet Pathol 31: 637-647.

Tsirakis G, Pappa CA, Kaparou M, Katsomitrou V, Hatzivasili A, Alegakis T, Xekalou A, Stathopoulos EN, Alexandrakis MG (2011) Assessment of proliferating cell nuclear antigen and its relationship with proinflammatory cytokines and parameters of disease activity in multiple myeloma patients. Eur J Histochem 55: e21.

Valli VE, Jacobs RM, Parodi AL, Vernau W, Moore PF (2002) Histological classification of hematopoietic tumors of domestic animals, 2nd ed., American Registry of Pathology, Washington, DC, USA.

Van Wettere AJ, Linder KE, Suter SE, Olby NJ (2009) Solitary intracerebral plasmacytoma in a dog: microscopic, immunohistochemical, and molecular features. Vet Pathol 46: 949-951.

Walentowicz-Sadlecka M, Koper A, Galazka K, Koper K, Basta P, Mach P, Skret-Magierlo J, Dutsch-Wicherek M, Sikora J, Grabiec M, Kazmierczak W, Wicherek L (2013) The analysis of metallothionein immunoreactivity in stromal fibroblasts and macrophages in cases of uterine cervical carcinoma with respect to both the local and distant spread of the disease. Am J Reprod Immunol 70: 253-261.

Webb J, Chary P, Northrup N, Almy F (2008) Erythrophagocytic multiple myeloma in a cat. Vet Clin Pathol 37: 302-307.

Webster JD, Yuzbasiyan-Gurkan V, Miller RA, Kaneene JB, Kiupel M (2007) Cellular proliferation in canine cutaneous mast cell tumors: associations with c-KIT and its role in prognostication. Vet Pathol 44: 298-308.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

Journal Information


IMPACT FACTOR 2016: 0.697
5-year IMPACT FACTOR: 0.773

CiteScore 2016: 0.73

SCImago Journal Rank (SJR) 2016: 0.315
Source Normalized Impact per Paper (SNIP) 2016: 0.486

Cited By

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 420 415 52
PDF Downloads 303 303 32