Reference intervals for selected hematological and biochemical variables in Hucul horses

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Abstract

Background: Hucul horses are the unique, genetically distinct breed of Carpathian Mountains. Even though they are recognized as primitive breed, many morphological differences between them and other primitive horses have been reported. Neither hematological nor blood biochemical studies in this breed have been conducted so far.

Objectives: The aim of this study was to establish the reference intervals for basic hematological and selected biochemical variables and to compare them with other breeds.

Material and Methods: Blood samples were collected from 168 Hucul horses and the analyses were performed using routine methods. Mainly nonparametric method was used to establish reference intervals.

Results: The following reference intervals have been established (rounded to two significant digits): RBC: 7.0-13×1012/l; HGB: 106.1-195.8 g/l; HCT: 0.3-0.6 l/l; MCV: 35-50 fl; MCH 11.9-17.1 pg; MCHC: 31.9-34.8 g/dl; WBC: 7.5-22×109/l, bands: 0-0.5×109/l; segmented neutrophils: 3.3-10×109/l; eosinophils: 0-1.1×109/l; basophils: 0-0.3×109/l; lymphocytes: 1.9-12×109/l; monocytes: 0-0.2×109/l; PLT 95-350×109/l; MPV 5.2-7.0; ALP: 98-425 U/l; AST: 220-470 U/l; GGT: 9.1-31 U/l; total bilirubin: 6.5-29 μmol/l; CPK: 120-640 U/l; triglycerides: 0.1-0.9 mmol/l; urea: 3.8-11 mmol/l; creatinine: 44 -140 μmol/l; serum amyloid A: 130-5200 μg/l.

Conclusions: Hematological and biochemical variables in Hucul horses were closer to hot-blooded then to cold-blooded and primitive horses or wild equidae. The reference intervals presented in this study pose clinically useful tool for evaluation of blood check-up in Hucul horses.

Barrelet A, Ricketts S (2002) Haematology and blood biochemistry in the horse: a guide to interpretation. In Practice 24: 318-327.

Cebulj-Kadunc N, Bozic M, Kosec M,Cestnik V (2002) The influence of age and gender on haematological parameters in Lipizzan horses. J Vet Med A Physiol Pathol Clin Med 49: 217-221.

Clinical and Laboratory Standards Institute (2010) Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory; Approved Guideline. 3rd ed., Wayne, USA.

Cywinska A, Gorecka R, Szarska E, Witkowski L, Dziekan P, Schollenberger A (2010) Serum amyloid A level as a potential indicator of the status of endurance horses. Equine Vet J Suppl: 23-27.

Fornal A, Radko A, Piestrzyńska-Kajtoch A (2013) Genetic polymorphism of Hucul horse population based on 17 microsatellite loci. Acta Biochim Pol 60: 761-765.

Geffre A, Concordet D, Braun JP,Trumel C (2011) Reference Value Advisor: a new freeware set of macroinstructions to calculate reference intervals with Microsoft Excel. Vet Clin Pathol 40: 107-112.

Geiser DG, Goble DO, Held JP (1984) Normal hematology and serology of the Clydesdale draft horse. Equine pract 6: 7-9.

Georgescu SE, Manea MA, Dudu A, Costache M (2011) Phylogenetic relationships of the Hucul horse from Romania inferred from mitochondrial D-loop variation. Genet Mol Res 10: 4104-4113.

Grondin TD, Dewitt SF (2010) Normal hematology of the horse and donkey. In: Weiss DJ, Wardrop KJ (eds) Schalm’s Veterinary hematology. 6th ed., Wiley-Blackwell, Iowa, pp 821-829.

Harvey RB, Hambright MB, Rowe LD (1984) Clinical biochemical and hematologic values of the American Miniature Horse: reference values. Am J Vet Res 45: 987-990.

Hinchcliff KK, A. Geor R (2004) Equine sports medicine and surgery. 1st ed. Oxford, Saunders, pp 1294-1301.

Hulten C, Tulamo RM, Suominen MM, Burvall K, Marhaug G, Forsberg M (1999) A non-competitive chemiluminescence enzyme immunoassay for the equine acute phase protein serum amyloid A (SAA) – a clinically useful inflammatory marker in the horse. Vet Immunol Immunopathol 68: 267-281.

Kedzierski W (2011) Correlation of plasma creatine kinase activity and glucose level in exercised Purebred Arabian horses. Med Weter 67: 541-544.

Kedzierski W, Bergero D, Assenza A (2009) Trends of Hematological and Biochemical Values in the Blood of Young Race Horses during Standardized Field Exercise Tests. Acta Veterinaria (Beograd) 59: 457-466.

Kedzierski W, Kowalik S (2009) Leptin and Ghrelin and the Indices of Lipid Metabolism as Related to Sex Steroid Hormones in Trotters. J Equine Vet Sci 29: 17-23.

Kedzierski W, Pluta M (2013) The welfare of young Polish Konik horses subjected to agricultural workload. J Appl Anim Welf Sci 16: 35-46.

Knoepfli AB (2002) Exertional rhabdomyolysis in a 4-year-old standardbred filly. Can Vet J 43: 293-295.

Komosa M, Purzyc H (2009) Konik and Hucul horses: a comparative study of exterior measurements. J Anim Sci 87: 2245-2254.

Krumrych W, Wisniewski E, Danek J (1993) Haematological parameters of the Polish Primitive Horses. Arch Vet Pol 33: 205-216.

Kusza S, Priskin K, Ivankovic A, Jędrzejewska B, Podgórski T, Javor A, Mihok S (2013) Genetic characterization and population bottleneck in the Hucul horse based on microsatellite and mitochondrial data. Biol J Linn Soc 109: 54-65.

Mason I (1996) A World Dictionary of Livestock Breeds, Types and Varieties. 4th ed, C.A.B International, p 273.

Meyer DH, Harvey JW (2004) Veterinary laboratory medicine: interpretation and diagnosis. 3rd ed., Saunders, Philadelphia, pp 3-12.

Niedzwiedz A, Jaworski Z, Filipowski H, Zawadzki M, Wrzosek M, Sluzewska-Niedzwiedz M, Nicpon J (2013) Serum biochemical reference intervals for the Polish Konik horse (Equus cabalus gmelini Ant.). Vet Clin Pathol 42: 66-69.

Petersen HH, Nielsen JP, Heegaard PM (2004) Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res 35: 163-187.

Polish Horse Breeders Association, Breeding Statistics. http://pzhk.pl/hodowla/statystyka-hodowlana/

Price JS, Jackson B, Eastell R, Goodship AE, Blumsohn A, Wright I, Stoneham S, Lanyon LE, Russell RG (1995) Age related changes in biochemical markers of bone metabolism in horses. Equine Vet J 27: 201-207.

Purzyc H (2007) A general characteristic of Hucul horses. Acta Sci Pol – Medicina Veterinaria 6: 25-31.

Purzyc H, Kobrynczuk F, Bojarski J (2011) Sexual dimorphism in Hucul horses using discriminant analysis. Animal 5: 506-511.

Smith B (2009) Normal Values for Erythron Data, Normal Values for Leukogram Data In: Large animal internal medicine, 4th ed., Mosby Inc. p 1822.

Szarska E (1999) Badania laboratoryjne w treningu koni. Agencja Reklamowa „Crex”, Warszawa.

Trandzik JZ, R. Jakabova, D. Buleca, J. Massanyi, P. Hasko, M. Kozlik, P (2007) Genetic diversity of Hucul horse, based on microsatellite data in Slovak Republic. http://www.raumberg-gumpenstein.at/c/index.php?option=com_docman&task=doc_view&gid=2344&Itemid=100014

Tukey J (1977) Exploratory Data Analysis Pearson, 1st ed., Prinston University, USA, pp 120-127.

Walzer C (2003) Equidae. In: Fowler ME, Miller RE (eds) Zoo and wild animal medicine. 5th ed., Saunders, St. Louis, pp 578-586.

Winnicka A (2011) Wartości referencyjne podstawowych badań laboratoryjnych w weterynarii. 5th ed., Wydawnictwo SGGW, Warszawa, pp 17-74.

Wnuk M, Bugno-Poniewierska M, Lewinska A, Oklejewicz B, Zabek T, Bartosz G, Slota E (2011) Age-related changes in genomic stability of horses. Mech Ageing Dev 132: 257-268.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

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