B-cell and T-cell values in peripheral blood in Polish mixed-breed rabbits with addition of blood of meet breeds

Open access

Abstract

In Poland, rabbit is a highly valued animal, due to dietetic and flavour values of its meat, but above all, rabbits tend to be commonly used laboratory animals. The aim of the study was developing standards for counts of B-cells with CD19+ receptor, T-cells with CD5+ receptor, and their subpopulations, namely T-cells with CD4+, CD8+ and CD25+ receptor in the peripheral blood of mixed-breed Polish rabbits with addition of blood of meet breeds, including the assessment of the impact of four seasons of the year and animal sex on the values of the immunological parameters determined. The results showed that the counts of B- and T-cells and their subpopulations in peripheral blood remain within the following ranges: for CD19+ B-cells: 1.05 - 3.05%, for CD5+ T-cells: 34.00 - 43.07%, CD4+ T-cells: 23.52 - 33.23%, CD8+ T-cells: 12.55 - 17.30%, whereas for CD25+ T-cells: 0.72 - 2.81%. As it comes to the season of the year, it was observed that it principally affects the values of CD25+ T-cells, while in the case of rabbit sex, more changes were found in females.

Anon (1987) Information and training materials of the Laboratory Animals Section, General Assembly of the Association of Agriculture Engineers and Technicians, In: Materiały informacyjno-szkoleniowe Sekcji ds. Zwierząt Laboratoryjnych ZG Stowarzyszenia Inżynierów i Techników Rolnictwa, Warszawa, pp 26-77.

Anon (2006) Regulation of the Minister of Agriculture and Rural Development of 10 March 2006 on detailed conditions for maintenance of laboratory animals in experimental units, breeding units and suppliers (Polish Journal of Laws of 2006, No. 50, item 368).

Anon (2013) http://www.nauka.gov.pl/g2/oryginal/2013-05/91344c98ee12f19a3735d49716e9f643.pdf.

Archetti I, Tittarelli C, Ceriolo M, Brivio R, Grilli G, Lavazza A (2008) Serum chemistry and hematology values in commercial rabbits: preliminary data from industrial farms in Northern Italy. Proceedings of 9th World Rabbit Congress, Italy, pp 1147-1151.

Abdel-Azeem AS, Abdel-Azim AM, Darwish AA, Omar EM (2010) Haematological and biochemical observations in four pure breeds of rabbits and their crosses under Egyptian environmental conditions. World Rabbit Sci 18: 103-110.

Black DM, Gilardi KV, Hamilton LP, Williams E, Williams DF, Kelly PA, Gardner I (2009) Hematologic and biochemistry reference values for the endangered riparian brush rabbit (Sylvilagus bachmani riparius). J Wildl Dis 45: 491-496.

Bortolotti A, Castelli D, Bonati E (1989) Hematology and serum chemistry values of adult, pregnant and newborn New Zealand rabbits (Oryctolagus cuniculus). Lab Anim Sci 39: 437-439.

Burnett N, Mathura K, Metivier KS, Holder RB, Brown G, Campell M (2006) An investigation into haematological and serum chemistry parameters of rabbits in Trinidad. World Rabbit Sci 14: 175-187.

Chineke CA, Ologun AG, Ikeobi CO (2006) Haematological parameters in rabbit breeds and crosses in humid tropics. Pak J Biol Sci 9: 2102-2106.

C˛ etin N, Bekyurek T, C˛ etin E (2009) Effects of sex, pregnancy and season on some haematological and biochemical blood values in angora rabbits. Scand J Lab Anim Sci 36: 155-162.

Deptuła W, Górecka-Odkała D, Tokarz-Deptuła B (1995) Dynamics of selected parameters of immunity in rabbits aged from 3 to 5 months. Med Weter 51: 552-554.

Deptuła W, Kostrzewa A, Stosik M, Tokarz-Deptuła B, Wiktorowicz K (1998a) Subpopulations of peripheral blood lymphocytes in rabbits. Nowiny Lekarskie 67: 377-382.

Deptuła W, Niedźwiedzka-Rystwej P, Śliwa J, Tokarz-Deptuła B, Hukowska-Szematowicz B, Pawlikowska M (2009) Specific immunity in healthy rabbits. Centr Eur J Immunol 34: 18-19.

Deptuła W, Tokarz-Deptuła B, Kostrzewa A, Wiktorowicz K, Stosik M (1998b) Cytometric analysis of peripheral blood lymphocyte populations in healthy rabbits. Proceedings of Symposium “Animal husbandry and applied biology”, Wrocław, pp 169-171.

Deptuła W, Tokarz-Deptuła B, Hukowska-Szematowicz B (2004a) Lymphocytes and their subpopulations in peripheral blood of rabbits. Proceedings of VII Conference “Molecular biology in diagnostics of infectious diseases and biotechnology”, Warsaw, Poland, p 55-58.

Deptuła W, Tokarz-Deptuła B, Hukowska B (2004b) Subpopulations of lymphocytes in peripheral blood of healthy 3-to 3,5-month-old rabbits. Proceedings of 13th International Symposium “Molecular and physiological aspects of regulatory processes of the organism”, Kraków, pp 91-92.

Ferrian S, Guerrero I, Blas E, Garcta-Diego FJ, Viana D, Pascual JJ, Corpa JM (2012) How selection for reproduction or foundation for longevity could have affected blood lymphocyte populations of rabbit does under conventional and heat stress conditions. Vet Immunol Immunopathol 150: 53-60.

Fox RR, Baird CW (1970) Diurnal variations in rabbits: hematological parameters. Am J Physiol 218: 1609-1612.

Guerrero I, Ferrian S, Blas E, Pascual JJ, Cano JL, Corpa JM (2011) Evolution of the peripheral blood lymphocyte populations in multiparous rabbit does with two reproductive management rhythms. Vet Immunol Immunopathol 140: 75-81.

Jain JM (1994) Comparative hematologic features of some avian and mammalian species. In: Jain NC (ed), Essentials of veterinary hematology. Lea and Febiger, Philadelphia, pp 367-376.

Jeklova E, Leva L, Faldyna M (2007) Lymphoid organ development in rabbits: major lymphocyte subsets. Dev Comp Immunol 31: 632-644.

Jeklova E, Leva L, Knotigova P, Faldyna M (2009) Age-related changes in selected haematology parameters in rabbits. Res Vet Sci 86: 525-528.

Laird CW, Fox RR, Mitchell BP, Blau EM, Schultz HS (1970) Effect of strain and age on some hematological parameters in the rabbit. Am J Physiol 218: 1613-1617.

Martinec M, Ha¨rtlova´ H, Chodova´ D, Tu°mova´ E, Fucˇikova´ A (2012) Selected haematological and biochemical indicators in different breeds of rabbits. Acta Vet Brno 81: 371-375.

Nowaczyk P, Deptuła W, Tokarz-Deptuła B, Ossowski A, Suproń M (2004) Dynamics of chosen immunological-haematological parameters in annual cycle in rabbits. Centaur Lubuski 61: 6-10.

Nowaczyk P, Deptuła W, Tokarz-Deptuła B (2005) Chosen blood parameters in annual cycle in rabbits. Acta Biol 411: 57-63.

Olayemi FO, Nottidge HO (2007) Effect of age on the blood profiles of the New Zealand rabbit in Nigeria. Afr J Biomed Res 10: 73-76.

O¨ zkan C, Kaya A, Akgu¨l Y (2012) Normal values of haematological and some biochemical parameters in serum and urine of New Zealand white rabbits. World Rabbit Sci 20: 253-259.

Pintor PP, Grassini V (1957) Individual and seasonal spontaneous variations of haematological values in normal male rabbits; statistical survey. Acta Haematol 17: 122-128.

Poljicˇak-Milas N, Kardum-Skelin I, Vudan M, Marenjak TS, Ballarin-Perharić A, Milas Z (2009) Blood cell count analyses and erythrocyte morphometry in New Zealand white rabbits. Vet Arhiv 79: 561-571.

Rohilla PP, Bujaubauah KM, Singh G, Kumar M (2000) Haematological and biochemical studies on pure and crossbred rabbits. Ind J Anim Sci 70: 60-62.

Sabolovic N, Sabolovic D, Guilmin AM (1977) T and B cell surface markers on rabbit lymphocytes. Immunology 32: 581-590.

Tokarz-Deptuła B, Deptuła W (2005) Values of selected immune and haematological parameters in healthy rabbits. Pol J Vet Sci 8: 107-112.

Yaqub LS, Kawu MU, Ayo JO (2013) Influence of reproductive cycle, sex, age and season on haematologic parameters in domestic animals: a review. J Cell Anim Biol 7: 37-43.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

Journal Information


IMPACT FACTOR 2016: 0.697
5-year IMPACT FACTOR: 0.773

CiteScore 2016: 0.73

SCImago Journal Rank (SJR) 2016: 0.315
Source Normalized Impact per Paper (SNIP) 2016: 0.486

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 229 227 16
PDF Downloads 64 64 3