Distribution of classical enterotoxin genes in staphylococci from milk of cows withand without mastitis and the cowshed environment

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The aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with- and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulase-negative staphylococci (CNS) isolates, among them in 9 (11.4%) isolates of S. xylosus, 5 (16.7%) S. sciuri, 3 (10.3%) S. epidermidis and in 5 (22.7%) Staphylococcus spp. In some CNS 2 or 3 genes were detected simultaneously. Among the investigated enterotoxin genes, sec was the most prevalent (70%). The genes encoding enterotoxin B and D were detected in 5 (16.7%) and 6 (20%) isolates, respectively. The lowest number of isolates had sea and see genes.

The genes encoding enterotoxins were often identified in staphylococci from milk of cows with mastitis (73.4% of detected genes), while only 6 (20%) isolates from milk of cows without mastitis and 2 (6.6%) isolates from cowshed environment were positive for enterotoxin genes.

The results showed that CNS from bovine milk, like S. aureus, carried enterotoxin genes and may pose a risk for public health.

Asao T, Kumeda Y, Kawai T, Shibata T, Oda H, Haruki K, Nakazawa H, Kozaki S (2003) An extensive outbreak of staphylococcal food poisoning due to low-fat milk in Japan: estimation of enterotoxin A in the incriminated milk and powdered skim milk. Epidemiol Infect 130: 33-40.

Baker MD, Acharya KR (2004) Superantigens: structurefunction relationships. Int J Med Microbiol 293: 529-537.

Bania J, Dąbrowska A, Bystroń J, Korzekwa K, Chrzanowska J, Molenda J (2006) Distribution of newly described enterotoxin-like genes in Staphylococcus aureus from food. Int J Food Microbiol 108: 36-41.

Becker K, Roth R, Peters G (1998) Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene. J Clin Microbiol 36: 2548-2553.

Blaiotta G, Fusco V, von Eiff C, Villani F, Becker K (2006) Biotyping of enterotoxigenic Staphylococcus aureus by enterotoxin gene cluster (egc) polymorphism and spa typing analyses. Appl Environ Microbiol 72: 6117-6123.

Boynukara B, Gulhan T, Alisarli M, Gurturk K, Solmaz H (2008) Classical enterotoxigenic characteristics of Staphylococcus aureus strains isolated from bovine subclinical mastitis in Van, Turkey. Int J Food Microbiol 125: 209-211.

Collery MM, Smyth DS, Twohig JM, Shore AC, Coleman DC, Smyth CJ (2008) Molecular typing of nasal carriage isolates of Staphylococcus aureus from an Irish university student population based on toxin gene PCR, agr locus types and multiple locus, variable number tandem repeat analysis. J Med Microbiol 57: 348-358.

Cunha ML, Calsolari RA (2008) Toxigenicity in Staphylococcus aureus and coagulase-negative staphylococci: epidemiological and molecular aspects. Microbiol Insights 1: 13-24.

De Freitas Guimara˜es F, Nóbrega DB, Richini-Pereira VB, Marson PM, Figueiredo Pantoja JC, Langoni H (2013) Enterotoxin genes in coagulase-negative and coagulase- positive staphylococci isolated from bovine milk. J Dairy Sci 96: 2866-2872.

Dinges MM, Orwin PM, Schlievert PM (2000) Exotoxins of Staphylococcus aureus. Clin Microbiol Rev 13: 16-34.

Herman A, Kappler JW, Marrack P, Pullen AM (1991) Superantigens: mechanism of T-cell stimulation and role in immune responses. Annu Rev Immunol 9: 745-772.

Jørgensen HJ, Mørk T, Høga°sen HR, Rørvik LM (2005) Enterotoxigenic Staphylococcus aureus in bulk milk in Norway. J Appl Microbiol 99: 158-166.

Kot B, Piechota M, Antos-Bielska M, Zdunek E, Wolska KM, Binek T, Olszewska J, Guliński P, Trafny EA (2012a) Antimicrobial resistance and genotypes of staphylococci from bovine milk and the cowshed environment. Pol J Vet Sci 15: 741-749.

Kot B, Piechota M, Wolska KM, Frankowska A, Zdunek E, Binek T, Kłopotowska E, Antosiewicz M (2012b) Phenotypic and genotypic antimicrobial resistance of staphylococci from bovine milk. Pol J Vet Sci 15: 677-683.

Lina G, Bohach GA, Nair SP, Hiramatsu K, Jouvin-Marche E, Mariuzza R (2004) Standard nomenclature for the superantigens expressed by Staphylococcus. J Infect Dis 189: 2334-2336.

Njage PM, Dolci S, Jans C, Wangoh J, Lacroix C, Meile L (2013) Biodiversity and enterotoxigenic potential of staphylococci isolated from raw and spontaneously fermented camel milk. Br Microbiol Res J 3: 128-138.

Orwin PM, Leung DY, Donahue HL, Novick RP, Schliewert PM (2001) Biochemical and biological properties of staphylococcal enterotoxin K. Infect Immun 69: 360-366.

Park JY, Fox LK, Seo KS, McGuire MA, Park YH, Rurangirwa FR, Sischo WM, Bohach GA (2011) Detection of classical and newly described staphylococcal superantigen genes in coagulase-negative staphylococci isolated from bovine intramammary infections. Vet Microbiol 147: 149-154.

Podkowik M, Park JY, Seo KS, Bystroń J, Bania J (2013) Enterotoxigenic potential of coagulase-negative staphylococci. Int J Food Microbiol 163: 34-40.

Sampimon OC, Barkema HW, Berends IM, Sol J, Lam TJ (2009) Prevalence and herd-level risk factors for intramammary infection with coagulase-negative staphylococci in Dutch dairy herds. Vet Microbiol 134: 37-44.

Scherrer D, Corti S, Muehlherr JE, Zweifel C, Stephan R (2004) Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep. Vet Microbiol 101: 101-107.

Weese JS (2012) Staphylococcal control in the veterinary hospital. Vet Dermatol 23: 292-298.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

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