In vivo inhibitory effect of Aloe vera gel on the ability of mouse parental splenic lymphocytes to induce cutaneous angiogenesis in recipient F1 mice

Open access


Lymphocyte-induced angiogenesis test (LIA) is a model of local graft-versus-host (GVH) reaction, marker of the earliest events resulting from activation of donor lymphocytes after contact with host semi-allogeneic histocompatibility antigens. The effect of in vivo oral administration of Aloe vera gel for 21 days to maternal strain (Balb/c) donor mice on the ability of their splenic lymphocytes to induce cutaneous angiogenesis (LIA test) in F1 Balb/c x C3H recipients, was studied.

Results: Neovascular reaction evaluated 72 hours after cells grafting was significantly lower in F1 mice grafted with lymphocytes collected from Aloe- fed donors, than in recipients of lymphocytes collected from respective controls. Conclusions: This observation opens the promise of safe and ethically acceptable possibility of use of Aloe vera gel in human donors in prevention of GVHD in recipients of bone marrow grafts.

Ambrus CM, Ambrus JL, Feltz ET (1966) Mitigation of secondary disease in mice receiving bone marrow from donors pretreated with cortisone and alkylating agents. Transplantation 4: 245-249.

Auerbach R, Kubai L, Sidky Y (1976) Angiogenesis induction by tumors, embryonic tissues and lymphocytes. Cancer Res 36: 3435-3440.

Bałan BJ, Stankiewicz W, Skopinska-Różewska E, Zdanowski R, Kocik J, Lewicki S, Sokolnicka I, Nowakowska J, Rogala E, Skopiński P (2013) The effect of multi-component herbal remedy PERVIVO on cellular immunity and tumor angiogenesis in mice. Centr Eur J Immunol 38: 54-61.

Barrantes E, Guinea M (2003) Inhibition of collagenase and metalloproteinases by aloins and aloe gel. Life Sci 72: 843-850.

Bernhardt WM, Gottman U, Doyon F, Buchholz B, Campean V, Schodel J, Reisenbuechler A Klaus S, Arend M, Flippin L, Willam C, Wiesener MS, Yard B, Warnecke C, Eckardt KU (2009) Donor treatment with a PHD-inhibitor activating HIFs prevents graft injury and prolongs survival in an allogenic kidney transplant model.

Proc Natl Acad Sci USA 106: 21276-21281.

Cooper MH, Markus PM, Cai X, Starzl TE, Fung JJ (1991) Prolonged prevention of acute graft-versus-host disease after allogeneic bone marrow transplantation by donor pretreatment using FK506. Transplant Proc 23: 3238-3239.

Eamlamnam K, Patumraj S, Visedopas N, Thong-Ngam D (2006) Effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing in rats. World J Gastroenterol 12: 2034-2039.

Habeeb F, Stables G, Bradbury F, Nong S, Cameron P, Plevin R, Ferro VA (2007) The inner gel component of Aloe vera suppresses bacterial- induced pro-inflammatory cytokines from human immune cells. Methods 42: 388-393.

Jacobs P (1985) Cyclosporin A pretreatment of both donor and recipient undergoing allogeneic bone marrow transplantation. Scand J Haematol 35: 386-393.

Krenger W, Snyder KM, Byon JC, Falzarano G, Ferrara JL (1995) Polarized type 2 alloreactive CD4+ and CD8+ donor T cells fail to induce experimental acute graft-versus- host disease. J Immunol 155: 585-593.

Mac Donald KP, Rowe W, Filippich C, Thomas R, Clouston AD, Welply JK, Hart DN, Ferrara JL, Hill GR (2003) Donor pretreatment with progenipoietin -1 is superior to granulocyte colony- stimulating factor in preventing gvh disease after allogeneic stem cell transplantation. Blood 101: 2033-2042.

Pan L, Delmonte J Jr, Jalonen CK, Ferrara JL (1995) Pretreatment of donor mice with granulocyte colony-stimulating factor polarizes donor T lymphocytes toward type-2 cytokine production and reduces severity of experimental graft-versus-host disease. Blood 86: 4422-4429.

Prabjone R, Thong-Ngam D, Wisedopas N, Chatsuwan T, Patumraj S (2006) Anti-inflammatory effects of Aloe vera on leukocyte-endothelium interaction in the gastric microcirculation of Helicobacter pylori-infected rats. Clin Hemorheol Microcirc 35: 359-366.

Rotta M, Storer BE, Storb RF, Martin PJ, Heimfeld S, Peffer A, Maloney DG, Deeg HJ, Sandmaier BM, Appelbaum FR, Mielcarek M (2010) Donor statin treatment protects against severe acute graft-versus-host disease after related allogeneic hematopoietic cell transplantation. Blood 115(6):1288-1295.

Rus V, Svetic A, Nguyen P, Gause WC, Via CS (1995) Kinetics of Th1 and Th2 cytokine production during the early course of acute and chronic murine graft-versus-host dis- ease. Regulatory role of donor CD8+ T cells. J Immunol 155: 2396-2406.

Sehgal I, Winters WD, Scott M, Kousoulas K (2013) An in vitro and in vivo toxicologic evaluation of a stabilized Aloe vera gel supplement drink in mice. Food Chem Toxicol 55: 363-370.

Sidky YA, Auerbach R (1975) Lymphocyte-induced angiogenesis: a quantitative and sensitive assay of the graft-versus-host reaction. J Exp Med 141: 1084-1100.

Siwicki AK, Skopińska-Różewska E, Hartwich M, Wójcik R, Bakuła T, Furmanowa M, Bałan BJ, Sommer E, Mielcarek S, Buchwald W, Krajewska-Patan A, Mścisz A, Mrozikiewicz PM, Bany J (2007) The influence of Rhodiola rosea extracts on non-specific and specific cellular immunity in pigs, rats and mice. Centr Eur J Immunol 32: 84-91.

Skopińska-Różewska E, Sommer E, Sokolnicka I, Bany J, Guzewska J, Furmanowa M (2002) The effect of Centella asiatica, Echinacea purpurea and Melaleuca alternifolia on cellular immunity in mice. Centr Eur J Immunol 27: 142-148.

Skopińska-Różewska E, Wasiutyński A, Skopiński P, Siwicka D, Zdanowski R, Bodera P (2011a) In vivo effect of two complex herbal remedies Echinasal and Bioaron C on antibody production and immunological angiogenesis in mice. Centr Eur J Immunol 36: 139-144.

Skopinska-Różewska E, Wasiutyński A, Sommer E, Skopiński P, Pastewka K, Zdanowski R, Bany J (2011b) Modulatory effect of Echinacea pallida on cellular immunity and angiogenesis in mice. Centr Eur J Immunol 36: 18-23.

Skopińska-Różewska E, Wójcik R, Siwicki AK, Sommer E, Wasiutyński A, Furmanowa M, Malinowski M, Mazurkiewicz M (2008) The effect of Rhodiola quadrifida extracts on cellular immunity in mice and rats. Pol J Vet Sci 11: 105-111.

Skopiński P, Sommer E, Skopińska-Różewska E (2001) The effect of coastal salt lake mud distillate mixed with cinnamic acid and coumarin (FIBS) on immunological and inflammatory angiogenesis. Terapia IX3: 14-15.

Talmadge J, Chavez J, Jacobs L, Munger C, Chinnah T, Chow JT, Williamson D, Yates K (2004) Fractionation of Aloe vera L. inner gel , purification and molecular profiling of activity. Int Immunopharmacol 4: 1757-1773.

Tawara I, Sun Y, Liu C, Toubai T, Nieves E, Evers R, Alrubaie M, Mathewson N, Tamaki H, Reddy P (2012) Donor-but not host - derived interleukin-10 contributes to the regulation of experimental graft-versus-host disease. J Leukoc Biol 91: 667-675.

Vijayalakshmi D, Dhandapani R, Jayaveni S, Jithendra PS, Rose C, Mandal AB (2012) In vitro anti-inflammatory activity of Aloe vera by down regulation of MMP-9 in peripheral blood mononuclear cells. J Ethnopharmacol 141: 542-546.

Volk EE, Domen RE, Smith ML (1999) An examination of ethical issues raised in the pretreatment of normal volunteer granulocyte donors with granulocyte colony-stimulating factor. Arch Pathol Lab Med 123: 508-513.

Wójcik R, Siwicki AK, Skopińska-Rożewska E, Wasiutyński A, Sommer E, Furmanowa M (2009) The effect of Chinese medicinal herb Rhodiola kirilowii extracts on cellular immunity in mice and rats. Pol J Vet Sci 12: 399-405.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

Journal Information

IMPACT FACTOR 2016: 0.697
5-year IMPACT FACTOR: 0.773

CiteScore 2016: 0.73

SCImago Journal Rank (SJR) 2016: 0.315
Source Normalized Impact per Paper (SNIP) 2016: 0.486


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 192 157 15
PDF Downloads 137 131 4