Complete mitochondrial genome of wild aurochs (Bos primigenius) reconstructed from ancient DNA

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Extinct aurochs (Bos primigenius), accepted as the ancestor of domestic cattle, was one of the largest wild animals inhabiting Europe, Asia and North Africa. The gradual process of aurochs extinction finished in Poland in 1627, were the last recorded aurochs, a female, died. Some aspects of cattle domestication history and the distribution of aurochs genetic material among modern cattle breeds still remain unclear. Analyses of ancient DNA (aDNA) from bone sample deliver new genetic information about extinct wild aurochs as well as modern cattle phylogeny. DNA was extracted from a fragment of aurochs fossil bone found in the Pisz Forest, Poland. The sample was radiocarbon- dated to about 1500 yBP. The aDNA was used for Whole Genome Amplification in order to form a DNA bank. Auroch mitochondrial DNA sequences were amplified using sets of 41 primers overlapping the whole mtDNA, cloned and sequenced. The sequence of the whole mitochondrial genome was reconstructed and deposed in GenBank [GenBank:JQ437479]. Based on the phylogenetic analyses of the Bovine mitochondrial genomes, a phylogenetic tree was created. As expected, the tree clearly shows that the mtDNA sequence of the analyzed PWA (Polish Wild Aurochs) individual belongs to haplogroup P. In the course of the comparative mtDNA analysis we identified 30 nucleotide marker positions for haplogroup P and nine unique PWA differences compared to the two remaining haplotype P representatives. Our analysis provides the next step to the reconstruction of the demographic history of this extinct but still exciting species.

Achilli A, Bonfiglio S, Olivieri A, Malusg A, Pala M, Hooshiar Kashani B, Perego UA, Ajmone-Marsan P, Liotta L, Semino O, Bandelt HJ, Ferretti L, Torroni A (2009) The multifaceted origin of taurine cattle reflected by the mitochondrial genome. PLoS One 4: e5753.

Achilli A, Olivieri A, Pellecchia M, Uboldi C, Colli L, Al-Zahery N, Accetturo M, Pala M, Hooshiar Kashani B, Perego UA, Battaglia V, Fornarino S, Kalamati J, Houshmand M, Negrini R, Semino O, Richards M, Macaulay V, Ferretti L, Bandelt HJ, Ajmone-Marsan P, Torroni A (2008) Mitochondrial genomes of extinct aurochs survive in domestic cattle. Curr Biol 18: R157-R158.

Anderson S, de Bruijn MH, Coulson AR, Eperon IC, Sanger F, Young IG (1982) Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome. J Mol Biol 156: 683-717.

Beja-Pereira A, Caramelli D, Lalueza-Fox C, Vernesi C, Ferrand N, Casoli A, Goyache F, Royo LJ, Conti S, Lari M, Martini A, Ouragh L, Magid A, Atash A, Zsolnai A, Boscato P, Triantaphylidis C, Ploumi K, Sineo L, Mallegni F, Taberlet P, Erhardt G, Sampietro L, Bertranpetit J, Barbujani G, Luikart G, Bertorelle G (2006) The origin of European cattle: evidence from modern and ancient DNA. Proc Natl Acad Sci U S A 103: 8113-8118.

Bonfiglio S, Achilli A, Olivieri A, Negrini R, Colli L, Liotta L, Ajmone-Marsan P, Torroni A, Ferretti L (2010) The enigmatic origin of bovine mtDNA haplogroup R: sporadic interbreeding or an independent event of Bosprimigenius domestication in Italy? PLoS One 5: e15760.

Brown MD, Voljavec AS, Lott M T, Torroni A, Yang CC, Wallace DC (1992) Mitochondrial DNA complex I and III mutations associated with Leber’s hereditary optic neuropathy. Genetics 130: 163-173.

Bruford MW, Bradley DG, Luikart G (2003) DNA markers reveal the complexity of livestock domestication. Nat Rev Genet 4: 900-910.

Chen S, Lin BZ, Baig M, Mitra B, Lopes RJ, Santos AM, Magee DA, Azevedo M, Tarroso P, Sasazaki S, Ostrowski S, Mahgoub O, Chaudhuri TK, Zhang YP, Costa V, Royo LJ, Goyache F, Luikart G, Boivin N, Fuller DQ, Mannen H, Bradley DG, Beja-Pereira A (2010) Zebu cattle are an exclusive legacy of the South Asia neolithic. Mol Biol Evol 27: 1-6.

Drummond AJ, Ho SYW, Phillips MJ, Rambaut A (2006) Relaxed phylogenetics and dating with confidence. PLoS Biol 4:e88.

Drummond AJ, Rambaut A (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 7: 214.

Edwards CJ, Bollongino R, Scheu A, Chamberlain A, Tresset A, Vigne JD, Baird JF, Larson G, Ho SY, Heupink TH, Shapiro B, Freeman AR, Thomas MG, Arbogast RM, Arndt B, Bartosiewicz L, Benecke N, Budja M, Chaix L, Choyke AM, Coqueugniot E, Döhle HJ, Göldner H, Hartz S, Helmer D, Herzig B, Hongo H, Mashkour M, Ozdogan M, Pucher E, Roth G, Schade-Lindig S, Schmölcke U, Schulting RJ, Stephan E, Uerpmann HP, Vörös I, Voytek B, Bradley DG, Burger J (2007) Mitochondrial DNA analysis shows a Near Eastern Neolithic origin for domestic cattle and no indication of domestication of European aurochs. Proc Biol Sci 274: 1377-1385.

Edwards CJ, Magee DA, Park SD, McGettigan PA, Lohan AJ, Murphy A, Finlay EK, Shapiro B, Chamberlain AT, Richards MB, Bradley DG, Loftus BJ, MacHugh DE (2010) A complete mitochondrial genome sequence from a mesolithic wild aurochs (Bos primigenius). PLoS One 5: e9255.

Lari M, Rizzi E, Mona S, Corti G, Catalano G, Chen K, Vernesi C, Larson G, Boscato P, De Bellis G, Cooper A, Caramelli D, Bertorelle G (2011) The complete mitochondrial genome of an 11,450-year-old aurochsen (Bos primigenius) from Central Italy. BMC Evol Biol 11: 32.

Mona S, Catalano G, Lari M, Larson G, Boscato P, Casoli A, Sineo L, Di Patti C, Pecchioli E, Caramelli D, Bertorelle G (2010) Population dynamic of the extinct European aurochs: genetic evidence of a north-south differentiation pattern and no evidence of post-glacial expansion. BMC Evol Biol 10: 83.

Rohland N, Hofreiter M (2007) Ancient DNA extraction from bones and teeth. Nat Protoc 2: 1756-1762.

Schmitt T (2007) Molecular biogeography of Europe: Pleistocene cycles and postglacial trends. Front Zool 4: 11.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731-2739.

Troy CS, MacHugh DE, Bailey JF, Magee DA, Loftus RT, Cunningham P, Chamberlain AT, Sykes BC, Bradley DG (2001) Genetic evidence for Near-Eastern origins of European cattle. Nature 410: 1088-1091.

Willerslev E, Cooper A (2005) Ancient DNA. Proc Biol Sci 272: 3-16.

Zeyland J, Wolko L, Lipiński D, Woźniak A, Nowak A, Szalata M, Bocianowski J, Słomski R (2012) Tracking of wisent-bison-yak mitochondrial evolution. J Appl Genet 53: 317-322.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

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