1 Institute of Veterinary, Lithuanian University of Health Sciences, Tilzes g. 18, LT-47181, Kaunas, Lithuania
2 Laboratory of Antimicrobial and Biocide Resistance, Interdisciplinary Centre of Research in Animal Health, Faculty of Veterinary Medicine, Technical University of Lisbon, Av. da Universidade Tecnica, 1300-477 Lisboa, Portugal
During 2011, 160 nasal samples were taken from pigs on 8 different farms in Lithuania. Four methicillin-resistant Staphylococcus aureus (MRSA) isolates were obtained. The isolates were ST398, spa type t011 and SCCmec V and none carried the lukF/lukS genes. Strains were resistant to tetracycline, attributed to tetK and tetM genes, and to erythromycin owing to the ermB gene. One MRSA strain was resistant to trimethoprim/sulfamethoxazole and carried the dfrK gene. This is the first report on the presence and characteristics of livestock-associated MRSA isolated from pigs in Lithuania.
European Food Safety Authority (2009) Analysis of the baseline survey on the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in holdings with breeding pigs, in the EU, 2008. Part A: MRSA prevalence estimates. EFSA Journal 7: 1376.
Marszalek M, Empel J, Zmudzki J, Nowak A, Pejsak Z, Hryniewicz W (2009) First methicillin-resistant Staphylococcus aureus of ST398 isolated from Poland from nasal carriers. 19th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID). 16.05.2009 - 19.05.2009.
Pomba C, Baptista FM, Couto N, Louc¸a˜o F, Hasman H (2010) Methicillin-resistant Staphylococcus aureus CC398 isolates with indistinguishable ApaI restriction patterns in colonized and infected pigs and humans. J Antimicrob Chemother 65: 2479-2484.
van Wamel WJ, Hansenova Manaskova S, Fluit AC, Verbrugh H, de Neeling AJ, van Duijkeren E, van Belkum A (2010) Short term micro-evolution and PCR-detection of methicillin-resistant and - susceptible Staphylococcusaureus sequence type 398. Eur J Clin Microbiol Infect Dis 29: 119-122.