Lymphoma is one of the most common malignant tumours occurring in dogs. Since there is a constant need for new, more comprehensive laboratory diagnostic tools which permit the precise determination of many tumour-related factors we decided to verify whether the use of microarray analysis could be helpful in classifying lymphomas. The study was performed on samples collected from 7 dogs in which multicentric lymphoma was recognized. Among this group we were able to identify one sub-cluster of transcriptionally similar tumours, which completely differed in terms of the histopathological examination. Among them there were one diffuse large B cell lymphoma, one diffuse macronucleolated medium-sized cell lymphoma and one pleomorphic mixed small and large T-cell lymphoma. The lymphomas belonging to the sub-cluster differed from other analysed tumours in the expression of more than 100 genes of which only 18 were described earlier in regard to lymphomas.
Dobson JM, Blackwood LB, McInnes EF, Bostock DE, Nicholls P, Hoather TM, Tom BD (2001) Prognostic variables in canine multicentric lymphosarcoma. J Small Anim Pract 42: 377-384.
Foulner-Fleury C, Magnol JP, Bricaire P, Marchal T, Chabanne L, Delverdier A, Bryon PA, Felman P (1997) Cytohistological and immunological classification of canine malignant lymphomas: comparison with human non-Hodgkin’s lymphomas. J Comp Pathol 117: 35-59.
Foulner-Fleury C, Ponce F, Felman P, Blavier A, Bonnefont C, Chabanne L, Marchal T, Cadore JL, Goy-Thollot I, Ledieu D, Ghernati I, Magnol JP (2002) Canine T-cell lymphomas: a morphological, immunological, and clinical study of 46 new cases. Vet Pathol 39: 92-109.
Guija de Arespacochaga A, Schwendenwein I, Weissenbock H (2007) Retrospective study of 82 cases of canine lymphoma in Austria based on the Working Formulation and immunophenotyping. J Comp Pathol 136: 186-192.
Iqbal J, d’Amore F, Hu Q, Chan WC, Fu K (2006) Gene arrays in lymphoma: where will they fit in? Curr Hematol Malig Rep 1: 129-136.
Iqbal J, Weisenburger DD, Greiner TC, Vose JM, McKeithan T, Kucuk C, Geng H, Deffenbacher K, Smith L, Dybkaer K, Nakamura S, Seto M, Delabie J, Berger F, Loong F, Au WY, Ko YH, Sng I, Armitage JO, Chan WC (2010) Molecular signatures to improve diagnosis in peripheral T-cell lymphoma and prognostication in angioimmunoblastic T-cell lymphoma. Blood 115: 1026- -1036 Jagielski D, Lechowski R, Hoffman-Jagielska M, Winiarczyk S (2002) A retrospective study of the incidence and prognostic factors of multicentric lymphoma in dogs (1998-2000). J Vet Med A 49: 419-424.
Ledieu D, Mathias V, Bernaud J, Rigal D (2005) Use of human cDNA arrays to analyze canine gene expression in two cases of canine lymphoma. Comp Clin Pathol 14: 125-129
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-delta method. Methods 25: 402-408.
Marconato L (2010) The staging and treatment of multicentric high-grade lymphoma in dogs: a review of recent developments and future prospects. Vet J 188: 34-38 Marconato L, Stefanello D, Valenti P, Bonfanti U, Comazzi R, Roccabianca C, Caniatti M, Romanelli G, Massari F, Zini E (2011) Predictors of long-term survivals in dogs with high-grade multicentric lymphoma. J Am Vet Med Assoc 238: 480-485.
Pastor M, Chalvet-Monfray K, Marchal T, Keck G, Magnol JP, Foulner-Fleury C, Ponce F (2009) Genetic and environmental risk indicators in canine non-Hodgkin’s lymphomas: breed associations and geographic distribution of 608 cases throughout France over 1 year. J Vet Intern Med 23: 301-310.
Ponce F, Magnol JP, Ledieu D, Marchal T, Turinelli V, Chalvet-Monfray K, Foulner-Fleury C (2004) Prognostic significance of morphological subtypes in canine malignant lymphomas during chemotherapy. Vet J 167: 158- -166.
Sapierzyński R (2010) Practical aspects of immunocytochemistry in canine lymphomas. Pol J Vet Sci 13: 661-668.
Sapierzyński R, Micuń J (2009) Lymphadenomegaly in dogs - cytological study. Pol J Vet Sci 12: 263-268.
Sapierzyński R, Micuń J, Jagielski D, Jurka P (2010) Cytopathology of canine lymphomas (100 cases). Pol J Vet Sci 13: 653-659.
Smyth GK (2004) Linear models and empirical Bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol 3 article 3 DOI: 10.2202/1544-6115.1027 Starkey MP, Murphy S (2009) Using lymph node fine needle aspirates for gene expression profiling of canine lymphoma. Vet Comp Oncol 8: 56-71 Teske E (1994) Canine malignant lymphoma: a review and comparison with human non-Hodkin’s lymphoma. Vet Q 16:209-219 Teske E, van Heerde P (1996) Diagnostic value and reproducibility of fine-needle aspiration cytology in canine malignant lymphoma. Vet Q 18: 112-115.
Teske E, van Heerde P, Rutteman GR, Kurzman ID, Moore PF, MacEwen EG (1994) Prognostic factors for treatment of malignant lymphoma in dogs. J Am Vet Med Assoc 205: 1722-1728.
Valli VE, San Myint M, Barthel A, Bienzle D, Caswell J, Colbatzky F, Durham A, Ehrhart EJ, Johnson Y, Jones C, Kiupel M, Labelle P, Lester S, Miller (2011) Classification of canine malignant lymphomas according to the World Health Organization Criteria. Vet Pathol 48: 198- -211.