Proliferation activity in canine lymphomas

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Forty five lymphomas, 14 of T cell origin, 28 of B cell and 3 with null-cell phenotype, were included in this study. Tumors were classified according to the updated Kiel classification system adapted to canine lymphomas. The percentage of Ki67+ cells and mitotic index (MI) were estimated in each specimen. Most of lymphomas (39 of 45) had high proliferation activity. Among them in 27 cases 50-70% of lymphoma cells expressed Ki67, the highest Ki67 expression (>70% Ki67+ cells) was identified less frequently, in 12 cases. Moderate Ki67 expression (20-50% positive cells) was observed in 5 cases, only one tumor had low Ki67 expression (<20% positive cells). Lower percentage of Ki67+ cells was usually accompanied with lower MI. The mean MI values in discussed groups differed significantly. Mean MI value was also significantly higher in T cell than in B cell lymphomas (4.30 vs. 3.33). Moreover, high positive correlation between the expression of Ki67 and MI was found (r = 0.668; P ≤ 0.001). In T-cell tumors the correlation was very high (r = 0.83; P ≤ 0.001) and in B-cell lymphomas the correlation was high (r = 0.61; P ≤ 0.001). There were also differences between mean MI values in the lymphomas of different morphological subtypes, but in some of them high variations in the range of MI values were identified and wide overlaps of MI between individual cases from different subtypes were observed. Because of differences in the proliferation activity in single cases of the same subtype of lymphoma, the proliferation activity assessment may be helpful to chose appropriate scheme of treatment and should be commonly performed during routine histopathological diagnosis of canine lymphomas.

Braylan RC (1993) Flow-cytometric DNA analysis in the diagnosis and prognosis of lymphoma. Am J Clin Pathol 99: 374-380.

Brunning RD, Borowitz M, Matuates E, Head D, Flandrin G, Swerdlow SH, Bennett JM (2001) Precursor B-cell and T-cell neoplasms. In: Jaffe ES, Harris NL, Stein H, Vardiman JM (eds) World Health Organization Classification of Tumors. Pathology and Genetics. Tumors of Haematopoietic and Lymphoid Tissues. IARC Press Lyon, 1st ed., pp 111-117.

Carter RF, Valli VE, Lumsden JH (1986) The cytology, histology and prevalence of cell types in canine lymphoma classified according to the National Cancer Institute Working Formulation. Can J Vet Res 50: 154-164.

Diebold J, Jaffe ES, Raphael M, Warnke RA (2001) Burkitt lymphoma. In: Jaffe ES, Harris NL, Stein H, Vardiman JM (eds) World Health Organization Classification of Tumors. Pathology and Genetics. Tumors of Haematopoietic and Lymphoid Tissues. IARC Press Lyon, 1st ed., pp 181-184.

Dzimira S (2007) Immunocytochemical and cytomorphometric diagnostics of malignant lymphomas in dogs. Bull Vet Inst Pulawy 51: 71-78.

Fournel-Fleury C, Magnol JP, Bricaire P, Marchal T, Chabanne L, Delverdier A, Bryon PA, Felman P (1997a) Cytohistological and immunological classification of canine malignant lymphomas: comparison with human non-Hodgkin’s lymphomas. J Comp Pathol 117: 35-59.

Fournel-Fleury C, Magnol JP, Chabanne L, Ghernati I, Marchal T, Bonnefond C, Bryon PA, Felman P (1997b) Growth fractions in canine non-Hodgkin’s lymphomas as determined in situ by the expression of the Ki-67 antigen. J Comp Pathol 117: 61-72.

Fournel-Fleury C, Ponce F, Felman P, Blavier A, Bonnefont C, Chabanne L, Marchal T, Cadore JL, Goy-Thollot I, Ledieu D, Ghernati I, Magnol JP (2002) Canine T-cell lymphomas: a morphological, immunological, and clinical study of 46 new cases. Vet Pathol 39: 92-109.

Grierson HL, Wooldridge TN, Purtilo DT, Pierson J, Bast M, Wooldridge L, Armitage JO, Weisenburger DD (1990) Low proliferative activity is associated with a favorable prognosis in peripheral T-cell lymphoma. Cancer Res 50: 4845-4848.

Grogan TM, Lippman SM, Spier CM, Slymen DJ, Rybski JA, Rangel CS, Richter LC, Miller TP (1988) Independent prognostic significance of a nuclear proliferation antigen in diffuse large cell lymphomas as determined by the monoclonal antibody Ki-67. Blood 71: 1157-1160.

Hall PA, Richards MA, Gregory WM, d’Ardenne AJ, Lister TA, Stansfeld AG (1988) The prognostic value of Ki67 immunostaining in non-Hodgkin’s lymphoma. J Pathol 154: 223-235.

Joensuu H, Klemi PJ, Eerola E (1988) Diagnostic value of DNA flow cytometry combined with fine needle aspiration biopsy in lymphomas. J Pathol 154: 237-245.

Katz RL, Wojcik EM, el-Naggar AK, Ordonez NG, Johnston DA (1993) Proliferation markers in non-Hodgkin’s lymphoma. A comparative study between cytophotometric quantitation of Ki-67 and flow cytometric proliferation index on fine needle aspirates. Anal Quant Cytol Histol 15: 179-186.

Krygier-Stojalowska A, Urasinski I, Kuchnio M, Gapski Z (1993) DNA-ploidy in non Hodgkin’s lymphomas of lower malignancy. Acta Haematol Pol 24: 153-160.

Macartney JC, Camplejohn RS, Morris R, Hollowood K, Clarke D, Timothy A (1991) DNA flow cytometry of follicular non-Hodgkin’s lymphoma. J Clin Pathol 44: 215-218.

Mioduszewska O (1998): Patologia chłoniaków i ziarnicy złośliwej. Pol J Pathol 49 Ssuppl): 26-27, 59-60.

Phillips BS, Kass PH, Naydan DK, Winthrop MD, Griffey SM, Madewell BR (2000) Apoptotic and proliferation indexes in canine lymphoma. J Vet Diagn Invest 12: 111-117.

Ponce F, Magnol JP, Marchal T, Chabanne L, Ledieu D, Bonnefont C, Felman P, Fournel-Fleury C (2003) High-grade canine T-cell lymphoma/leukemia with plasmacytoid morphology: a clinical pathological study of nine cases. J Vet Diagn Invest 15: 330-337.

Ponce F, Marchal T, Magnol JP, Turinelli V, Ledieu D, Bonnefont C, Pastor M, Delignette ML, Fournel-Fleury C (2010) A morphological study of 608 cases of canine malignant lymphoma in France with a focus on comparative similarities between canine and human lymphoma morphology. Vet Pathol 47: 414-433.

Riondato F (2011) Canine lymphoma: going with the flow. Vet J 188: 134-135.

Schwartz BR, Pinkus G, Bacus S, Toder M, Weinberg DS (1989) Cell proliferation in non-Hodgkin’s lymphomas. Digital image analysis of Ki-67 antibody staining. Am J Pathol 134: 327-336.

Teske E, Rutteman GR, Kuipers-Dijkshoorn NJ, van Dierendonck JH, van Heerde P, Cornelisse CJ (1994) DNA ploidy and cell kinetic characteristics in canine non-Hodgkin’s lymphoma. Exp Hematol 21: 579-584.

Valli VE, San Myint M, Barthel A, Bienzle D, Caswell J, Colbatzky F, Durham A, Ehrhart EJ, Johnson Y, Jones C, Kiupel M, Labelle P, Lester S, Miller M, Moore P, Moroff S, Roccabianca P, Ramos-Vara J, Ross A, Scase T, Tvedten H, Vernau W (2011) Classification of canine malignant lymphomas according to the World Health Organization criteria. Vet Pathol 48: 198-211.

Weiss LM, Strickler JG, Medeiros LJ, Gerdes J, Stein H, Warnke RA (1987) Proliferative rates of non-Hodgkin’s lymphomas as assessed by Ki-67 antibody. Hum Pathol 18: 1155-1159.

Winter JN, Andersen J, Variakojis D, Gordon LI, Fisher RI, Oken MM, Neiman RS, Jiang S, Bauer KD (1996) Prognostic implications of ploidy and proliferative activity in the diffuse aggressive non-Hodgkin’s lymphomas. Blood 88: 3919-3925.

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

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