Classical swine fever (CSF) is a notifiable, highly contagious disease of swine controlled mainly with costly administrative methods. Swine may be infected not only with classical swine fever virus (CSFV), but also with other, non porcine, genetically and antigenically related pestiviruses. Differentiation of infections with CSFV and other pestiviruses is a crucial element of diagnostics.
In the present study two real-time PCR methods and conventional one-tube nested PCR for specific detection of CSFV were compared. Additionally, two methods designed for detection of all pestivirus species real-time SYBR Green I and one-tube nested PCR were included into the study. Analyzed methods varied considerably regarding their sensitivity and specificity, what suggests that careful selection of diagnostic methods and their evaluation on a regular basis is necessary.
Becher P, Avalos Ramirez R, Orlich M, Cedillo Rosales S, Konig M, Schweizer M, Stalder H, Schirrmeier H, Thiel HJ (2003) Genetic and antigenic characterization of novel pestivirus genotypes: implications for classification. Virology 311: 96-104.
Bingham PC, McFadden AM, Wang J, Kittelberger R, Clough RR, Tham KM (2010) Investigation of a pig herd with animals seropositive for classical swine fever and where porcine circovirus-associated disease had been diagnosed. N Z Vet J 58: 253-259.
de Smit AJ, Eble PL, de Kluijver EP, Bloemraad M, Bouma A (1999) Laboratory decision-making during the classical swine fever epidemic of 1997-1998 in The Netherlands. Prev Vet Med 42: 185-199.
Hoffmann B, Beer M, Schelp C, Schirrmeier H, Depner K (2005) Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever. J Virol Methods 130: 36-44.
Katz JB, Ridpath JF, Bolin SR (1993) Presumptive diagnostic differentiation of hog cholera virus from bovine viral diarrhea and border disease viruses by using a cDNA nested-amplification approach. J Clin Microbiol 31: 565-568.
Loeffen WL, van Beuningen A, Quak S, Elbers AR (2009) Seroprevalence and risk factors for the presence of ruminant pestiviruses in the Dutch swine population. Vet Microbiol 136: 240-245.
Oguzoglu TC, Floegel-Niesmann G, Frey HR, Moennig V (2001) Differential diagnosis of classical swine fever and border disease: seroepidemiological investigation of a pestivirus infection on a mixed sheep and swine farm. Dtsch Tierarztl Wochenschr 108: 210-213.
Passler T, Walz PH (2010) Bovine viral diarrhea virus infections in heterologous species. Anim Health Res Rev 11: 191-205.
Paton DJ, Done SH (1994) Congenital infection of pigs with ruminant-type pestiviruses. J Comp Pathol 111: 151-163.
Paton DJ, McGoldrick A, Greiser-Wilke I, Parchariyanon S, Song JY, Liou PP, Stadejek T, Lowings JP, Bjorklund H, Belak S (2000) Genetic typing of classical swine fever virus. Vet Microbiol 73: 137-157.
Podgorska K, Stadejek T (2010) Optimisation of reverse transcription can improve the sensitivity of RT-PCR for detection of classical swine fever virus. Acta Vet Hung 58: 257-264.
Polak MP, Zmudzinski JF (1999) Prevalence of bovine viral diarrhea virus infection in bulls in artificial insemination centers in Poland. Vet Microbiol 64: 253-257.
Ribbens S, Goris N, Neyts J, Dewulf J (2012) Classical swine fever outbreak containment using antiviral supplementation: a potential alternative to emergency vaccination and stamping-out. Prev Vet Med 106: 34-41.
Stadejek T, Pejsak Z (2000) Diagnosis of pestivirus infections of pigs by simplified RT-nested PCR. Med Weter 56: 121-124.
Stadejek T, Podgórska K, Pejsak Z (2006) Applying Real Time PCR for detecting pestiviruses. Med Weter 62: 165-169.
Stegeman A, Elbers A, de Smit H, Moser H, Smak J, Pluimers F (2000) The 1997-1998 epidemic of classical swine fever in the Netherlands. Vet Microbiol 73: 183- -196.
Uttenthal A, Storgaard T, Oleksiewicz MB, de Stricker K (2003) Experimental infection with the Paderborn isolate of classical swine fever virus in 10-week-old pigs: determination of viral replication kinetics by quantitative RT-PCR, virus isolation and antigen ELISA. Vet Microbiol 92: 197-212.
Vilcek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ (1994) Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol 136: 309-323.
Wieringa-Jelsma T, Quak S, Loeffen WL (2006) Limited BVDV transmission and full protection against CSFV transmission in pigs experimentally infected with BVDV type 1b. Vet Microbiol 118: 26-36.