Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins

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Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.

1. Acton J.C., Breyer L., Satterlee L.D., Effect of dietary fiber constituents on the in vitro digestibility of casein. J. Food Sci., 1982, 47, 556–560.

2. Andersen K.E., Bagger C., Sørensen H., Sørensen J.C., A process for the manufacture of products from cruciferous crops. Denmark, Patent no. WO/2012/149941, 2012, Available from: [https://patents.google.com/patent/WO2012149941].

3. AOAC International, Official Method 985.29. Total dietary fiber in foods. 1985, in: Official Methods of Analysis of AOAC International, Gaithersburg, MD, USA.

4. AOAC International, Official Method 950.46 (Modified). Moisture in meat. 2000, in: Official Methods of Analysis of AOAC International, Gaithersburg, MD, USA.

5. Asp N.G., Johansson C.G., Hallmer H., Siljeström M., Rapid enzymatic assay of insoluble and soluble dietary fiber. J. Agric. Food Chem., 1983, 31, 476–482.

6. Bjergegaard C., Eggum B.O., Jensen S.K., Sørensen H., Dietary fibres in oilseed rape: Physiological and antinutritional effects in rats of isolated IDF and SDF added to a standard diet. J. Anim. Physiol. Anim. Nutr., 1991a, 66, 69–79.

7. Bjergegaard C., Jensen S.K., Sørensen H., Dietary fibres in oilseed rape: Properties and effects on the digestibility of rapeseed meal. GCIRC Congr. Saskat. Can., 1991b, 2, 448–453.

8. Bjergegaard C., Sørensen H., Sørensen S., Dietary fibres and associated compounds in rape seed and biorefined rape seed products compared to DF in pea. J. Anim. Feed Sci., 1997, 6, 163–184.

9. Bos C., Airinei G., Mariotti F., Benamouzig R., Bérot S., Evrard J., Fénart E., Tomé D., Gaudichon C., The poor digestibility of rapeseed protein is balanced by its very high metabolic utilization in humans. J. Nutr., 2007, 137, 594–600.

10. Brulé D., Savoie L., In vitro digestibility of protein and amino acids in protein mixtures. J. Sci. Food Agric., 1988, 43, 361–372.

11. Campbell L., Rempel C.B., Wanasundara J.P.D., Canola/rapeseed protein: Future opportunities and directions — Workshop proceedings of IRC 2015. Plants, 2016, 5, art. no. 17.

12. Danielsen V., Eggum B.O., Jensen S.K., Sørensen H., Dehulled protein-rich rapeseed meal as a protein source for early weaned piglets. Anim. Feed Sci. Technol., 1994, 46, 239–250.

13. Dutta S.K., Hlasko J., Dietary fiber in pancreatic disease: effect of high-fiber diet on fat malabsorption in pancreatic insufficiency and in vitro study of the interaction of dietary fiber with pancreatic enzymes. Am. J. Clin. Nutr., 1985, 41, 517–525.

14. Fleddermann M., Fechner A., Rößler A., Bähr M., Pastor A., Liebert F., Jahreis G., Nutritional evaluation of rapeseed protein compared to soy protein for quality, plasma amino acids, and nitrogen balance – A randomized cross-over intervention study in humans. Clin. Nutr., 2013, 32, 519–526.

15. Gagne C.M., Acton J.C., Fiber constituents and fibrous food residue effects on the in vitro enzymatic digestion of protein. J. Food Sci., 1983, 48, 734–738.

16. Goodman B.E., Insights into digestion and absorption of major nutrients in humans. Adv. Physiol. Educ., 2010, 34, 44–53.

17. Grala W., Verstegen M.W., Jansman A.J., Huisman J., van Leeusen P., Ileal apparent protein and amino acid digestibilities and endogenous nitrogen losses in pigs fed soybean and rapeseed products. J. Anim. Sci., 1998, 76, 557–568.

18. Grundy M.M.-L., Edwards C.H., Mackie A.R., Gidley M.J., Butterworth P.J., Ellis P.R., Re-evaluation of the mechanisms of dietary fibre and implications for macronutrient bioaccessibility, digestion and postprandial metabolism. Br. J. Nutr., 2016, 116, 816–833.

19. Hansen W.E., Effect of dietary fiber on proteolytic pancreatic enzymes in vitro. Int. J. Pancreatol., 1986, 1, 341–351.

20. Ivanova P., Chalova V., Uzunova G., Koleva L., Manolov I., Biochemical characterization of industrially produced rapeseed meal as a protein source in food industry. Agric. Agric. Sci. Procedia, 2016, 10, 55–62.

21. Joehnke M.S., Rehder A., Sørensen S., Bjergegaard C., Sørensen J.C., Markedal K.E., In vitro digestibility of rapeseed and bovine whey protein mixtures. J. Agric. Food Chem., 2018, 66, 711–719.

22. Khajali F., Slominski B.A., Factors that affect the nutritive value of canola meal for poultry. Poult. Sci., 2012, 91, 2564–2575.

23. Kracht W., Dänicke S., Kluge H., Keller K., Matzke W., Hennig U., Schumann W., Effect of dehulling of rapeseed on feed value and nutrient digestibility of rape products in pigs. Arch. Anim. Nutr., 2004, 58, 389–404.

24. Mansour E.H., Dworschák E., Lugasi A., Gaál Ö., Barna É., Gergely A., Effect of processing on the antinutritive factors and nutritive value of rapeseed products. Food Chem., 1993, 47, 247–252.

25. Mosenthin R., Sauer W.C., Ahrens F., Dietary pectin’s effect on ileal and fecal amino acid digestibility and exocrine pancreatic secretions in growing pigs. J. Nutr., 1994, 124, 1222–1229.

26. Ochodzki P., Rakowska M., Rek-Cieply B., Bjergegaard C., Sørensen H., Studies on enzymatic fractionation, chemical composition and biological effects of dietary fibre in rape seed (Brassica napus L.). 2. Influence of rape seed dietary fibre on digestibility of protein and organic matter using unprocessed and heated full fat rape seed and isolated dietary fibre fractions added to rat diets. J. Anim. Feed Sci., 1995, 4, 139–151.

27. Pasha I., Saeed F., Sultan M.T., Khan M.R., Rohi M., Recent developments in minimal processing: A tool to retain nutritional quality of food. Crit. Rev. Food Sci. Nutr., 2014, 54, 340–351.

28. Ren L.Q., Zhao F., Tan H.Z., Zhao J.T., Zhang J.Z., Zhang H.F., Effects of dietary protein source on the digestive enzyme activities and electrolyte composition in the small intestinal fluid of chickens. Poult. Sci., 2012, 91, 1641–1646.

29. Richter C.K., Skulas-Ray A.C., Champagne C.M., Kris-Etherton P.M., Plant protein and animal proteins: Do they differentially affect cardiovascular disease risk?. Adv. Nutr., 2015, 6, 712–728.

30. Saunders R.M., Betschart A.A., The significance of protein as a component of dietary fiber. Am. J. Clin. Nutr., 1980, 33, 960–961.

31. Savoie L., Galibois I., Parent G., Charbonneau R., Sequential release of amino acids and peptides during in vitro digestion of casein and rapeseed proteins. Nutr. Res., 1988, 8, 1319–1326.

32. Schulze H., van Leeuwen P., Verstegen M.W., Huisman J., Souffrant W.B., Ahrens F., Effect of level of dietary neutral detergent fiber on ileal apparent digestibility and ileal nitrogen losses in pigs. J. Anim. Sci., 1994, 72, 2362–2368.

33. Shah N., Atallah M.T., Mahoney R.R., Pellett P.L., Effect of dietary fiber components on fecal nitrogen excretion and protein utilization in growing rats. J. Nutr., 1982, 112, 658–666.

34. Slominski B.A., Jia W., Rogiewicz A., Nyachoti C.M., Hickling D., Low-fiber canola. Part 1. Chemical and nutritive composition of the meal. J. Agric. Food Chem., 2012, 60, 12225–12230.

35. Stone A.K., Teymurova A., Dang Q., Abeysekara S., Karalash A., Nickerson M.T., Formation and functional attributes of electrostatic complexes involving napin protein isolate and anionic polysaccharides. Eur. Food Res. Technol., 2014, 238, 773–780.

36. Tan S.H., Mailer R.J., Blanchard C.L., Agboola S.O., Canola proteins for human consumption: Extraction, profile, and functional properties. J. Food Sci., 2011, 76, R16–R28.

37. USDA FAS, Oilseeds: World Markets and Trade, 2018, Available from: [https://apps.fas.usda.gov/psdonline/circulars/oilseeds.pdf. Last accessed 04/06/2018].

38. Wanasundara J.P.D., Proteins of Brassicaceae oilseeds and their potential as a plant protein source. Crit. Rev. Food Sci. Nutr., 2011, 51, 635–677.

39. Zhang B., Liu G., Ying D., Sanguansri L., Augustin M.A., Effect of extrusion conditions on the physico-chemical properties and in vitro protein digestibility of canola meal. Food Res. Int., 2017, 100, 658–664.

Polish Journal of Food and Nutrition Sciences

The Journal of Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn

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