Distribution of (1,3)(1,4)-β-D-Glucans in Grains of Polish Oat Cultivars and Lines (Avena sativa L.) – Short Report

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The distribution of a fraction of soluble dietary fibre (1,3)(1,4)-β-D-glucans was depicted in selected Polish oat grains (Avena sativa L.). Localisation of β-glucans within the grains was visualised by the light microscopy with Calcofluor white as a fluorescence agent. The content of β-glucans varied in samples from 3.08% d.m. to 5.04% d.m. Analysis of distribution of (1,3)(1,4)-β-D-glucans showed that the localization of β-glucans varied between various cultivars and lines. It was demonstrated that the total content of (1,3)(1,4)-β-D-glucans in oat kernels had an effect on their distribution. All the lines and cultivars tested displayed the greatest accumulation of (1,3)(1,4)-β-D-glucans in the cells of the subaleurone layer. With increase in the levels of β-glucans in high-glucan oat cultivars and lines, a tendency was observed towards their greater accumulation in the central parts of the kernel. It makes oat grain particularly suitable for the production of wholemeal foods. It is important not only to focus on increasing the content of β-glucans, but also to investigate molecules distribution in the seed. It was also demonstrated that Dukat cultivar was characterised by an especially valuable triple aleurone layer, which makes this cultivar predestined for further breeding studies as an extremely valuable carrier of genetic information.

1. AACC Approved Methods, 10th edition, 2000.

2. Åman P., Rimsten L., Andersson R., Molecular weight distribution of β-glucan in oat-based foods. Cereal Chem., 2004, 81, 356–360.

3. Andersson A.A.M., Börjesdotter D., Effects of environment and variety on content and molecular weight of β-glucan in oats. J. Cereal Sci., 2011, 54, 122–128.

4. Andersson K.E., Svedberg K.A., Lindholm M.W., Öste R., Hellstrand P., Oats (Avena sativa) reduce atherogenesis in LDL-receptor-deficient mice. Atherosclerosis, 2010, 212, 93–99.

5. Bechtel D.B., Pomeranz Y., Ultrastructure and cytochemistry of mature oat (Avena sativa L.) endosperm. The aleurone layer and starchy endosperm. Cereal Chem., 1981, 58, 61–69.

6. Chang H.C., Huang C.N., Yeh D.M., Wang S.J., Peng C.H., Wang C.J., Oat prevents obesity and abdominal fat distribution, and improves liver function in humans. Plant Foods Hum. Nutr., 2013, 68, 18–23.

7. Colleoni-Sirghie M., Jannink J.-L., White P.J., Pasting and thermal properties of flours from oat lines with high and typical amounts of β-glucan. Cereal Chem., 2004, 81, 686–692.

8. Czubaszek A., Karolini-Skaradzińska Z., Effects of wheat flour supplementation with oat products on dough and bread quality. Pol. J. Food Nutr. Sci., 2005, 14/55, 3, 281–286.

9. Delaney B., Carlson T., Frazer S., Zheng T., Hess R., Ostergren K., Kierzek K., Haworth J., Knutson N., Junker K., Jonker D., Evaluation of the toxicity of concentrated barley β-glucan in a 28-day feeding study in Wistar rats. Food Chem. Toxicol., 2003, 41, 477–487.

10. Frank J., Sundberg B., Kamal-Eldin A., Vessby B., Åman P., Yeast-leavened oat breads with high or low molecular weight β-glucan do not differ in their effects on blood concentrations of lipids, insulin, or glucose in humans. J. Nutr., 2004, 134, 1384–1388.

11. Fulcher R.G., Miller S.S., Structure of oat bran and distribution of dietary fiber components. 1993, In: Oat Bran (ed. P.J. Wood.). AACC Inc., St. Paul, Minnesota, USA, pp. 1–24.

12. Gajdošová A., Petruláková Z., Havrlentová M., Červená V., Hozová B., Šturdík E., Kogan G., The content of water-soluble and water-insoluble β-D-glucans in selected oats and barley varieties. Carboh. Polym., 2007, 70, 46–52.

13. Gąsiorowski H., Cierniewska A., Morfologia i anatomia. 1995, In: Oat. Chemistry and Technology (ed. H. Gąsiorowski). PWRL, Poznań, pp. 36–46.

14. Kerckhoffs D., Hornstra G., Mensink R.P., Cholesterol-lowering effect of β-glucan from oat bran in mildly hypercholesterolemic subjects may decrease when β-glucan is incorporated into bread and cookies. Am. J. Clin. Nutr., 2003, 78, 221–227.

15. Lazaridou A., Papoutsi Z., Biliaderis C.G., Moutsatsou P., Effect of oat and barley β-glucans on inhibition of cytokine-induced adhesion molecule expression in human aortic endothelial cells: Molecular structure-function relations. Carboh. Polym., 2011, 84, 153–161.

16. Liu L., Zubik L., Collins F.W., Marko M., Meydani M., The antiatherogenic potential of oat phenolic compounds. Atherosclerosis, 2004, 175, 39–49.

17. Miller S.S., Fulcher R.G., Distribution of (1,3)(1,4)-β-D-glucan in kernels of oats and barley using microspectrofluorometry. Cereal Chem., 1994, 71, 64–68.

18. Miller S.S., Fulcher R.G., Sen A., Arnason J.T., Oat endosperm cell walls: I. Isolation, composition, and comparison with other tissues. Cereal Chem., 1995, 72, 421–427.

19. Redaelli R., Del Frate V., Bellato S., Terracciano G., Ciccoritti R., Germeier Ch.U., De Stefanis E., Sgrulleta D., Genetic and environmental variability in total and soluble β-glucan in European oat genotypes. J. Cereal Sci., 2013, 57, 193–199.

20. Queenan K.M., Stewart M.L., Smith K.N., Thomas W., Fulcher G., Slavin J.L., Concentrated oat β-glucan, a fermentable fiber, lowers serum cholesterol in hypercholesterolemic adults in a randomized controlled trial. Nutr. J., 2007, 6, 6.

21. Sayar S., Jannink J.-L., White P.J., Digestion residues of typical and high-β-glucan oat flours provide substrates for in vitro fermentation. J. Agric. Food Chem., 2007, 55, 5306–5311.

22. Sikora P., Tosh S.M., Brummer Y., Olsson O., Identification of high β-glucan oat lines and localization and characterization of their seed kernel β-glucans. Food Chem., 2013, 137, 83–91.

23. Skendi A., Biliaderis C.G., Lazaridou A., Izydorczyk M.S., Structure and rheological properties of water soluble β-glucans from oat cultivars of Avena sativa and Avena bysantina. J. Cereal Sci., 2003, 38, 15–31.

24. Sobczyk M., Effect of various oat forms on the quality of confectionery. Pol. J. Food Nutr. Sci., 2008, 58, 3, 301–305.

25. Sykut-Domańska E., Rzedzicki Z., Nita Z., Chemical composition variability of naked and husked oat grain (Avena sativa L.). Cereal Res. Commun., 2013, 41, 327–337.

26. Weickert M.O., Mohlig M., Schofl Ch., Arafat A., Otto B., Viehoff H., Koebnick C., Kohl A., Spranger J., Pfeiffer A., Cereal fiber improves whole-body insulin sensitivity in overweight and obese women. Diab. Care, 2006, 29, 775–780.

27. Wood P.J., Physicochemical characteristics and physiological properties of oat (1,3)(1,4)-β-D-glucan. 1993, In: Oat Bran (ed. P.J. Wood), AACC, St. Paul, Minnesota, USA, pp. 83–112.

28. Volman J.J., Mensink R.P., Ramakers J.D., de Winther M.P., Carlsen H., Blomhoff R., Buurman W.A., Plat J., Dietary (1–3) (1–4)-β-D-glucans from oat activate nuclear factor-κB in intestinal leukocytes and enterocytes from mice. Nutr. Res., 2010, 30, 40–48.

29. Volman J.J., Mensink R.P., Buurman W.A., Plat J., In vivo effects of dietary (1–3)(1–4)-β-D-glucans from oat on mucosal immune responses in man and mice. Scand. J. Gastroent., 2011, 46, 603–610.

30. Zhao Q., Hu X., Guo Q., Cui S.W., Xian Y., You S., Chen X., Xu Ch., Gao X., Physicochemical properties and regulatory effects on db/db diabetic mice of β-glucans extracted from oat, wheat and barley. Food Hydrocoll, 2014, 37, 60–68.

Polish Journal of Food and Nutrition Sciences

The Journal of Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn

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